ABSTRACT
AIM: Autofluorescence of experimental tumor (hepatoma A22 (MH-A22)) was employed to discriminate the optical differences between necrotic and non-necrotic tumor, hemorrhagic tumor and healthy tissue. METHODS: The experiment was performed ex vivo using the transplantable tumor from the right haunch of hybrid mice (C57Bl/CBA). Blue LED light (lambda em=405 nm) was applied for autofluorescence excitation and fibre optics based spectrofluorimeter was used for spectra detection. RESULTS: We observed that necrotic tumor tissue is characterized by the absence of endogenous porphyrins fluorescence, and registered spectra do not possess differences in the red spectral region (600-710 nm) in comparison with normalized autofluorescence spectra of muscle. Moreover, only certain segments of non-necrotic tumor bear the fluorescence of endogenous porphyrins. CONCLUSIONS: Based on the experimental results, we suggest that the absence of long-waved fluorescence differences between necrotic tumor tissue and healthy tissue, e.g. muscle can impede the demarcation between healthy and tumor tissue. The uneven distribution of endogenous porphyrins in non-necrotic tumor tissue as well as the absence of endogenous porphyrins fluorescence in the small experimental tumors complicates the localization of cancerous tissue based on the autofluorescence registration.
Subject(s)
Fluorescence , Liver Neoplasms, Experimental/diagnosis , Animals , Biopsy , Chimera , Female , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Necrosis , Spectrometry, Fluorescence , Tumor Cells, Cultured/transplantationABSTRACT
Villous adenoma comprises 5.6% of colon tumors. Adenomas of the colon could be divided into tubular, villous, and tubulovillous adenomas. Villous adenomas are not as common as tubular neoplasms; however, usually they are larger and could cause a depleting syndrome characterized by dehydration, hyponatremia, hypokalemia, hypochloremia, and metabolic acidosis. In severe cases, shock and renal failure in older patients could cause death. Case of villous adenoma is presented in this article. Seventy-eight-year-old male who suffered permanent diarrhea for few years followed with acute renal failure. Villous adenoma was diagnosed. The conservative treatment had short renal function improvement. Hemodialysis had to be performed. Renal function was completely recovered after radical tumor surgery.
Subject(s)
Acute Kidney Injury/etiology , Adenoma, Villous/complications , Colonic Neoplasms/complications , Acute Kidney Injury/diagnosis , Acute Kidney Injury/therapy , Adenoma, Villous/diagnosis , Adenoma, Villous/surgery , Aged , Colectomy , Colonic Neoplasms/diagnosis , Colonic Neoplasms/surgery , Diarrhea/etiology , Humans , Kidney Function Tests , Male , Renal Dialysis , Time FactorsABSTRACT
An International Agency for Research on Cancer (IARC) committee recognized aerosol of sulphuric acid as a human carcinogen on the basis of epidemiological studies. No experimental studies on the carcinogenicity, either of sulfuric acid aerosol or of sulfuric acid itself was available. Our aim was to determine whether sulfuric acid is a causal or modifying factor in carcinogenesis, especially in the respiratory tract. We used two species of laboratory animals (both sexes) 315 Wistar rats and 219 CBAxC57Bl mice in a long term experimental study. The rats were treated with sulfuric acid (maximal tolerated doses, by chronic intratracheal instillations or by gastric intubations) and/or benzo(a)pyrene (by intratracheal instillations). The mice were treated with sulfuric acid (by chronic gastric intubations) and/or urethane (by intraperitoneal injections). We observed the animals throughout their lives and performed gross and microscopic examination of all organs. The results of the first year of study did not provide clear evidence either for sulfuric acid carcinogenicity or for co-carcinogenicity. However, in the second year tumors appeared in those organs where sulfuric acid acted directly. A modifying (stimulating) effect of sulfuric acid on carcinogenesis induced with benzo(a)pyren was observed in rats. Sulfuric acid did not influence lung carcinogenesis induced with urethane in mice.
ABSTRACT
Long-term carcinogenicity of Ftorafur (Tegafur) was studied in rodents. Rats and mice were treated for one year per os with 40 (mice) and 60 (rat) mg/kg Ftorafur twice a week and were followed for their entire life. Analysis of the data provide no evidence for the carcinogenicity of Ftorafur in rodents. These findings are similar to other antimetabolite studies and contrasts with the carcinogenic alkylating agents.
