ABSTRACT
Cervical carcinoma is the leading cause of cancer death in Quechua indians from Jujuy (northwestern Argentina). To determine the prevalence of HPV-16 variants, 106 HPV-16 positive cervical samples were studied, including 33 low-grade squamous intraepithelial lesions (LSIL), 28 high-grade squamous intraepithelial lesions (HSIL), 9 invasive cervical cancer (ICC), and 36 samples from women with normal colposcopy and cytology. HPV genome variability was examined in the L1 and E6 genes by PCR-hybridization. In a subset of 20 samples, a LCR fragment was also analyzed by PCR-sequencing. Most variants belonged to the European branch with subtle differences that depended on the viral gene fragment studied. Only about 10% of the specimens had non-European variants, including eight Asian-American, two Asian, and one North-American-1. E6 gene analysis revealed that 43% of the samples were identical to HPV-16 prototype, while 57% corresponded to variants. Interestingly, the majority (87%) of normal smears had HPV-16 prototype, whereas variants were detected mainly in SIL and ICC. LCR sequencing yielded 80% of variants, including 69% of European, 19% Asian-American, and 12% Asian. We identified a new variant, the Argentine Quechua-51 (AQ-51), similar to B-14 plus two additional changes: G7842-->A and A7837-->C; phylogenetic inference allocated it in the Asian-American branch. The high proportion of European variants may reflect Spanish colonial influence on these native Inca descendants. The predominance of HPV-16 variants in pathologic samples when compared to normal controls could have implications for the natural history of cervical lesions.
Subject(s)
Genetic Variation , Indians, South American , Papillomaviridae/classification , Papillomaviridae/genetics , Uterine Cervical Neoplasms/epidemiology , Argentina/epidemiology , Argentina/ethnology , Cervix Uteri/virology , Female , Humans , Molecular Sequence Data , Papillomavirus Infections/epidemiology , Papillomavirus Infections/virology , Polymerase Chain Reaction , Sequence Analysis, DNA , Tumor Virus Infections/epidemiology , Tumor Virus Infections/virology , Uterine Cervical Neoplasms/virology , Viral Proteins/chemistry , Viral Proteins/genetics , Uterine Cervical Dysplasia/epidemiology , Uterine Cervical Dysplasia/virologyABSTRACT
Human Papillomaviruses (HPVs) are etiologically associated to cervical carcinoma. In order to evaluate HPV infection and its relationship with the high frequency of this neoplasia in Quechua women from Jujuy (Argentina), 271 cervical samples from preneoplastic and neoplastic lesions (biopsies) and normal controls (cytologies) were studied. Detection and typing were performed using PCR-RFLP or PCR-hybridization and the HPV-16 variability in L1 and E6 genes (by PCR-hybridization) was analysed. HPV was detected in 52% of controls, 91% of low-grade lesions, 97% of high-grade lesions and 100% of invasive carcinomas, corresponding 55% to HPV-16. HPV-16 European variants were predominant, most of them being non-prototypic strains. The high frequency of high risk infection types and the raised proportion of HPV-16 non-prototypic variants related to a greater oncogenic potential could explain, in part, the high cervical cancer frequency of this native population. These data may contribute to disease control and vaccinal formulation.
Subject(s)
Genetic Variation , Papillomaviridae/genetics , Papillomavirus Infections/virology , Tumor Virus Infections/virology , Uterine Cervical Neoplasms/virology , Adolescent , Adult , Argentina/epidemiology , Argentina/ethnology , Female , Humans , Incidence , Middle Aged , Papillomaviridae/isolation & purification , Papillomavirus Infections/epidemiology , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Prevalence , Tumor Virus Infections/epidemiology , Uterine Cervical Neoplasms/epidemiologyABSTRACT
Human Papillomaviruses (HPVs) are etiologically associated to cervical carcinoma. In order to evaluate HPV infection and its relationship with the high frequency of this neoplasia in Quechua women from Jujuy (Argentina), 271 cervical samples from preneoplastic and neoplastic lesions (biopsies) and normal controls (cytologies) were studied. Detection and typing were performed using PCR-RFLP or PCR-hybridization and the HPV-16 variability in L1 and E6 genes (by PCR-hybridization) was analysed. HPV was detected in 52
of controls, 91
of low-grade lesions, 97
of high-grade lesions and 100
of invasive carcinomas, corresponding 55
to HPV-16. HPV-16 European variants were predominant, most of them being non-prototypic strains. The high frequency of high risk infection types and the raised proportion of HPV-16 non-prototypic variants related to a greater oncogenic potential could explain, in part, the high cervical cancer frequency of this native population. These data may contribute to disease control and vaccinal formulation.
ABSTRACT
Human Papillomaviruses (HPVs) are etiologically associated to cervical carcinoma. In order to evaluate HPV infection and its relationship with the high frequency of this neoplasia in Quechua women from Jujuy (Argentina), 271 cervical samples from preneoplastic and neoplastic lesions (biopsies) and normal controls (cytologies) were studied. Detection and typing were performed using PCR-RFLP or PCR-hybridization and the HPV-16 variability in L1 and E6 genes (by PCR-hybridization) was analysed. HPV was detected in 52 of controls, 91 of low-grade lesions, 97 of high-grade lesions and 100 of invasive carcinomas, corresponding 55 to HPV-16. HPV-16 European variants were predominant, most of them being non-prototypic strains. The high frequency of high risk infection types and the raised proportion of HPV-16 non-prototypic variants related to a greater oncogenic potential could explain, in part, the high cervical cancer frequency of this native population. These data may contribute to disease control and vaccinal formulation (AU)
Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , RESEARCH SUPPORT, NON-U.S. GOVT , Human Papillomavirus Viruses/genetics , Papillomavirus Infections/epidemiology , Tumor Virus Infections/epidemiology , Uterine Cervical Neoplasms/epidemiology , Genetic Variation , Human Papillomavirus Viruses/isolation & purification , Papillomavirus Infections/genetics , Tumor Virus Infections/genetics , Uterine Cervical Neoplasms/genetics , Uterine Cervical Neoplasms/virology , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Base Sequence , Nucleic Acid Hybridization , Incidence , Prevalence , Argentina/epidemiologyABSTRACT
Human Papillomaviruses (HPVs) are etiologically associated to cervical carcinoma. In order to evaluate HPV infection and its relationship with the high frequency of this neoplasia in Quechua women from Jujuy (Argentina), 271 cervical samples from preneoplastic and neoplastic lesions (biopsies) and normal controls (cytologies) were studied. Detection and typing were performed using PCR-RFLP or PCR-hybridization and the HPV-16 variability in L1 and E6 genes (by PCR-hybridization) was analysed. HPV was detected in 52 of controls, 91 of low-grade lesions, 97 of high-grade lesions and 100 of invasive carcinomas, corresponding 55 to HPV-16. HPV-16 European variants were predominant, most of them being non-prototypic strains. The high frequency of high risk infection types and the raised proportion of HPV-16 non-prototypic variants related to a greater oncogenic potential could explain, in part, the high cervical cancer frequency of this native population. These data may contribute to disease control and vaccinal formulation
