ABSTRACT
Karyotypes of the hybrid cell lines NS-RL-3 (TK- -sheep kidney cells and rabbit lymphocytes) and betaCR-NS (TK- -rabbit beta-cells and TK- -sheep kidney cells) were investigated. It was shown that both hybrid cell lines were characterized by presence of both sheep and rabbit chromosomes, which number and structure varies depending on the cell type and the number of passages. In some cases the aberrant chromosomes were identified. It was observed, that 40-50% of the NS-RL-3 cells survived in culture in the presence of the human blood serum, and also were identified during 7-28 days after their introduction into the organism of the animal. Thus, the partial immunological tolerance of the hybrid cell lines has been suggested.
Subject(s)
Hybrid Cells/cytology , Hybrid Cells/immunology , Rabbits , Sheep , Animals , Cell Line , Chromosome Aberrations , Chromosomes, Mammalian/genetics , Humans , Immune Tolerance , Karyotyping , Kidney , Lymphocytes , Pancreas , Species SpecificityABSTRACT
Mammogenesis of rabbits was studied in dynamics, including periods of sexual maturity (3-6 months old), pregnancy, lactation and involution. A characteristic of histological structure of mammary gland of rabbits in different physiological periods was done. Variation in maintenance of glandular tissue of mammary gland during mammogenesis was analyzed. It has been shown that the share of parenchyma tissue is increased with the pregnancy onset and achieves its maximum value by the 25-30 th days of pregnancy. The increase in densities of mammocyte nucleus in this period and also in the period preceding the sexual maturity was observed that is associated with the active proliferation of cells. It is shown that an in vitro cell culture, derived during the period preceding the sexual maturity, has a great grown potential, whereas a culture devired in the course of lactogenesis is characterized by the presence of highly specific lactocytes and has a low proliferative activity.
