[Effect of preparations of unsaturated fatty acids on the lipid component of the endoplasmic reticulum membranes of liver cells in rats following administration of N-nitrosodiethylamine]. / Vliianie preparatov nenasyshchennykh zhirnykh kislot na lipidnyi komponent membran éndoplazmaticheskogo retikuluma kletok pecheni krys pri vvedenii N-nitrozodiétilamina.

The effect of drugs, including unsaturated fatty acids and antioxidants, on the lipid composition of the endoplasmic reticulum membranes was studied at early stages of NDEA-induced hepatocarcinogenesis. The complexes containing oleic, linoleic, and arachidonic acids and biological antioxidants prevent changes in the fatty acid composition and the lipid bilayer organization of the both membrane fractions of endoplasmic reticulum which are observed after the injection of NDEA alone, and they inhibit the appearance and growth in the liver of gamma-glutamyltranspeptidase positive hyperplastic nodules.

[Molecular electron carriers in mouse tissues during development of sarcomas induced by 3-methylcholanthrene and Moloney virus]. / Molekuliarnye perenoschiki élektronov v tkaniakh myshei pri razvitii sarkom, indutsirovannykh 3-metilkholantrenom i virusom Moloni.

Regular changes are revealed in electron-transport chains of the energy and detoxifying systems. The changes occur mainly in iron-sulphur centres and cytochrome P-450 under chemical and viral blastomogenesis in the muscular tissue induced by 3-methyl cholanthrene and Moloney oncornavirus. In the tissue of developing Moloney tumour and spleen kinetics of the formation of triplet signal of nitrosyl complexes is recorded in the region of g-factor of free radicals of semiquinone type and its transformation into singlet signal of EPR with the Moloney sarcoma regression.

[Fatty acid content in the muscles and liver of mice developing sarcomas induced by 3-methylcholanthrene and Moloney murine sarcoma virus]. / Soderzhanie zhirnykh kislot v myshtsakh i pecheni myshei pri razvitii sarkom, indutsirovannykh 3-metilkholantrenom i virusom sarkomy myshei Moloni.

A comparative analysis was made for the fatty acid composition of lipids in the malignant muscular tissue, muscle and liver under blastomogenesis induced in mice by the Moloney sarcoma virus and 3-methylcholanthrene. The relation of the found changes in the fatty acid composition to the blastomogenesis stages is shown. The development of the tumour node is accompanied by a decrease of the fatty acid amount in the animal tissues and by its increase in the tumour. A spontaneous regression of the Moloney sarcoma results in increased level of the fatty acid content in the animal tissues followed by its normalization.

[Effect of modification of the lipid bilayer on the functioning of the electron transport chain of endoplasmic reticulum membranes in nitrosodiethylamine-induced hepatic carcinogenesis]. / Vliianie modifikatsii lipidnogo bisloia na funktsionirovanie élektrontransportnoi tsepi membran éndoplazmaticheskogo retikuluma pri gepatokantserogeneze, indutsirovannom nitrozodiétilaminom.

The kinetics of cytochrome P-450 content in endoplasmic reticulum membranes at different stages of hepatocarcinogenesis was studied under the influence of unsaturated fatty acids by the EPR method. At early stages of carcinogenesis the preparations containing oleic, linoleic and arachidonic acids prevent changes in the cytochrome P-450 content usually observed in case of nitrosodiethylamine introduction. The diet containing unsaturated fatty acids has a stabilizing effect revealed by the similar changes in the content of cytochrome P-450, phosphatidyl choline and fatty acids composition and also in the level of organization of the endoplasmic reticulum membrane lipid bilayer.

[Paramagnetic centers during carcinognesis induced by an RNA-containing virus]. / Paramagnitnye tsentry pri kantserogeneze, indutsirovannom RNK-soderzhashchim virusom.

An ESR method was used to investigate the metalloenzyme centres, characteristics of cell energetic and detoxicity systems of models of tumors induced by RNA virus MSV(M) as progressor and regressor of tumor development. Different stages of ESR signal formation and its reverse transformation centres that reflect relations between tumor and organism the iron--sulphur proteins proved to be the most important.

[Oxidative free radical processes and bioantioxidants during viral carcinogenesis]. / Okysni vil'noradykal'ni protsesy ta bioantyoksydanty za virusnoho kantserohenezu

The changes in biochemiluminescence intensity of tissue homogenates, tissue lipids as well as the values of the antioxidative activity of the free-radical processes regulators, bioantioxidants of lipids, and their antiradical activity were studied in viral carcinogenesis induced by Moloney's oncornavirus. Kinetics of biochemiluminescence of muscles homogenates and muscular tissue tumours (Moloney's sarcoma) and that of chemiluminescence of total lipids extracted from the muscular and tumour tissue were studied as well. A sharp increase in the intensity of lipids biochemiluminiscence and decrease in the tissue homogenates biochemiluminiscence were observed during the period of progressive tumour growth on the 6-8 days following introduction of the virus. At the same time the values of the antioxidative activity and antiradical activity of the tissue lipids bioantioxidants were minimal. Results of this investigation suggest that the expenditure of bioantioxidants are rather active during the period of progressive tumour growth. The regularities of the changes in biochemiluminescence intensity in the muscle tissue homogenates with viral carcinogenesis cannot be explained exceptionally by an increase in the amount of bioantioxidants in the tumour tissue lipids. Evidently the peculiarities of lipid-protein interactions might play an essential role in the mentioned process.