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1.
Cell Rep ; 25(5): 1135-1145.e5, 2018 10 30.
Article in English | MEDLINE | ID: mdl-30380406

ABSTRACT

RNase H2 has two distinct functions: initiation of the ribonucleotide excision repair (RER) pathway by cleaving ribonucleotides (rNMPs) incorporated during DNA replication and processing the RNA portion of an R-loop formed during transcription. An RNase H2 mutant lacking RER activity but supporting R-loop removal revealed that rNMPs in DNA initiate p53-dependent DNA damage response and early embryonic arrest in mouse. However, an RNase H2 AGS-related mutant with residual RER activity develops to birth. Estimations of the number of rNMPs in DNA in these two mutants define a ribonucleotide threshold above which p53 induces apoptosis. Below the threshold, rNMPs in DNA trigger an innate immune response. Compound heterozygous cells, containing both defective enzymes, retain rNMPs above the threshold, indicative of competition for RER substrates between active and inactive enzymes, suggesting that patients with compound heterozygous mutations in RNASEH2 genes may not reflect the properties of recombinantly expressed proteins.


Subject(s)
Embryonic Development , Mutation/genetics , Ribonuclease H/genetics , Ribonucleotides/metabolism , Animals , DNA/metabolism , DNA Damage , DNA Repair/drug effects , Embryo Loss/pathology , Embryo, Mammalian/abnormalities , Embryonic Development/drug effects , Fibroblasts/drug effects , Fibroblasts/metabolism , Interferons/pharmacology , Membrane Proteins/metabolism , Mice, Knockout , Mutant Proteins/metabolism , RNA Stability/drug effects , Ribonuclease H/metabolism , Tumor Suppressor Protein p53/metabolism
2.
Mol Biotechnol ; 54(2): 350-60, 2013 Jun.
Article in English | MEDLINE | ID: mdl-22706789

ABSTRACT

Adrenaline and noradrenaline are important neurotransmitter hormones that mediate physiological stress responses in adult mammals, and are essential for cardiovascular function during a critical period of embryonic/fetal development. In this study, we describe a novel mouse model system for identifying and characterizing adrenergic cells. Specifically, we generated a reporter mouse strain in which a nuclear-localized enhanced green fluorescent protein gene (nEGFP) was inserted into exon 1 of the gene encoding Phenylethanolamine n-methyltransferase (Pnmt), the enzyme responsible for production of adrenaline from noradrenaline. Our analysis demonstrates that this knock-in mutation effectively marks adrenergic cells in embryonic and adult mice. We see expression of nEGFP in Pnmt-expressing cells of the adrenal medulla in adult animals. We also note that nEGFP expression recapitulates the restricted expression of Pnmt in the embryonic heart. Finally, we show that nEGFP and Pnmt expressions are each induced in parallel during the in vitro differentiation of pluripotent mouse embryonic stem cells into beating cardiomyocytes. Thus, this new mouse genetic model should be useful for the identification and functional characterization of adrenergic cells in vitro and in vivo.


Subject(s)
Adrenal Medulla/metabolism , Genes, Reporter/genetics , Green Fluorescent Proteins/genetics , Adrenal Medulla/cytology , Animals , Embryonic Stem Cells/metabolism , Epinephrine/genetics , Epinephrine/metabolism , Gene Expression , Green Fluorescent Proteins/metabolism , Mice , Mutation , Myocytes, Cardiac/metabolism , Norepinephrine/genetics , Norepinephrine/metabolism , Phenylethanolamine N-Methyltransferase/genetics , Phenylethanolamine N-Methyltransferase/metabolism , Pluripotent Stem Cells/metabolism
3.
PLoS One ; 7(2): e31991, 2012.
Article in English | MEDLINE | ID: mdl-22384122

ABSTRACT

Pannexin1 (Panx1) forms large nonselective membrane channel that is implicated in paracrine and inflammatory signaling. In vitro experiments suggested that Panx1 could play a key role in ischemic death of hippocampal neurons. Since retinal ganglion cells (RGCs) express high levels of Panx1 and are susceptible to ischemic induced injury, we hypothesized that Panx1 contributes to rapid and selective loss of these neurons in ischemia. To test this hypothesis, we induced experimental retinal ischemia followed by reperfusion in live animals with the Panx1 channel genetically ablated either in the entire mouse (Panx1 KO), or only in neurons using the conditional knockout (Panx1 CKO) technology. Here we report that two distinct neurotoxic processes are induced in RGCs by ischemia in the wild type mice but are inactivated in Panx1KO and Panx1 CKO animals. First, the post-ischemic permeation of RGC plasma membranes is suppressed, as assessed by dye transfer and calcium imaging assays ex vivo and in vitro. Second, the inflammasome-mediated activation of caspase-1 and the production of interleukin-1ß in the Panx1 KO retinas are inhibited. Our findings indicate that post-ischemic neurotoxicity in the retina is mediated by previously uncharacterized pathways, which involve neuronal Panx1 and are intrinsic to RGCs. Thus, our work presents the in vivo evidence for neurotoxicity elicited by neuronal Panx1, and identifies this channel as a new therapeutic target in ischemic pathologies.


