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Anal Chem
; 63(4): 384-90, 1991 Feb 15.
Article
in English
| MEDLINE
| ID: mdl-1823727
ABSTRACT
Surfactant-containing eluents are evaluated for possible application to direct serum injection using conventional reverse-phase columns. Serum albumin was quantitatively eluted at the column void volume by using surfactant concentrations below or above the critical micelle concentration, and organic solvents could be used in proportions as high as 40% (w/w). Surfactant choice, pH, and salt effects were also evaluated.