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1.
Acta Neurol Belg ; 111(3): 175-82, 2011 Sep.
Article in English | MEDLINE | ID: mdl-22141279

ABSTRACT

This paper proposes therapeutic guidelines for the management of some epileptic syndromes in infants, children, and adolescents, based on available medical literature and clinical practice in the French Community of Belgium. The guidelines address both epileptic encephalopathies (West syndrome, Lennox-Gastaut syndrome, and Dravet syndrome) and idiopathic epilepsies (typical absence seizures, epilepsy with centro-temporal spikes and juvenile myoclonic epilepsy).


Subject(s)
Anticonvulsants/therapeutic use , Epilepsy/drug therapy , Adolescent , Age Factors , Child , Humans , Infant , Intellectual Disability/drug therapy , Lennox Gastaut Syndrome , Myoclonic Epilepsy, Juvenile/drug therapy , Spasms, Infantile/drug therapy
2.
Rev Med Liege ; 65(5-6): 366-9, 2010.
Article in French | MEDLINE | ID: mdl-20684421

ABSTRACT

Epilepsy is a chronic disease, requiring a medical treatment which is essentially preventive (avoiding further seizure). Because of these characteristics, 30 to 50% of epileptic patients do not always comply with their treatment. In this paper, we review the different factors of poor compliance. Some are specific to this medical condition, while others are more general, like treatment complexity. We list some suggestions to improve the compliance of the epileptic patient in routine medical practice.


Subject(s)
Epilepsy/drug therapy , Medication Adherence , Humans
3.
Rev Med Liege ; 63(5-6): 303-9, 2008.
Article in French | MEDLINE | ID: mdl-18669197

ABSTRACT

The epilepsies represent different neurological disorders in terms of aetiology, prognosis and treatment, sharing the occurrence of clinical seizures of various clinical pictures following the development of abnormal hypersynchrone electrical discharges from the brain cortical mantle. It becomes therefore crucial to first recognize the epileptic nature of any paroxystic or sudden clinical event and, further, to establish the syndromic diagnosis in order to propose the most adequate treatment and prognosis. Important advances in functional brain imaging technologies and also in the description of seizures semiology using video-EEG have permitted to cure certain epileptic syndromes by surgery. On the other hand, the persistence of a set of pharmaco-resistant and incurable epileptic syndromes must encourage the pursuit of basic researches, including new therapeutic pharmacological approaches as well as the use of techniques using brain neurostimulation.


Subject(s)
Epilepsy/diagnosis , Epilepsy/therapy , Decision Trees , Humans
4.
Acta Neurol Belg ; 106(3): 111-6, 2006 Sep.
Article in English | MEDLINE | ID: mdl-17091613

ABSTRACT

Seizures starting in patients over 60 years old are frequent. Diagnosis is sometimes difficult and frequently under- or overrated. Cerebrovascular disorders are the main cause of a first seizure. Because of more frequent comorbidities, physiologic changes, and a higher sensitivity to drugs, treatment has some specificity in elderly people. The aim of this paper is to present the result of a consensus meeting held in October 2004 by a Belgian French-speaking group of epileptologists and to propose guidelines for the management and the treatment of epilepsy in elderly people.


Subject(s)
Anticonvulsants/therapeutic use , Epilepsy/diagnosis , Epilepsy/drug therapy , Aged , Algorithms , Brain/drug effects , Brain/physiopathology , Humans
5.
Acta Neurol Belg ; 106(2): 52-60, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16898254

ABSTRACT

The authors propose to define the epileptic syndromes with continuous spikes and waves during slow sleep (CSWS) as a cognitive or behavioral impairment acquired during childhood, associated with a strong activation of the interictal epileptiform discharges during NREM sleep--whatever focal or generalized--and not related to another factor than the presence of CSWS. The type of syndrome will be defined according to the neurological and neuropsychological deficit. These syndromes have to be classified among the localization-related epileptic syndromes. Some cases are idiopathic and others are symptomatic. Guidelines for work-up and treatment are proposed.


