ABSTRACT
AIM: To evaluate microviscosity and sorption capacity of erythrocyte membranes (SCEM) from patients with laryngeal cancer (LC). MATERIALS AND METHODS: Samples from 35 patients with LC of stages II and III and 20 healthy volunteers were investigated by electron paramagnetic resonance with Bis(1-oxyl-2,2,6,6-tetramethylpiperidinyl-4)-ester of 5,7-dimethyladamantane-1,3-dicarbonic acid (AdTEMPO) probe. SCEM was evaluated by amount of unabsorbed methylene blue. RESULTS: Microviscosity of erythrocyte membranes was determined by the effective rotational diffusion correlation times (τeff) and a decrease in radical spectrum signal intensity per hour. The most apparent decrease in mobility of the AdTEMPO in erythrocytes was observed prior to washing of erythrocytes with 0.9% NaCl for 5 min after probe insertion. The deceleration after 60 min was observed only in stage II LC. τeff was at control values after washing of erythrocytes of stage II LC 5 min after probe insertion and was significantly reduced in stage III LC in comparison to control. Radical spectrum signal intensity per hour in samples of stage II and III patients prior to and after washing of erythrocytes was on average 1.5-fold higher than that of control. SCEM in samples of stage II and III LC was found in 40 and 33% cases, respectively and was on average significantly reduced in comparison to control. CONCLUSIONS: The initial interaction of AdTEMPO with erythrocyte membranes of stage II and III LC patients is accompanied by an increase in τeff, indicating deceleration of probe rotation. τeff of the probe in membranes remains unchanged in 60 min, indicating changes in the structural organization of lipid bilayer and its associated proteins in particular. The similarity of SCEM for both studied groups reflects the pathological changes in function of erythrocyte membranes.
Subject(s)
Carcinoma, Squamous Cell/metabolism , Electron Spin Resonance Spectroscopy/methods , Erythrocyte Membrane/metabolism , Laryngeal Neoplasms/metabolism , Adamantane/analogs & derivatives , Adamantane/chemistry , Aged , Carcinoma, Squamous Cell/pathology , Erythrocyte Membrane/chemistry , Humans , Laryngeal Neoplasms/pathology , Lipid Bilayers/chemistry , Lipid Bilayers/metabolism , Male , Membrane Fluidity , Membrane Lipids/chemistry , Membrane Lipids/metabolism , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Middle Aged , Molecular Probes/chemistry , Molecular Structure , Neoplasm Staging , Piperidines/chemistry , Time Factors , ViscosityABSTRACT
The experience of forming a preventive trend in the general practitioner (family doctor) approach in the context of the Bologna process in Europe has been studied. To stimulate a student's reason to apprehend hygienic approach in their future practice, written tests and tasks for the ongoing and interim control, which are similar to the tests of the final state exam on hygienehave been developed. These tests and tasks have been introduced in educational process.