Subject(s)
Connexins/genetics , Ischemia/pathology , Nerve Tissue Proteins/genetics , Retinal Neurons/metabolism , Alleles , Animals , Calcium/metabolism , Connexins/metabolism , Glucose/chemistry , Hippocampus/metabolism , Inflammation , Interleukin-1beta/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Nerve Tissue Proteins/metabolism , Oxygen/chemistry , Reperfusion Injury , Retina/metabolism , Retinal Ganglion Cells/cytology
4.
Mol Cell Biol ; 30(5): 1108-15, 2010 Mar.
Article in English | MEDLINE | ID: mdl-20038532

ABSTRACT

The 2.4-kb H19 imprinting control region (H19ICR) is required to establish parent-of-origin-specific epigenetic marks and expression patterns at the Igf2/H19 locus. H19ICR activity is regulated by DNA methylation. The ICR is methylated in sperm but not in oocytes, and this paternal chromosome-specific methylation is maintained throughout development. We recently showed that the H19ICR can work as an ICR even when inserted into the normally nonimprinted alpha fetoprotein locus. Paternal but not maternal copies of the ICR become methylated in somatic tissue. However, the ectopic ICR remains unmethylated in sperm. To extend these findings and investigate the mechanisms that lead to methylation of the H19ICR in the male germ line, we characterized novel mouse knock-in lines. Our data confirm that the 2.4-kb element is an autonomously acting ICR whose function is not dependent on germ line methylation. Ectopic ICRs become methylated in the male germ line, but the timing of methylation is influenced by the insertion site and by additional genetic information. Our results support the idea that DNA methylation is not the primary genomic imprint and that the H19ICR insertion is sufficient to transmit parent-of-origin-dependent DNA methylation patterns independent of its methylation status in sperm.


Subject(s)
DNA Methylation , Genomic Imprinting , RNA, Untranslated/genetics , Spermatozoa/metabolism , Animals , Base Sequence , CD3 Complex/genetics , Chromosomes, Artificial, Bacterial/genetics , CpG Islands , DNA Primers/genetics , Female , Fertilization , Insulin-Like Growth Factor II/genetics , Male , Mice , Mice, Transgenic , Oocytes , Pregnancy , Promoter Regions, Genetic , RNA, Long Noncoding , Spermatogenesis/genetics , alpha-Fetoproteins/genetics
5.
J Clin Invest ; 119(8): 2317-29, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19662682

ABSTRACT

Psoriasis is a common immune-mediated chronic inflammatory skin disorder, but the mechanisms of pathogenesis are still poorly understood. IL-23 is expressed in psoriatic skin, and IL-23 injection produces IL-22-dependent psoriasiform changes in mouse skin. Th17 cells produce IL-22 and display CCR6, the CCL20 receptor; CCR6+ T cells and CCL20 are abundant in psoriatic skin. We investigated a possible role for CCR6 in recruiting Th17 cells and producing psoriasiform pathology by injecting IL-23 into the skin of WT and Ccr6-/- mice. Unlike for WT mice, IL-23-injected ears of Ccr6-/- mice showed neither substantial epidermal/dermal changes nor increased Il22 mRNA expression. However, injection of IL-22 yielded equivalent psoriasiform changes in WT and Ccr6-/- mice. Surprisingly, IL-23-injected ears of WT and Ccr6-/- mice contained similar numbers of Th cells able to make IL-17A and/or IL-22. Furthermore, in ears of Rag1-/- mice, IL-23 initially induced skin changes and levels of Il22 mRNA that were indistinguishable from WT mice, revealing at least one non-T cell source for IL-22. We conclude that CCR6 is essential in a model of IL-23-induced, IL-22-mediated dermatitis, which develops in sequential T cell-independent and T cell-dependent phases. These findings reveal an expanded role for CCR6 in IL-23-related responses and identify CCR6 as a potential therapeutic target in psoriasis.


