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Methods Mol Biol
; 1266: 1-6, 2015.
Article
in English
| MEDLINE
| ID: mdl-25560064
ABSTRACT
The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.