Subject(s)
Action Potentials/physiology , Epilepsy/physiopathology , Epilepsy/therapy , Practice Guidelines as Topic/standards , Sleep/physiology , Humans , Syndrome
6.
Neurophysiol Clin ; 36(5-6): 271-9, 2006.
Article in English | MEDLINE | ID: mdl-17336771

ABSTRACT

Can a gene defect be responsible for the occurrence in an individual, at a particular age, of such a muscle twitch followed by relaxation called: "myoclonus" and defined as sudden, brief, shock-like movements? Genetic defects could indeed determine a subsequent cascade of molecular events (caused by abnormal encoded proteins) that would produce new aberrant cellular relationships in a particular area of the CNS leading to re-built "myoclonogenic" neuronal networks. This can be illustrated reviewing some inherited neurological entities that are characterized by a predominant myoclonic picture and among which a clear gene defect has been identified. In the second part of this chapter, we will also propose a new point of view on how some structural genes could, under certain conditions, when altered, produced idiopathic generalized epilepsy with myoclonic jerks, taking juvenile myoclonic epilepsy (JME) and the myoclonin (EFHC-1) gene as examples.


Subject(s)
Calcium-Binding Proteins/physiology , Myoclonus/genetics , Adult , Animals , Brain/embryology , Brain/metabolism , COS Cells , Calcium-Binding Proteins/deficiency , Calcium-Binding Proteins/genetics , Cell Line , Child , Chlorocebus aethiops , Cricetinae , Disease Progression , Epilepsies, Myoclonic/classification , Epilepsies, Myoclonic/genetics , Epilepsies, Myoclonic/physiopathology , Genetic Diseases, Inborn/complications , Genetic Diseases, Inborn/genetics , HeLa Cells , Humans , Mesocricetus , Mice , Myoclonic Epilepsy, Juvenile/genetics , Myoclonus/classification , Myoclonus/etiology , Myoclonus/metabolism , Nervous System Diseases/complications , Nervous System Diseases/genetics , Nervous System Diseases/physiopathology , Neurons/metabolism , Spindle Apparatus/metabolism , Syndrome , Transfection
7.
Acta Neurol Belg ; 105(1): 5-13, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15861989

ABSTRACT

In many circumstances antiepileptic drugs are used in patients who have never presented any clinical epileptic seizures. These substances are administered on the assumption of a potential risk for the patients of developing acute or delayed chronic seizures after brain injuries such as trauma, stroke, hemorrages or even neurosurgical interventions. The aim of this paper is to propose therapeutic guidelines for the management of this prophylactic attitude in epilepsy based on basic research and clinical practice in the French community in Belgium. We will distinguish between the prevention of acute (early onset-provoked) seizures and a delayed truly post-lesional (unprovoked) epilepsy. Some therapeutic goals can be achieved under the former circumstances whereas in the latter situation we all agree for the absence of any coherent antiepileptic prophylactic behaviour.


Subject(s)
Anticonvulsants/therapeutic use , Brain Injuries/drug therapy , Epilepsy/drug therapy , Epilepsy/prevention & control , Acute Disease , Brain Injuries/epidemiology , Epilepsy/epidemiology , Humans , Risk Factors
8.
Acta Neurol Belg ; 105(1): 14-7, 2005 Mar.
Article in English | MEDLINE | ID: mdl-15861990

ABSTRACT

Epilepsy and psychiatric diseases are frequent comorbidities. Psychoses in patients with epilepsy have special physiopathology and several clinical presentations and prognoses. Their treatments are also specific, according to the specific diagnosis. This paper represents the summary of a consensus meeting held in November 2003 by a Belgian French-speaking group of neurologists, neuropediatricians and psychiatrists and proposes guidelines for the recognition and treatment of those entities.