Subject(s)
Interleukin-23/toxicity , Psoriasis/etiology , Receptors, CCR6/physiology , Animals , CD4-Positive T-Lymphocytes/physiology , Dendritic Cells/physiology , Homeodomain Proteins/physiology , Interleukin-17/biosynthesis , Interleukins/biosynthesis , Mice , Mice, Inbred C57BL , Interleukin-22
6.
Proc Natl Acad Sci U S A ; 104(32): 13182-6, 2007 Aug 07.
Article in English | MEDLINE | ID: mdl-17664423

ABSTRACT

Purkinje cells are one of the major types of neurons that form the neural circuitry in the cerebellum essential for fine control of movement and posture. During development, Purkinje cells also are critically involved in the regulation of proliferation of progenitors of granule cells, the other major type of neurons in the cerebellum. The process that controls differentiation of Purkinje cells from their early precursors is poorly understood. Here we report that two closely related LIM-homeobox genes, Lhx1 and Lhx5, were expressed in the developing Purkinje cells soon after they exited the cell cycle and migrated out of the cerebellar ventricular zone. Double-mutant mice lacking function of both Lhx1 and Lhx5 showed a severe reduction in the number of Purkinje cells. In addition, targeted inactivation of Ldb1, which encodes a cofactor for all LIM-homeodomain proteins, resulted in a similar phenotype. Our studies thus provide evidence that these transcription regulators are essential for controlling Purkinje cell differentiation in the developing mammalian cerebellum.


Subject(s)
Cerebellum/embryology , DNA-Binding Proteins/physiology , Homeodomain Proteins/physiology , Nerve Tissue Proteins/physiology , Purkinje Cells/cytology , Transcription Factors/physiology , Animals , Cell Differentiation , Female , LIM Domain Proteins , LIM-Homeodomain Proteins , Mice , Mice, Inbred C57BL , Pregnancy
7.
J Immunol ; 177(10): 6613-25, 2006 Nov 15.
Article in English | MEDLINE | ID: mdl-17082573

ABSTRACT

TCR signals drive thymocyte development, but it remains controversial what impact, if any, the intensity of those signals have on T cell differentiation in the thymus. In this study, we assess the impact of CD8 coreceptor signal strength on positive selection and CD4/CD8 lineage choice using novel gene knockin mice in which the endogenous CD8alpha gene has been re-engineered to encode the stronger signaling cytoplasmic tail of CD4, with the re-engineered CD8alpha gene referred to as CD8.4. We found that stronger signaling CD8.4 coreceptors specifically improved the efficiency of CD8-dependent positive selection and quantitatively increased the number of MHC class I (MHC-I)-specific thymocytes signaled to differentiate into CD8+ T cells, even for thymocytes expressing a single, transgenic TCR. Importantly, however, stronger signaling CD8.4 coreceptors did not alter the CD8 lineage choice of any MHC-I-specific thymocytes, even MHC-I-specific thymocytes expressing the high-affinity F5 transgenic TCR. This study documents in a physiologic in vivo model that coreceptor signal strength alters TCR-signaling thresholds for positive selection and so is a major determinant of the CD4:CD8 ratio, but it does not influence CD4/CD8 lineage choice.


Subject(s)
CD4 Antigens/physiology , CD4-Positive T-Lymphocytes/immunology , CD8 Antigens/physiology , CD8-Positive T-Lymphocytes/immunology , Cell Differentiation/immunology , Cell Lineage/immunology , Models, Immunological , Signal Transduction/immunology , Animals , CD4 Antigens/biosynthesis , CD4 Antigens/genetics , CD4-Positive T-Lymphocytes/cytology , CD4-Positive T-Lymphocytes/metabolism , CD8 Antigens/biosynthesis , CD8 Antigens/genetics , CD8-Positive T-Lymphocytes/cytology , CD8-Positive T-Lymphocytes/metabolism , Cell Differentiation/genetics , Cell Line , Cell Lineage/genetics , Female , Killer Cells, Natural/cytology , Killer Cells, Natural/immunology , Killer Cells, Natural/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Mutant Strains , Mice, Transgenic , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Signal Transduction/genetics , T-Lymphocytes, Helper-Inducer/cytology , T-Lymphocytes, Helper-Inducer/immunology , T-Lymphocytes, Helper-Inducer/metabolism , Up-Regulation/immunology
8.
Development ; 133(11): 2149-54, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16672341

ABSTRACT

The Dkk family of secreted cysteine-rich proteins regulates Wnt/beta-catenin signaling by interacting with the Wnt co-receptor Lrp5/6. Here, we show that Dkk2-mediated repression of the Wnt/beta-catenin pathway is essential to promote differentiation of the corneal epithelial progenitor cells into a non-keratinizing stratified epithelium. Complete transformation of the corneal epithelium into a stratified epithelium that expresses epidermal-specific differentiation markers and develops appendages such as hair follicles is achieved in the absence of the Dkk2 gene function. We show that Dkk2 is a key regulator of the corneal versus epidermal fate of the ocular surface epithelium.


