Retroviral particles produced from a stable human-derived packaging cell line transduce target cells with very high efficiencies.

The goal of this work was to determine whether a stable 293 amphotropic packaging line, which we have designated 293-SPA, is useful for the production of high-titer stable virus by comparison to the murine psiCRIP line. Here, we report our unexpected findings that particles derived from the 293-SPA line transduce target cells (both NIH-3T3 cells and primary melanoma cells) with greatly enhanced efficiencies (at least 10-fold) compared to particles derived from the psiCRIP packaging line. We show that the presence of a transferable inhibitor in the psiCRIP line at least partially accounts for this dramatic difference in transduction efficiency. This work has important implications for improving the efficiency of retrovirus-mediated gene transfer in general as well as in the design of new packaging cell lines.

Color Doppler sonography of benign and malignant ovarian masses.

The use of transvaginal and transabdominal color Doppler sonography for the assessment of ovarian tumor vascularity was investigated in 62 cases of surgically excised and histologically examined ovarian masses. The modality was used to differentiate tumor neovascularity from normal arterioles (which contain smooth muscle in their media) on the basis of differences in the pulsatility observed in Doppler waveforms. Of the 25 malignant tumors in the series, 20 had low-impedance flow (pulsatility index of less than 1.0), and none had the diastolic notch seen in vessels in normal tissue. Three benign lesions, including two dermoid cysts and one tubo-ovarian abscess, also had low-impedance flow. The negative predictive value of color Doppler sonography was 98%, whereas the positive predictive value was 83%. Color Doppler sonography seems to be accurate for excluding malignancy, but some misdiagnosis may occur in cases of inflammatory and metabolically active benign masses.

Is pain sensitivity associated with occupational fears in police recruits?

Gross (Behaviour Research and Therapy, 30, 7-13, 1992a) showed that the fear of pain (pain sensitivity) could be distinguished from dental fears and health concerns. Furthermore, the Pain Sensitivity Index (PSI) correlated significantly with pain expectations, and pain expectations correlated significantly with dental avoidance behaviour. However, Gross noted that the psychometric properties of the PSI had not been fully determined. The present study examined the factor structure of an expanded version of the PSI with a sample of 212 police recruits. A single factor accounted for 52.1% of the variance and the reliability coefficient (alpha = 0.89) indicates that the PSI possesses a high degree of internal reliability. Subsequently, it was hypothesized that pain sensitivity correlates positively with fears relating to police tasks which are characterized by pain expectancies, such as the fear of being assaulted during operational police work. In support of the hypothesis, pain sensitivity correlated highest with situations involving pain. The pain sensitivity construct may help to explain individual differences in fear reactions to situations where pain could be experienced, such as operational police duties or dental attendances.

Is pain sensitivity associated with dental avoidance?

The present investigation examined whether pain sensitivity is associated with dental avoidance. Whereas the aim of the first study was to determine whether pain sensitivity can be distinguished from dental fears and health concerns, the aim of the second study was to examine the structure of cognitive aspects of pain sensitivity, and the aim of the third study was to examine the relationships of pain sensitivity to anxiety sensitivity, fear, pain, and avoidance. In the first study (n = 222), a factor analysis performed with items from the Dental Phobia and Pain Sensitivity Inventory distinguished a pain sensitivity factor from factors relating to dental phobia and health concerns. The factor structure of the Pain Sensitivity Index was examined in the second study (n = 181), and similarly, a pain sensitivity factor was interpreted in the data. The third study (n = 65) found that although pain sensitivity correlated significantly with anxiety sensitivity, these constructs are not identical to each other. For instance, pain sensitivity but not anxiety sensitivity correlated significantly with blood-injury phobia. Pain sensitivity should be taken into account in future studies of dental phobia as pain sensitivity was found to be predictive of pain intensity, and pain intensity was found to be predictive of dental avoidance.

Neutrophilic eccrine hidradenitis: a case report and review of the literature.

A case of neutrophilic eccrine hidradenitis is presented, with a review of the literature. Although the histologic appearance of this disorder is distinct and infrequently reported, the clinical presentation is common. Neutrophilic eccrine hidradenitis may be an under-reported benign drug reaction in patients with white blood cell disorders.

Components of generalized anxiety: the role of intrusive thoughts vs worry.

