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Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells.
Birkbeck, T Harry; Griffen, Angela A; Reid, Helen I; Laidler, L Anthony; Wadsworth, Simon.
Infect Immun ; 72(6): 3693-4, 2004 Jun.
Article in English | MEDLINE | ID: mdl-15155687

ABSTRACT

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.


Subject(s)
Piscirickettsiaceae/growth & development , Salmo salar/microbiology , Spodoptera/microbiology , Animals , Bacteriological Techniques , Cells, Cultured , Fish Diseases/microbiology , Fish Diseases/mortality , Piscirickettsiaceae/pathogenicity , Piscirickettsiaceae Infections/microbiology , Piscirickettsiaceae Infections/veterinary , Virulence
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