Subject(s)
Cornea/growth & development , Cornea/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Animals , Biomarkers , Cornea/embryology , Epidermis/growth & development , Epidermis/metabolism , Epithelium/embryology , Epithelium/growth & development , Epithelium/metabolism , Female , Gene Expression Regulation, Developmental , Intercellular Signaling Peptides and Proteins/deficiency , Intercellular Signaling Peptides and Proteins/genetics , Keratinocytes/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Mutation/genetics , Phenotype
9.
Hum Mol Genet ; 15(6): 839-51, 2006 Mar 15.
Article in English | MEDLINE | ID: mdl-16446309

ABSTRACT

Smith-Lemli-Opitz syndrome (SLOS) is a genetic syndrome caused by mutations in the 3beta-hydroxysterol Delta(7)-reductase gene (DHCR7). SLOS patients have decreased cholesterol and increased 7-dehydrocholesterol (7-DHC) levels. Dietary cholesterol supplementation improves systemic biochemical abnormalities; however, because of the blood-brain barrier, the central nervous system (CNS) is not treated. Simvastatin therapy has been proposed as a means to treat the CNS. Mice homozygous for a null disruption of Dhcr7, Dhcr7(Delta3-5/Delta3-5), die soon after birth, thus they cannot be used to study postnatal development or therapy. To circumvent this problem, we produced a hypomorphic SLOS mouse model by introducing a mutation corresponding to DHCR7(T93M). Both Dhcr7(T93M/T93M) and Dhcr7(Delta3-5/T93M) mice are viable. Phenotypic findings in Dhcr7(T93M/Delta3-5) mice include CNS ventricular dilatation and two to three syndactyly. Biochemically, both Dhcr7(T93M/T93M) and Dhcr7(T93M/Delta3-5) mice have elevated tissue 7-DHC levels; however, the biochemical defect improved with age. This has not been observed in human patients, and is due to elevated Dhcr7 expression in mouse tissues. Dietary cholesterol therapy improved sterol profiles in peripheral, but not CNS tissues. However, treatment of Dhcr7(T93M/Delta3-5) mice with simvastatin decreased 7-DHC levels in both peripheral and brain tissues. Expression of Dhcr7 increased in Dhcr7(T93M/Delta3-5) tissues after simvastatin therapy, consistent with the hypothesis that simvastatin therapy improves the biochemical phenotype by increasing the expression of a Dhcr7 allele with residual enzymatic activity. We conclude that simvastatin treatment is efficacious in improving the SLOS-associated sterol abnormality found in the brain, and thus has the potential to be an effective therapeutic intervention for behavioral and learning problems associated with SLOS.


Subject(s)
Disease Models, Animal , Simvastatin/therapeutic use , Smith-Lemli-Opitz Syndrome/drug therapy , Smith-Lemli-Opitz Syndrome/genetics , 3T3 Cells , Alleles , Amino Acid Substitution/genetics , Animals , Cells, Cultured , Crosses, Genetic , Embryonic Stem Cells/transplantation , Female , Magnetic Resonance Spectroscopy , Methionine/genetics , Mice , Mice, Inbred C57BL , Mutagenesis, Site-Directed , Oxidoreductases Acting on CH-CH Group Donors/genetics , Phenotype , Smith-Lemli-Opitz Syndrome/enzymology , Threonine/genetics
10.
Mol Cell Biol ; 25(21): 9318-23, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16227583

ABSTRACT

The heterotetrameric adaptor protein (AP) complexes AP-1, AP-2, AP-3, and AP-4 play key roles in transport vesicle formation and cargo sorting in post-Golgi trafficking pathways. Studies on cultured mammalian cells have shown that AP-2 mediates rapid endocytosis of a subset of plasma membrane receptors. To determine whether this function is essential in the context of a whole mammalian organism, we carried out targeted disruption of the gene encoding the mu2 subunit of AP-2 in the mouse. We found that mu2 heterozygous mutant mice were viable and had an apparently normal phenotype. In contrast, no mu2 homozygous mutant embryos were identified among blastocysts from intercrossed heterozygotes, indicating that mu2-deficient embryos die before day 3.5 postcoitus (E3.5). These results indicate that AP-2 is indispensable for early embryonic development, which might be due to its requirement for cell viability.


