ABSTRACT
BACKGROUND: Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera is more productive for antigen isolation, SEREX-analysis was applied to four cases of stages II-IV primary colon tumor and 22 new antigens were isolated. OBJECTIVE: To characterize 22 primary colon cancer antigens isolated by SEREX-technique. MATERIALS AND METHODS: Allogenic screening, real-time PCR analysis. RESULTS: After allogeneic immunoscreening, for 5 of 22 (22%) isolated antigens were confirmed colon cancer restricted serological profile solely positive for 14% of tested colon cancer sera. Through these five antigens, KY-CC-17/ß-actin has cytoskeleton function; KY-CC-14/ACTR1A and KY-CC-19/TSGA2 participate in chromosome segregation; KY-CC-12/FKBP4 regulates steroid receptor function and KY-CC-15/PLRG1 is a component of spliceosome complex. For the last four antigens tested were found aberrant mRNA expression in some cases of colon tumor. CONCLUSION: The exploration of identified antigens may define suitable targets for immunotherapy or diagnostic of colon cancer.
Subject(s)
Antigens, Neoplasm/immunology , Colonic Neoplasms/immunology , Adult , Antigens, Neoplasm/blood , Antigens, Neoplasm/genetics , Biomarkers, Tumor , Colonic Neoplasms/blood , Colonic Neoplasms/genetics , Female , Gene Expression , Gene Library , Humans , Immunoassay/methods , Male , Middle AgedABSTRACT
The data on surface enhanced IR absorption (SEIRA) of nucleic acids deposited on a metal substrate were obtained using FTIR in reflectance mode. A 200-400 A thick gold film on a glass plate was the metal substrate. The approximate enhancement factors of the SEIRA for different vibrations of nucleic acids in our experimental conditions were 3-5. The roughness of the Au surface was about 50 A. Application of this method to nucleic acids isolated from tumor cells revealed some possible peculiarities of their structural organization, namely, the appearance of unusual sugar and base conformations, modification of the phosphate backbone, redistribution of the H-bond net, and so forth. This method enhanced a set of the bands, which is impossible to observe in conventional IR geometry. The SEIRA spectra of the RNA from tumor cells showed more sensitivity to the grade of tumor malignancy than the spectra of the DNA. After application of the anticancer drug doxorubicin to sensitive and resistant strains, the DNA isolated from these strains had different spectral features, especially in the region of the phosphate I and II bands. As induced by anticancer drugs, the conformational changes in the DNA from resistant and sensitive cancer strains could be characterized with different levels of structure disordering.
Subject(s)
DNA, Neoplasm/chemistry , RNA, Neoplasm/chemistry , Spectroscopy, Fourier Transform Infrared/methods , Animals , Breast Neoplasms/chemistry , Female , Humans , Nucleic Acid Conformation , Rats , Tumor Cells, CulturedABSTRACT
Fourier transform infrared (FTIR) spectra of RNA isolated from tumour brain (glioma) and DNA isolated from low-dose gamma-irradiated epididymis cells of rats from the Chernobyl accident zone have been investigated in the 700-4000 cm(-1) region. The total absorbed radiation doses were equal to or less than 57 cGy. Observed changes in the FTIR spectra represent the damage in the primary, secondary and tertiary structure of nucleic acid, which seem to be connected with modification of bases and sugars, and redistribution of the H-bond network. FTIR data for tumour and gamma-irradiated nucleic acid show some similar features. A great amount of statistical data and good mathematical approaches are needed for the use of these data as diagnostic criteria.