This study explored possible mechanisms involved with the maintenance of generalized anxiety. While several general anxiety-related variables were investigated, the relationship between worry and intrusive thoughts was of primary interest. We postulated that anxious persons continue to worry in order to avoid recollections of distressing life events. Accordingly, we hypothesized that worry can be distinguished from intrusive thoughts and that the experience of worry would be preferred to intrusive thoughts. Results of a factor analysis indicated that worry and intrusive thought items loaded on separate factors, which supports the hypothesis that worry can be distinguished from intrusive thoughts. The study also examined whether different patterns of worry and intrusive thoughts distinguish between high-anxious, panic, and low-anxious persons and how anxiety sensitivity and self-consciousness are related to generalized anxiety and panic. Finally, we discussed the implications of our results for understanding the psychopathology base of Generalized Anxiety Disorder.

Phobic disorders and anxiety states: how do they differ?

A review of the clinical literature to date has shown that the nature of the relationship between phobic disorders and anxiety states is still unclear. As a wide range of symptoms are shared by patients with all DSM-III anxiety disorder diagnoses, at this stage there is still a need to investigate the latent dimensions which distinguish the anxiety disorder subtypes. In the present study 176 patients with the DSM-III diagnoses of agoraphobia with panic attacks, social phobia, panic disorder and generalized anxiety disorder completed the Fear Survey Schedule, Fear Questionnaire, Hostility and Direction of Hostility Questionnaire, Maudsley Personality Inventory, and the Hamilton Anxiety and Depression Scales. Group membership was significantly predicted by a discriminant analysis which yielded a Fear Questionnaire agoraphobia function and a social phobia function. The results from discriminant analysis suggests that agoraphobia and anxiety states may be closely related. Classification errors were also determined, providing further evidence with which to refute the claim that agoraphobia has "all or none" characteristics.

Preparation book for hospitalized pediatric patients: content and design.

In the past 25 years, research has indicated the need for increased education and emotional support for pediatric patients in order to lessen their stress during hospitalization. A preparatory coloring book can help meet this need by providing factual information and by allowing emotional expression. A review of the literature identifies the major fears that should be addressed and gives guidelines for effectively producing such a book.

Oral vitamin A in acne vulgaris. Preliminary report.

Oral vitamin A (retinol) is generally not considered useful in the treatment of acne vulgaris. We conducted a study which showed that retinol was indeed ineffective at the usual doses of 50,000 to 100,000 IU daily. Retinol was highly efficacious in doses of 300,000 units for women and 400,000 to 500,000 units for men, toxicity was slight and limited mainly to skin (xerosis) and mucous membranes (cheilitis). The danger of hypervitaminosis A in this dosage range has been exaggerated. Retinol is a valuable drug for treating stubborn, severely inflammatory acne vulgaris. It is administered until the disease is brought under control, usually within three to four months. Then the dosage is progressively reduced relying on conventional drugs to keep the disease in abeyance.

Histone gene expression: progeny of isolated early blastomeres in culture make the same change as in the embryo.

Stage-specific changes in histone synthesis during sea urchin development reflect the expression of different sets of genes. The three kinds of blastomeres formed at the 16-cell stage are the earliest "determined" cells and fall into three distinct size classes. At this stage that cells synthesize only "early" histones. Such blastomeres can survive and divide in culture after being separated from the embryo, whether or not they are permitted to aggregate. With or without reaggregation, cultured progeny cells of each type of isolated blastomere perform the same changeover of histone synthesis as takes place in the intact embryo, that is, they begin spontaneously to synthesize a new set, the "late" histone variants. Normal contact relations among cells of the embryo are, therefore, not required for this programmed change in gene expression.

Lymphomatoid granulomatosis.

The exact nature of lymphomatoid granulomatosis remains unclear. Whether it represents a viral infection, an autoimmune disorder (possibly induced by a virus), or primary malignant lymphoma, is still to be determined. The histopathology and clinical course allows classification of lymphomatoid granulomatosis as a distinct entity. Unfortunately, there is no consistently effective therapy for this condition and the usual course is rapidly downhill, though temporary remissions may be obtained. Since presentation of these patients with cutaneous lesions is not unusual, dermatologists must be alert to the possibility of an underlying systemic vasculitis in patients with atypical cutaneous ulceration, especially when no obvious cause is apparent.