Subject(s)
Adaptor Protein Complex 2/metabolism , Adaptor Protein Complex mu Subunits/metabolism , Adaptor Protein Complex 2/genetics , Adaptor Protein Complex mu Subunits/genetics , Animals , Cell Survival , Cells, Cultured , Embryo, Mammalian/cytology , Embryonic Development , Endocytosis , Fibroblasts/cytology , Fibroblasts/physiology , Heterozygote , Mice , Mice, Inbred C57BL , Mice, Knockout
11.
J Am Soc Nephrol ; 15(12): 3035-43, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15579506

ABSTRACT

Gene targeting has been used to create a variety of lines of mice with Pkd1 mutations that share many common features. Homozygous Pkd1 mutants invariably develop pancreatic and renal cysts if they survive to day 15.5 post coitum and die in either the fetal or the perinatal period. In contrast, mice with heterozygous mutations of Pkd1 are generally normal and have few if any renal cysts. These features have limited the utility of these models as tools to study the pathogenesis of cyst formation and the effect of various therapeutic interventions on disease progression. This report describes a new line of mice with a floxed allele of Pkd1 (Pkd1(cond)) that has an FRT-flanked neomycin cassette inserted into intron 1 and lox P sites inserted into intron 1 and intron 4. The Pkd1(cond) allele is fully functional, and homozygotes are viable and healthy. It is shown that the lox P and FRT sites can be selectively induced to recombine to produce two new alleles, Pkd1(del2-4) and Pkd1(cond-Deltaneo), by crossing to animals that express either the cre or FLPe recombinase, respectively. It is found that Pkd1(del2-4) allele functions as a true null, whereas presence or absence of the neomycin gene has no functional effects. It also is shown that somatic loss of Pkd1 results in renal and hepatic cysts. This new line of mice will be invaluable in the study of Pkd1 biology and serve as a powerful new tool that can be used to study the pathogenesis of autosomal dominant polycystic kidney disease.


Subject(s)
Mutagenesis, Insertional/methods , Polycystic Kidney, Autosomal Dominant/genetics , Polycystic Kidney, Autosomal Dominant/physiopathology , Proteins/genetics , Proteins/metabolism , Alleles , Animals , Disease Models, Animal , Kidney/pathology , Liver/pathology , Mice , Mice, Mutant Strains , Neomycin , Polycystic Kidney, Autosomal Dominant/pathology , TRPP Cation Channels
12.
Dev Dyn ; 231(4): 849-58, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15517585

ABSTRACT

To evaluate the developmental distribution of adrenergic cells in vivo, we inserted the Cre-recombinase gene into the locus encoding for the epinephrine biosynthetic enzyme phenylethanolamine n-methyltransferase (Pnmt) and crossed these Pnmt-Cre mice with ROSA26 reporter (R26R) mice to activate LacZ (encoding beta-galactosidase) expression in cells that were selectively derived from the adrenergic lineage. Our data show the following: (1) Insertion of Cre-recombinase into the Pnmt locus created a functional knockout of Pnmt expression with concomitant loss of epinephrine in homozygous Pnmt(Cre/Cre) mice; (2) Despite the reduction in Pnmt expression and epinephrine production in Pnmt(Cre/Cre) mice, these mice were viable and fertile, with no apparent developmental defects; (3) When crossed with R26R mice, Pnmt-Cre activation of LacZ expression faithfully recapitulated Pnmt expression in vivo; and (4) LacZ expression was activated in substantial numbers of pacemaking, conduction, and working cardiomyocytes.


