[The mode of identification of microscopic fungi of genus of Coccididoides spp. in vitro].

The article deals with analysis of morphologic characteristics of microscopic fungi of genus of Coccididoides spp. under cultivation on culture of mouse splenocytes culture. During two days, the strains of C. imitis and C. posadasii converse from filamentous to spherulic form. This process makes it possible to apply this test to identify agents of coccidioidomycosis.

[The morphologic characteristics of microscopic fungi of genus coccidioides].

The morphologic characteristics of microscopic fungi of genus Coccidioides under cultivation in nutrient mediums are studied. It is demonstrated that filamentous form of agents of coccidioidomycosis is characterized by significant polymorphism of macro- and micromorphologic signs on different stages of development ofagar culture. But C. immitis and C. posadasii have no species' differences. The dynamics of development of coccidioidomycosis strains in microcultures is analyzed as well as the intensity of sporification. The forms and sizes of arthroconidiae are also established.

[Molecular methods of detection and identification of pathogenic Burkholderia].

Molecular diagnostic kits for detection and identification of agents of melioidosis and glanders on environmental objects and in clinical material are described. It was demonstrated that PCR with use of specific primers on the basis of different genetic targets could be useful for determination of generic, inter- and intraspecies belonging of pathogenic Burkholderia as well as for epidemiologic inspection of territories where melioidosis is enzootic.

[Identification and differentiation of pathogenic Burkholderia]. / Identifikatsiia i differentsial'naia diagnostika patogennykh burkkholderii.

In this review modern methods for the identification and differential diagnostics of the causative agents of glanders and melioidosis, recently included into the genus Burkholderia, are presented. The known phenotypic signs and genetic markers permitting the identification of two pathogenic microorganisms on the definite taxonomic level are described.

[Effect of cultivation temperature on the extrachromosomal hereditary factors of the causative agent of melioidosis]. / Vliianie temperatury kul'tivirovaniia na vnekhromosomnye faktory nasledstvennosti vozbuditelia melioidoza.

The cultivation temperature of Burkholderia pseudomallei has been shown to determine both the direction of morphological dissociation and the prophage induction rate. Inheriting plasmid replicons was found to depend on the temperature conditions during the growth of these bacteria. No influence of B. pseudomallei plasmids pPM1 and pCM2 on the lysogenic state of the bacterial cell and the formation of different B. pseudomallei variants was noted.

[Spontaneous phage production in Pseudomonas pseudomallei and in a range of hosts of melioidosis phages among representatives in the genus Pseudomonas]. / Izuchenie spontannoi fagoproduktsii Pseudomonas pseudomallei i kruga khoziaev melioidoznykh fagov sredi predstavitelei roda Pseudomonas.

A capacity to spontaneous phage production has been studied in 57 melioidosis museum strains. The strains were comparatively analyzed by the levels of frequencies of spontaneous phage production. It is established that 40 of 57 studied strains of Pseudomonas pseudomallei are capable to spontaneously produce phages with frequency from 10(7) to 1. On the indicator lawn of P. mallei phages formed negative colonies of different morphology transparent, turbid and turbid with secondary growth in the centre. No distinct correlation between the frequency of spontaneous phage production and morphology of negative colonies was revealed. The range of hosts of melioidosis phages was determined among 88 representatives of Pseudomonas genus. All melioidosis strains were resistant to their own phages. Phages, identical by the spectrum of lithic effect, were not found even in the case of quantitative coincidence of sensitive strains. Maximum activity was observed in 8 lysates which were lysed 42 to 53 strains of 88 under study.