Subject(s)
Epilepsy/complications , Practice Guidelines as Topic , Psychotic Disorders/diagnosis , Psychotic Disorders/etiology , Antipsychotic Agents/therapeutic use , Humans , Psychotic Disorders/drug therapy
9.
Neurology ; 62(12): 2214-20, 2004 Jun 22.
Article in English | MEDLINE | ID: mdl-15210885

ABSTRACT

INTRODUCTION: Posterior quadrantic dysplasia (PQD), a developmental malformation involving the temporal, parietal, and occipital lobes of one cerebral hemisphere, leads to intractable epilepsy. OBJECTIVE: To characterize the clinical features of 19 patients with PQD and analyze the postsurgical outcome of those who underwent resection of dysplastic tissue. METHODS: The extent and nature of the malformation were primarily assessed with high-resolution brain imaging. Fourteen patients underwent complete or partial temporoparieto-occipital resection or temporal resection associated with parieto-occipital disconnection. Postoperative follow-up period ranged from 8 months to 7 years. The authors used the Engel classification for postoperative outcome. RESULTS: All patients were sporadic. Clinical features included infantile spasms, partial seizures, mental retardation, mild hemiparesis, and visual field defects. Neuroimaging localized the malformation within the posterior cerebral quadrant contralateral to the neurologic deficit and demonstrated hemi-hemimegalencephaly in 14 of 19 patients and multilobar cortical dysplasia in 5 of 19 patients. The authors observed class I outcome in six patients. Two patients had class II and four patients had class III outcome. Class IV outcome was seen in two patients. After surgery, two patients developed mild hemiparesis, and two developed a visual field defect. CONCLUSIONS: Widespread cortical dysplasia is more frequent in the posterior quadrant. In our series, posterior quadrantic dysplasia represents either hemi-hemimegalencephaly or multilobar cortical dysplasia. Individuals with posterior quadrantic dysplasia share a spectrum of clinical features. The intractable epilepsy in these patients may be alleviated by a large quadrantic temporoparieto-occipital resection.


Subject(s)
Cerebral Cortex/abnormalities , Cerebral Cortex/surgery , Epilepsies, Partial/surgery , Adolescent , Age of Onset , Child , Child, Preschool , Electroencephalography , Epilepsies, Partial/etiology , Epilepsies, Partial/pathology , Female , Hemispherectomy , Humans , Infant , Male , Treatment Outcome
10.
Neuroscience ; 125(4): 833-40, 2004.
Article in English | MEDLINE | ID: mdl-15120844

ABSTRACT

Thiamine triphosphate (ThTP) is found in small amounts in most organisms from bacteria to mammals, but little is known about its physiological role. In vertebrate tissues, ThTP may act as a phosphate donor for the phosphorylation of certain proteins; this may be part of a new signal transduction pathway. We have recently characterized a highly specific 25-kDa thiamine triphosphatase (ThTPase) that is expressed in most mammalian tissues. The role of this enzyme may be the control of intracellular concentrations of ThTP. As the latter has been considered to be a neuroactive form of thiamine, we have studied the distribution of ThTPase mRNA and protein in rodent brain using in situ hybridization and immunohistochemistry. With both methods, we found the strongest staining in hippocampal pyramidal neurons, as well as cerebellar granule cells and Purkinje cells. Some interneurons were also labeled and many ThTPase mRNA-positive and immunoreactive cells were distributed throughout cerebral cortical gray matter and the thalamus. White matter was not significantly labeled. ThTPase immunoreactivity seems to be located mainly in the cytoplasm of neuronal perikarya. Immunocytochemical data using dissociated cultured cells from hippocampal and cerebellum showed that the staining was more intense in neurons than in astrocytes. The protein was rather uniformly located in the perikarya and dendrites, suggesting that ThTP and ThTPase may play a general role in neuronal metabolism rather than a specific role in excitability. There was no apparent correlation between ThTPase expression and selective vulnerability of certain brain regions to thiamine deficiency.


Subject(s)
Brain/enzymology , Neurons/enzymology , Thiamin-Triphosphatase/metabolism , Animals , Humans , Immunohistochemistry , In Situ Hybridization , Male , Mice , RNA, Messenger/analysis , Rats , Rats, Wistar , Recombinant Proteins/metabolism
11.
Acta Neurol Belg ; 103(3): 135-9, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14626692

ABSTRACT

Approximately 20% of people with epilepsy are of childbearing potential and about 3 to 5 births per thousand will be to women with epilepsy. Both epilepsy and antiepileptic drugs can cause specific problems in women and embryos (less than 8 weeks of gestational age) or foetuses (more than 8 weeks of gestational age). The aim of this paper is to discuss therapeutic issues for the management of women with epilepsy: initiation of antiepileptic therapy, contraception, pregnancy, breast feeding and menopause. Some fertility issues are also discussed.