Subject(s)
Adrenal Glands/embryology , Epinephrine/physiology , Integrases/genetics , Phenylethanolamine N-Methyltransferase/genetics , Adrenal Glands/cytology , Adrenal Glands/physiology , Animals , Female , Gene Expression Regulation, Developmental , Gene Expression Regulation, Enzymologic , Genes, Reporter , Lac Operon , Male , Mice , Mice, Knockout , Models, Animal , Mutagenesis, Insertional , Pregnancy
13.
Genomics ; 84(3): 555-64, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15498462

ABSTRACT

Inherited long QT syndrome is most frequently associated with mutations in KCNQ1, which encodes the primary subunit of a potassium channel. Patients with mutations in KCNQ1 may show only the cardiac defect (Romano-Ward syndrome or RWS) or may also have severe deafness (Jervell and Lange-Nielsen syndrome or JLNS). Targeted disruption of mouse Kcnq1 models JLNS in that mice are deaf and show abnormal ECGs. However, the phenotype is broader than that seen in patients. Most dramatically, the inner ear defects result in a severe hyperactivity/circling behavior, which may influence cardiac function. To understand the etiology of the cardiac phenotype in these mice and to generate a potentially more useful model system, we generated new mouse lines by introducing point mutations associated with RWS. The A340E line phenocopies RWS: the repolarization phenotype is inherited in a dominant manner and is observed independent of any inner ear defect. The T311I line phenocopies JLNS, with deafness associated with inner hair cell malfunction.


Subject(s)
Disease Models, Animal , Mice/genetics , Phenotype , Potassium Channels, Voltage-Gated/genetics , Romano-Ward Syndrome/genetics , Animals , Blotting, Northern , DNA Primers , Deafness/genetics , Electrocardiography , Evoked Potentials, Auditory, Brain Stem , Hair Cells, Auditory, Inner/pathology , KCNQ Potassium Channels , KCNQ1 Potassium Channel , Mutagenesis, Site-Directed
14.
Mol Cell Biol ; 24(9): 3588-95, 2004 May.
Article in English | MEDLINE | ID: mdl-15082756

ABSTRACT

Igf2 and H19 are coordinately regulated imprinted genes physically linked on the distal end of mouse chromosome 7. Genetic analyses demonstrate that the differentially methylated region (DMR) upstream of the H19 gene is necessary for three distinct functions: transcriptional insulation of the maternal Igf2 allele, transcriptional silencing of paternal H19 allele, and marking of the parental origin of the two chromosomes. To test the sufficiency of the DMR for the third function, we inserted DMR at two heterologous positions in the genome, downstream of H19 and at the alpha-fetoprotein locus on chromosome 5. Our results demonstrate that the DMR alone is sufficient to act as a mark of parental origin. Moreover, this activity is not dependent on germ line differences in DMR methylation. Thus, the DMR can mark its parental origin by a mechanism independent of its own DNA methylation.


Subject(s)
Chromosomes/metabolism , DNA Methylation , Genomic Imprinting , Proteins/genetics , RNA, Untranslated/genetics , Animals , Chromosomes/genetics , Female , Gene Expression Regulation, Developmental , Gene Silencing , Humans , Male , Mice , Protein Biosynthesis , Proteins/metabolism , RNA, Long Noncoding
15.
Proc Natl Acad Sci U S A ; 100(16): 9566-71, 2003 Aug 05.
Article in English | MEDLINE | ID: mdl-12886012

ABSTRACT

Neural progenitor cells are widespread throughout the adult central nervous system but only give rise to neurons in specific loci. Negative regulators of neurogenesis have therefore been postulated, but none have yet been identified as subserving a significant role in the adult brain. Here we report that nitric oxide (NO) acts as an important negative regulator of cell proliferation in the adult mammalian brain. We used two independent approaches to examine the function of NO in adult neurogenesis. In a pharmacological approach, we suppressed NO production in the rat brain by intraventricular infusion of an NO synthase inhibitor. In a genetic approach, we generated a null mutant neuronal NO synthase knockout mouse line by targeting the exon encoding active center of the enzyme. In both models, the number of new cells generated in neurogenic areas of the adult brain, the olfactory subependyma and the dentate gyrus, was strongly augmented, which indicates that division of neural stem cells in the adult brain is controlled by NO and suggests a strategy for enhancing neurogenesis in the adult central nervous system.


Subject(s)
Neurons/metabolism , Neurons/physiology , Nitric Oxide/metabolism , Animals , Brain/metabolism , Bromodeoxyuridine/pharmacology , Cell Division , Central Nervous System/metabolism , Exons , In Situ Nick-End Labeling , Mice , Mice, Knockout , Microscopy, Confocal , Models, Genetic , Molecular Sequence Data , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide Synthase/metabolism , Open Reading Frames , Rats , Recombination, Genetic
16.
Hum Mol Genet ; 12(13): 1631-41, 2003 Jul 01.
Article in English | MEDLINE | ID: mdl-12812989

