Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Methods ; 125: 16-24, 2017 08 01.
Article in English | MEDLINE | ID: mdl-28669867

ABSTRACT

Here we describe the synthesis and use of a directed hydroxyl radical probe, tethered to a pre-mRNA substrate, to map the structure of this substrate during the spliceosome assembly process. These studies indicate an early organization and proximation of conserved pre-mRNA sequences during spliceosome assembly. This methodology may be adapted to the synthesis of a wide variety of modified RNAs for use as probes of RNA structure and RNA-protein interaction.


Subject(s)
Hydroxyl Radical/chemistry , Molecular Probe Techniques , Molecular Probes/chemical synthesis , RNA Precursors/chemistry , Spliceosomes/metabolism , Crystallography, X-Ray/methods , DNA-Directed RNA Polymerases/metabolism , Hydroxyl Radical/metabolism , Oligonucleotides/metabolism , RNA Precursors/metabolism , RNA Splicing , Spliceosomes/chemistry , Viral Proteins/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...