Subject(s)
Anticonvulsants/therapeutic use , Epilepsy/drug therapy , Anticonvulsants/adverse effects , Breast Feeding , Female , Fertility/drug effects , Fertility/physiology , Humans , Menopause/physiology , Pregnancy/drug effects , Pregnancy/physiology
12.
Cell Mol Life Sci ; 60(7): 1477-88, 2003 Jul.
Article in English | MEDLINE | ID: mdl-12943234

ABSTRACT

In most organisms, the main form of thiamine is the coenzyme thiamine diphosphate. Thiamine triphosphate (ThTP) is also found in low amounts in most vertebrate tissues and can phosphorylate certain proteins. Here we show that ThTP exists not only in vertebrates but is present in bacteria, fungi, plants and invertebrates. Unexpectedly, we found that in Escherichia coli as well as in Arabidopsis thaliana, ThTP was synthesized only under particular circumstances such as hypoxia (E. coli) or withering (A. thaliana). In mammalian tissues, ThTP concentrations are regulated by a specific thiamine triphosphatase that we have recently characterized. This enzyme was found only in mammals. In other organisms, ThTP can be hydrolyzed by unspecific phosphohydrolases. The occurrence of ThTP from prokaryotes to mammals suggests that it may have a basic role in cell metabolism or cell signaling. A decreased content may contribute to the symptoms observed during thiamine deficiency.


Subject(s)
Bacteria/metabolism , Thiamin-Triphosphatase/metabolism , Thiamine Triphosphate/metabolism , Amino Acid Sequence , Animals , Bacteria/enzymology , Brain/enzymology , Cattle , Fungi/enzymology , Fungi/metabolism , Humans , Invertebrates , Male , Mammals , Molecular Sequence Data , Plants/enzymology , Plants/metabolism , Recombinant Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Species Specificity , Swine , Thiamin-Triphosphatase/chemistry
13.
Neural Comput ; 12(7): 1553-71, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10935918

ABSTRACT

A computer model of a thalamic network was used in order to examine the effects of an isolated augmentation in a low-threshold calcium current. Such an isolated augmentation has been observed in the reticular thalamic (RE) nucleus of the genetic absence epilepsy rat from the Strasbourg (GAERS) model of absence epilepsy. An augmentation of the low-threshold calcium conductance in the RE neurons (gTs) of the model thalamic network was found to lead to an increase in the synchronized firing of the network. This supports the hypothesis that the isolated increase in gTs may be responsible for epileptic activity in the GAERS rat. The increase of gTs in the RE neurons led to a slight increase in the period of the isolated RE neuron firing. In contrast, the low-threshold spike of the RE neuron remained relatively unchanged by the increase of gTs. This suggests that the enhanced synchrony in the network was primarily due to a phase shift in the firing of the RE neurons with respect to the thalamocortical neurons. The ability of this phase-shift mechanism to lead to changes in synchrony was further examined using the model thalamic network. A similar increase in the period of RE neuron oscillations was obtained through an increase in the conductance of the calcium-mediated potassium channel. This change was once again found to increase synchronous firing in the network.


