[Processes of bacterial L transformation and L form reversion in the body of argasid ticks]. / Protsessy L-transformatsii bakterii i reversii L-form v organizme argasovykh kleshchei.

The experimental infection of tampan ticks (Ornithodoros moubata) with the bacterial cultures of Listeria monocytogenes and Salmonella typhimurium, as well as with their L-forms, was carried out. These experiments demonstrated that both the L-transformation of bacteria and the reversion of their L-forms into the initial bacterial culture could occur in the body of the ticks.

[Blood-sucking arthropods as a factor in a natural focus of human diseases]. / Krovososushchie chlenistonogie kak faktor prirodnoi ochagovosti boleznei cheloveka.

Analysis of epidemiological and general biological aspects of modern concept of natural nidality of diseases of man has shown that obligato-transmissible diseases hold a prominent place in the phenomenon of natural nidality. The greater the role of the transmissible factor in the ecology of an infection, the better the main characters of natural nidality are displayed on its example. Population-ecological, immunological and genetic investigations of relations between bloodsucking arthropods and agents of natural-nidal infections are given much attention.

[Comparative study of the bactericidal action of the body of Ornithodoros papillipes ticks]. / Sravnitel'noe izuchenie bakteritsidnogo deistviia organizma kleshchei Ornithodoros papillipes.

Experiments in vivo have shown that the ticks of Ornithodoros papillipes inhibit both Gram-positive and Gram-negative bacteria. On Gram-positive microorganisms most sensitive to bactericidal effect of ticks have turned to be micrococci, staphylococci, streptococci and bacteria of diphtheria, of Gram-negative ones--Bacillus coli. Of Gram-positive microorganisms less sensitive were Listeria, of Gram-negative ones--salmonellae, agents of tularemia and pseudotuberculosis. At the infection with bacteria the organism of the tick produces a bactericidal substance which causes the dying off of the microbal population. The ability of the bactericidal substance to lyse the inhibited cells of Micrococcus lysodeiktickus as well as the coincidence of data on sensibility to the tick bactericidal substance obtained experimentally in vitro and in vivo have shown that lysozyme is an active antibacterial substance in the tick's organism.

[Argasid tick lysozyme action on HEp-2 cells]. / Izuchenie deìstviia lizotsima argasovykh kleshchei na kletki linii HEp-2.

The cytotoxic effect on Argasidae lysozyme was shown on a model of cell line HEp-2 in comparison to egg lysozyme. The lysozyme was obtained from homogenates of Ornithodoros papillipes of subfamily Ikodoidea. The lysozyme in concentrations of 300 and 500 gamma/ml had a cytotoxic effect, while in doses of 50 and 100 gamma/ml it has no such activity. The cells of line FL were not sensitive to the above concentrations of the lysozyme. In concentrations of 500 gamma/ml and higher the egg lysozyme had an analogous cytotoxic effect on the cells of line Hep-2. The comparative study of the cariogrammes of the cell monolayer treated with the Argasidae and egg lysozymes, as well as the study of the level of 3H-thimidine incorporation into the cell DNA showed the absence of the preparations effect of the mitotic activity of the cells and DNA synthesis by them.