ABSTRACT
Mesenchymal stromal cells (MSC) are considered to be promising tool of regenerative medicine. Migration of MSC toward damaged inflammatory site is essential for physiological tissue reparation. Therefore we studied modifications of migratory features of adipose tissue derived MSC (AT-MSC) after co-cultivation with activated monocytes derived from THP-1 cell line. As a result, we have observed an increased migration rate of AT-MSC in vitro in the absence of chemoattractant gradient as well as toward the gradient of PDGF BB (platelet-derived growth factor BB), which is well known chemoattractant for the cells of mesenchymal origin. Furthermore, the rate of directional AT-MSC migration through fibronectin was also increased. We have established that signaling from PDGFRß which is activated through binding of integrin receptors with extracellular matrix may be possible way to stimulate cellular migration under simulated inflammatory conditions.
Subject(s)
Chemotaxis/drug effects , Extracellular Matrix/genetics , Mesenchymal Stem Cells/drug effects , Monocytes/drug effects , Proto-Oncogene Proteins c-sis/pharmacology , Receptor, Platelet-Derived Growth Factor beta/genetics , Adipose Tissue/cytology , Adipose Tissue/metabolism , Adult , Becaplermin , Cell Differentiation , Cell Line , Cells, Cultured , Coculture Techniques , Extracellular Matrix/metabolism , Gene Expression , Humans , Integrin alpha Chains/genetics , Integrin alpha Chains/metabolism , Integrin beta Chains/genetics , Integrin beta Chains/metabolism , Mesenchymal Stem Cells/cytology , Mesenchymal Stem Cells/metabolism , Middle Aged , Monocytes/cytology , Monocytes/metabolism , Protein Binding , Receptor, Platelet-Derived Growth Factor beta/agonists , Receptor, Platelet-Derived Growth Factor beta/metabolism , Signal Transduction , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
Retrospective comparative analysis of therapy of the rheumatoid arthritis was undertaken. The following variants of antiphlogistic prescription: inactive tactics (long-lasting administration of one medicine), tactics of protracted treatment (separate courses of disease-modifying antirheumatic drugs with long breaks between them), tactics of first aid (at the beginning "passive treatments", after the appearance of complications begun active therapy with cytostatics); tactics of pyramid (the order of the prescription of medicines corresponds to classical strategy of baseline therapy with short breaks during the treatment), tactics of active therapy (the early beginning of baseline therapy with consistent change of medicine); effectiveness and safety of long-lasting administration of antiphlogistic in complex therapy of the rheumatoid arthritis.
Subject(s)
Antirheumatic Agents/administration & dosage , Arthritis, Rheumatoid/drug therapy , Cytostatic Agents/administration & dosage , Adult , Arthritis, Rheumatoid/diagnosis , Female , Humans , Male , Middle AgedABSTRACT
By the example of rheumatoid arthritis was considered the methods of economic assessment of advisability of modern biological medication usage in military medicine. The usage of these medications leads to quick clinical response, decrease of the additional medication intake, decrease of the average duration of treatment, "move aside" time of permanent disablement. Modern active therapy can increase the effectiveness of treatment; improve the quality of life, decrease the average duration of hospital stay, following treatment expense and welfare payment for this category of ill.