Subject(s)
Calcium/metabolism , Epilepsy, Absence/physiopathology , Models, Neurological , Neurons/physiology , Thalamic Nuclei/physiology , Action Potentials/physiology , Animals , Computer Simulation , Electric Conductivity , Potassium/metabolism , Potassium Channels/physiology , Rats , Rats, Mutant Strains , Sodium/metabolism , Synapses/physiology , Thalamic Nuclei/cytology
14.
Connect Tissue Res ; 41(1): 69-80, 2000.
Article in English | MEDLINE | ID: mdl-10826710

ABSTRACT

Tobacco smoking is considered a major risk factor for the development and progression of periodontal diseases (Haber, J. and Wattles, J. (1994). J. Periodontol., 64, 16-23). The purpose of this study was to determine the effects of nicotine on rat gingival fibroblasts (RGF) cultured in vitro. After ether anesthesia, rat gingival tissues were obtained from the attached gingiva of a Wistar rat. Small fragments of gingiva were maintained in culture in Petri dishes. Fibroblasts developing from these explants were collected to obtain monolayer cultures. After the fourth passage (T4), cells were supplemented with nicotine at various concentrations. Control and treated cells were examined under phase contrast or transmission electron microscopy. They were compared as regards their DNA content, mitochondrial activity, collagen and protein synthesis, and cell death by apoptosis or necrosis. Nicotine from 0.05 microM to 1 mM did not affect the DNA content or protein and collagen synthesis. At concentrations between 3 and 5 mM, growth was significantly diminished and the survival rate reduced. Ultrastructural analysis revealed dilated mitochondria and vacuolization in treated cells, suggestive of necrosis, but increased apoptosis was also revealed by cytometry. On the basis of this in vitro study, it appears that tobacco, through its component nicotine, may directly affect various functions of RGF.


Subject(s)
Fibroblasts/drug effects , Gingiva/drug effects , Nicotine/pharmacology , Animals , Apoptosis , Cell Division/drug effects , Cells, Cultured , Collagen/biosynthesis , DNA/analysis , Fibroblasts/metabolism , Fibroblasts/ultrastructure , Gingiva/metabolism , Gingiva/ultrastructure , Male , Mitochondria/metabolism , Mitochondria/ultrastructure , Necrosis , Nicotine/administration & dosage , Protein Biosynthesis , Rats , Rats, Wistar , Succinate Dehydrogenase/metabolism , Vacuoles/ultrastructure
15.
Exp Neurol ; 162(1): 112-20, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10716893

ABSTRACT

Differential mRNA display was carried out to find genes that are differentially regulated in the brain of a rat strain with absence epilepsy, the genetic absence epilepsy rats from Strasbourg (GAERS). Among the 32 differentially displayed cDNA fragments actually cloned and sequenced, one shows 100% identity with the rat heavy chain ferritin (H-ferritin) mRNA. Northern blot analysis confirmed the up-regulation of the H-ferritin mRNA. Using dot blotting, a 40% increase in expression was reported in the subcortical forebrain of the adult GAERS, while cortex, brain stem, and cerebellum appeared unmodified. This change was not observed in the brain of 25-day-old rats, an age at which the epileptic phenotype is not present. By in situ hybridization, the enhanced expression was localized in the hippocampus. The increase in mRNA encoding H-ferritin was not immunodetected at the protein level by Western blotting. These results are not apparently related to the neural substrate of SWD or to the distribution of local increase in glucose metabolism previously described in the GAERS. It is hypothesized that the up-regulation of the H-ferritin mRNA is part of a mechanism protecting the hippocampus, a seizure-prone area, against a possible overactivation during absence seizures.


Subject(s)
Brain Chemistry/genetics , Epilepsy, Absence/genetics , Ferritins/genetics , Age Factors , Amino Acid Sequence , Animals , Blotting, Northern , Blotting, Western , Disease Models, Animal , Epilepsy, Absence/physiopathology , Ferritins/analysis , Gene Expression Regulation, Developmental/physiology , Hippocampus/chemistry , Hippocampus/physiology , In Situ Hybridization , Kindling, Neurologic/physiology , Male , Molecular Sequence Data , Phenotype , RNA, Messenger/metabolism , Rats , Rats, Mutant Strains , Rats, Wistar , Transcription, Genetic/physiology
17.
J Periodontol ; 70(10): 1234-9, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10534079

ABSTRACT

BACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery. METHODS: Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours. RESULTS: When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used. CONCLUSIONS: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR.


