ABSTRACT
The HIV-1 genetic variants circulated in the Asian part of the Russian Federation in 2005-2010 were studied. The samples of HIV-1 (427 in total) were collected in Khabarovsk, Magadan, Kurgan, Krasnoyarsk, Noyabr'sk, Yakutsk, Altay, and Tyva. Sequencing of some genome regions followed by the phylogenetic analysis or specific Internet resource sampling were used as the main methods of the HIV subtyping. The domination of the IDU-A HIV-1 genetic variant typical of HIV-infection epidemic in Russia was shown in all regions tested in 2005-2010. This variant prevailed both in IDUs and heterosexuals. In addition to IDU-A, some other HIV-1 genetic variants were found among them: subtype B and recombinant CRF03_AB. The HIV-1 genetic polymorphism in Russia was found to be low. An increase in the genetic distance among studied de novo samples was noted in the Asian part of Russia in 2005-2010 (26-68%) as compared to the European variants in 1996-1999 (10%).
Subject(s)
HIV Infections/epidemiology , HIV Infections/genetics , HIV-1/genetics , Phylogeny , Polymorphism, Genetic , Female , Humans , Male , Retrospective Studies , Siberia/epidemiologyABSTRACT
The amino acid sequence of the known strain HXB-2 belonging to subtype B is used as a reference strain matrix to determine HIV genotypic resistance by the sequencing technique. However, numerous studies have ascertained that HIV non-B subtypes have been widely accepted in Russia. The authors' data show that the significance of low score mutations in the Russian populations of HIV-1 is yet to be explained, unquestionably, arouses great scientific interest, and calls for further study.
Subject(s)
Anti-HIV Agents/pharmacology , HIV-1/drug effects , Adolescent , Adult , Child , Child, Preschool , Drug Resistance, Viral , Female , HIV-1/genetics , Humans , Infant , Male , Middle Aged , Mutation , Siberia , Young AdultABSTRACT
In order to detect mutations in protease and reverse transcriptase regions determining the resistance of human immunodeficiency virus (HIV) to antiretroviral drugs, sequencing of pol gene was used. Itwas concluded that HIV epidemic in Yamal region is characterized by heterogeneity of HIV-1 subtype population with trend to domination of recombinant and ground variants and spreading of mutant Mut-77 strain. Obtained results demonstrate the possibility of identification of mutations as epidemiological markers which permits their use for epidemiologic investigations.
