ABSTRACT
Chronic post-traumatic osteomyelitis was mainly caused (59.3 per cent) by various gramnegative bacteria. Staphylococcus, Proteus and P. aeruginosa was most frequent. Bacteria were most frequently isolated from associations of 2-5 species and not from monocultures. However, when the patient microflora was studied in dynamics, the whole associations could not be detected in every of the cases. Sensitivity of the causative agents to 23 antibacterial drugs was tested and it was found that the majority of the organisms were sensitive to gentamicin, rifampicin, biseptol, hydroxyquinolines (5-NOK, enteroseptol), furazolidon (except P. aeruginosa). P. aeruginosa was in addition sensitive to polymyxin. Proteus and other enterobacteria were sensitive to nevigramon, staphylococci and other grampositive bacteria were sensitive to oxacillin, lincomycin, novobiocin and fusidin.
Subject(s)
Bacteria/drug effects , Osteomyelitis/microbiology , Bacteria/isolation & purification , Chronic Disease , Drug Resistance, Microbial , Humans , Microbial Sensitivity TestsABSTRACT
The studies provided development of a new method consisting of placing 2 filter paper strips each impregnated with an antibiotic (2 antibiotics are used out of 3: levomycetin, tetracycline and streptomycin) under a layer of Ploskirev's medium with sodium sulfacyl. The antibiotics are chosen proceeding from the predominance in the given area of Shigella resistant to some of the above antibiotics. The method simplifies and makes more economic the tests, provides higher frequency of Shigella isolation as compared to the procedure with the routine Ploskirev's medium or gradient dishes, has almost the same isolation frequency as with the use of 2 plates (with and without antibiotic). The method is recommended for wide use in practice.
Subject(s)
Chloramphenicol , Dysentery, Bacillary/diagnosis , Streptomycin , Tetracycline , Bacteriological Techniques , Culture Media , Feces/microbiology , Humans , Paper , Shigella/isolation & purificationABSTRACT
A new variant of media with antibiotics for isolation of Shigella, i.e. a method of 2 streaks each containing different antibiotics was tested in analysis of excrements from patients with acute dysentery. It was found that the new method is more effective than the well known method of gradient plates (isolation of Shigella in one series of the experiments amounted to 85.2 and 64.7 per cent respectively, and in the other series of the experiments the respective figures were 95.4 and 89.3 per cent). Its efficiency was lower as compared to the procedure of inoculation onto 2 plates, i.e. onto the media with and without an antibiotic (isolation of Shigella was 67.5 and 77.4 per cent respectively). The new method provided a higher frequency of Shigella isolation as compared to inoculation onto the media without an antibiotic, as well as onto any of the media used with one antibiotic. The method of 2 streaks offers wider possibilities for choosing the antibiotics for adding to the nutrient medium, as well as estimation of the antibioticograms and species structures of Shigella distributed in a concrete area.