Subject(s)
Absorbable Implants , Cell Culture Techniques/methods , Culture Media , Gingiva/cytology , Acid Phosphatase/metabolism , Animals , Cells, Cultured , DNA/biosynthesis , Fibroblasts/cytology , Fibroblasts/metabolism , Gingiva/metabolism , Guided Tissue Regeneration, Periodontal , Male , Membranes, Artificial , Microscopy, Phase-Contrast , Rats , Rats, Wistar
18.
J Neurobiol ; 40(3): 327-42, 1999 Sep 05.
Article in English | MEDLINE | ID: mdl-10440733

ABSTRACT

A partial estrogen receptor-beta (ERbeta) cDNA had been previously cloned and sequenced in Japanese quail. The 3'- and 5'-rapid amplification of cDNA ends techniques were used here to identify a cDNA sequence of the quail ERbeta that contains a complete open reading frame. For the first time in an avian species, this cDNA sequence and the corresponding amino acid sequence are described. They are compared with the known ERbeta sequences previously described in mammals and with the ERalpha sequences identified in a selection of mammalian and avian species. The analysis by Northern blotting of the ERbeta mRNA expression in the brain and kidneys revealed the presence of several transcripts. The presence of ERbeta identified by reverse transcriptase-polymerase chain reaction demonstrated a widespread distribution quite different from the distribution of ERalpha. The complete neuroanatomical distribution of ERbeta mRNA as determined by in situ hybridization with 35S- and 33P-labeled oligoprobes is also presented. Transcripts are present in many nuclei implicated in the control of reproduction such as the medial preoptic nucleus, the nucleus striae terminalis, and the nucleus taeniae, the avian homologue of the amygdala. These data demonstrate the presence of ERbeta in a nonmammalian species and indicate that the (neuro)-anatomical distribution of this receptor type has been conserved in these two classes of vertebrates. The role of this receptor in the control of reproduction and other physiological processes should now be investigated.


Subject(s)
Cloning, Molecular/methods , Coturnix/genetics , Hypothalamus/anatomy & histology , Neurons/physiology , Receptors, Estrogen/genetics , Animals , Autoradiography/methods , Base Sequence , Blotting, Northern , Brain Chemistry/physiology , Cell Count , DNA Primers/genetics , DNA, Complementary/genetics , Glyceraldehyde-3-Phosphate Dehydrogenases/genetics , In Situ Hybridization , Male , Molecular Sequence Data , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction/methods , Tissue Distribution , Transcription, Genetic
19.
Neuroreport ; 10(3): 569-74, 1999 Feb 25.
Article in English | MEDLINE | ID: mdl-10208591

ABSTRACT

Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding calcium channel alpha1E and alpha1G subunits was measured in the brain of genetic absence epilepsy rats from Strasbourg (GAERS). Using quantitative RT-PCR and in situ hybridization, no difference was found in alpha1G mRNA expression between GAERS and control animals, while a decreased expression of alpha1E was seen in the cerebellum and the brain stem of the GAERS. This phenomenon was not observed in young animals when the epileptic phenotype is not expressed.


Subject(s)
Calcium Channels/genetics , Epilepsy, Absence/metabolism , Nerve Tissue Proteins/genetics , RNA, Messenger/metabolism , Animals , Brain Stem/metabolism , Calcium Channels, R-Type , Cation Transport Proteins , Cerebellum/metabolism , Epilepsy, Absence/genetics , In Situ Hybridization , Isomerism , Male , Rats , Rats, Inbred Strains/genetics , Rats, Wistar , Reference Values , Reverse Transcriptase Polymerase Chain Reaction
20.
J Periodontal Res ; 34(6): 323-8, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10633887

ABSTRACT

Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated "first transferred cells" (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy--phase contrast, scanning, or transmission electron--or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome-labelled cells could be detected in all these.


Subject(s)
Fibroblasts/transplantation , Gingiva/transplantation , Animals , Cell Culture Techniques/methods , Cell Separation/methods , Cells, Cultured , Fibroblasts/cytology , Fluorescent Dyes , Gingiva/cytology , Male , Microscopy, Fluorescence , Rats , Rats, Wistar , Syringes , Transplantation, Autologous
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