ABSTRACT

Lathosterol 5-desaturase catalyzes the conversion of lathosterol to 7-dehydrocholesterol in the next to last step of cholesterol synthesis. Inborn errors of cholesterol synthesis underlie a group of human malformation syndromes including Smith-Lemli-Opitz syndrome, desmosterolosis, CHILD syndrome, CDPX2 and lathosterolosis. We disrupted the lathosterol 5-desaturase gene (Sc5d ) in order to further our understanding of the pathophysiological processes underlying these disorders and to gain insight into the corresponding human disorder. Sc5d (-/-) pups were stillborn, had elevated lathosterol and decreased cholesterol levels, had craniofacial defects including cleft palate and micrognathia, and limb patterning defects. Many of the malformations found in Sc5d (-/-) mice are consistent with impaired hedgehog signaling, and appear to be a result of decreased cholesterol rather than increased lathosterol. A patient initially described as atypical SLOS with mucolipidosis was shown to have lathosterolosis by biochemical and molecular analysis. We identified a homozygous mutation of SC5D (137A>C, Y46S) in this patient. An unique aspect of the lathosterolosis phenotype is the combination of a malformation syndrome with an intracellular storage defect.


Subject(s)
Lipid Metabolism, Inborn Errors/genetics , Oxidoreductases Acting on CH-CH Group Donors/deficiency , Oxidoreductases Acting on CH-CH Group Donors/genetics , Smith-Lemli-Opitz Syndrome/genetics , Amino Acid Sequence , Animals , Cholesterol/metabolism , Fibroblasts/metabolism , Fibroblasts/ultrastructure , Genotype , Homozygote , Humans , Infant, Newborn , Mice , Mice, Transgenic , Models, Chemical , Models, Genetic , Molecular Sequence Data , Mutation , Phenotype , Sequence Homology, Amino Acid , Skin/pathology , Sterols/metabolism , Time Factors
17.
Mol Cell ; 11(3): 807-15, 2003 Mar.
Article in English | MEDLINE | ID: mdl-12667461

ABSTRACT

Although ribonucleases H (RNases H) have long been implicated in DNA metabolism, they are not required for viability in prokaryotes or unicellular eukaryotes. We generated Rnaseh1(-/-) mice to investigate the role of RNase H1 in mammals and observed developmental arrest at E8.5 in null embryos. A fraction of the mainly nuclear RNase H1 was targeted to mitochondria, and its absence in embryos resulted in a significant decrease in mitochondrial DNA content, leading to apoptotic cell death. This report links RNase H1 to generation of mitochondrial DNA, providing direct support for the strand-coupled mechanism of mitochondrial DNA replication. These findings also have important implications for therapy of mitochondrial dysfunctions and drug development for the structurally related RNase H of HIV.


Subject(s)
DNA, Mitochondrial/physiology , Ribonuclease H/genetics , Ribonuclease H/physiology , Alleles , Amino Acid Sequence , Animals , Apoptosis , Blotting, Southern , Cell Nucleus/metabolism , DNA/biosynthesis , DNA/metabolism , Electron Transport , Genetic Vectors , Genotype , Homozygote , In Situ Nick-End Labeling , Mice , Mice, Inbred C57BL , Mice, Knockout , Models, Genetic , Molecular Sequence Data , Plasmids/metabolism , Polymerase Chain Reaction , Ribonuclease H/chemistry , Ribonuclease H/metabolism , Time Factors
18.
J Biol Chem ; 278(8): 5977-83, 2003 Feb 21.
Article in English | MEDLINE | ID: mdl-12270940

ABSTRACT

Understanding the molecular basis of monoallelic expression as observed at imprinted loci is helpful in understanding the mechanisms underlying epigenetic regulation. Genomic imprinting begins during gametogenesis with the establishment of epigenetic marks on the chromosomes such that paternal and maternal chromosomes are rendered distinct. During embryonic development, the primary imprint can lead to generation of secondary epigenetic modifications (secondary imprints) of the chromosomes. Eventually, either the primary imprints or the secondary imprints interfere with transcription, leading to parent-of-origin-dependent silencing of one of the two alleles. Here we investigated several aspects pertaining to the generation and functional necessity of secondary methylation imprints at the Igf2/H19 locus. At the H19 locus, these secondary imprints are, in fact, the signals mediating paternal chromosome-specific silencing of that gene. We first demonstrated that the H19 secondary methylation imprints are entirely stable through multiple cell divisions, even in the absence of the primary imprint. Second, we generated mouse mutations to determine which DNA sequences are important in mediating establishment and maintenance of the silent state of the paternal H19 allele. Finally, we analyzed the dependence of the methylation of Igf2DMR1 region on the primary methylation imprint about 90 kilobases away.


Subject(s)
Genomic Imprinting , Insulin-Like Growth Factor II/genetics , Proteins/genetics , Animals , Base Sequence , CD2 Antigens/genetics , Chromosome Mapping , DNA Methylation , DNA Primers , Embryonic and Fetal Development , Exons , Female , Gene Expression Regulation, Developmental , Humans , Lymphocyte Activation , Male , Mice , Mice, Knockout , Mice, Transgenic , Polymerase Chain Reaction , RNA, Long Noncoding , RNA, Untranslated/genetics , Restriction Mapping , T-Lymphocytes/immunology
19.
Development ; 130(3): 495-505, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12490556

ABSTRACT

The LIM domain-binding protein 1 (Ldb1) is found in multi-protein complexes containing various combinations of LIM-homeodomain, LIM-only, bHLH, GATA and Otx transcription factors. These proteins exert key functions during embryogenesis. Here we show that targeted deletion of the Ldb1 gene in mice results in a pleiotropic phenotype. There is no heart anlage and head structures are truncated anterior to the hindbrain. In about 40% of the mutants, posterior axis duplication is observed. There are also severe defects in mesoderm-derived extraembryonic structures, including the allantois, blood islands of the yolk sack, primordial germ cells and the amnion. Abnormal organizer gene expression during gastrulation may account for the observed axis defects in Ldb1 mutant embryos. The expression of several Wnt inhibitors is curtailed in the mutant, suggesting that Wnt pathways may be involved in axial patterning regulated by Ldb1.


Subject(s)
Body Patterning/genetics , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Gastrula/cytology , Zebrafish Proteins , Animals , Base Sequence , Body Patterning/physiology , DNA/genetics , DNA-Binding Proteins/physiology , Female , Fetal Heart/embryology , Gastrula/metabolism , Gene Targeting , LIM Domain Proteins , Male , Mesoderm/cytology , Mice , Mice, Knockout , Oocytes/metabolism , Phenotype , Pregnancy , Proto-Oncogene Proteins/antagonists & inhibitors , Proto-Oncogene Proteins/physiology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Wnt Proteins
20.
J Neurosci ; 22(24): 10801-10, 2002 Dec 15.
Article in English | MEDLINE | ID: mdl-12486173

ABSTRACT

Dopamine is an important transmitter in the CNS and PNS, critically regulating numerous neuropsychiatric and physiological functions. These actions of dopamine are mediated by five distinct receptor subtypes. Of these receptors, probably the least understood in terms of physiological functions is the D5 receptor subtype. To better understand the role of the D5 dopamine receptor (DAR) in normal physiology and behavior, we have now used gene-targeting technology to create mice that lack this receptor subtype. We find that the D5 receptor-deficient mice are viable and fertile and appear to develop normally. No compensatory alterations in other dopamine receptor subtypes were observed. We find, however, that the mutant mice develop hypertension and exhibit significantly elevated blood pressure (BP) by 3 months of age. This hypertension appears to be caused by increased sympathetic tone, primarily attributable to a CNS defect. Our data further suggest that this defect involves an oxytocin-dependent sensitization of V1 vasopressin and non-NMDA glutamatergic receptor-mediated pathways, potentially within the medulla, leading to increased sympathetic outflow. These results indicate that D5 dopamine receptors modulate neuronal pathways regulating blood pressure responses and may provide new insights into mechanisms for some forms of essential hypertension in humans, a disease that afflicts up to 25% of the aged adult population in industrialized societies.


Subject(s)
Hypertension/etiology , Receptors, Dopamine D1/physiology , Sympathetic Nervous System/physiopathology , Adrenal Glands/chemistry , Animals , Blood Pressure , Brain/metabolism , Brain Chemistry , Epinephrine/analysis , Gene Targeting , Hypertension/metabolism , Hypertension/physiopathology , Immunohistochemistry , Mice , Mice, Inbred C57BL , Mice, Knockout , Norepinephrine/analysis , Oxytocin/genetics , RNA, Messenger/analysis , Receptors, Dopamine D1/analysis , Receptors, Dopamine D1/genetics , Receptors, Dopamine D2/metabolism , Receptors, Dopamine D5 , Receptors, Oxytocin/analysis , Receptors, Vasopressin/analysis , Reverse Transcriptase Polymerase Chain Reaction , Vasopressins/genetics
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