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Article in English | MEDLINE | ID: mdl-14565445

ABSTRACT

A four-color set of negatively charged, single dye as well as energy transfer dye labeled-ddNTPs were synthesized and evaluated in combination with a novel polymerase in a "direct-load" DNA sequencing, obviating the laborious and time consuming post-reaction work-up.


Subject(s)
Coloring Agents , DNA/chemistry , Deoxyribonucleotides/chemical synthesis , Dideoxynucleosides/chemical synthesis , Base Sequence , Energy Transfer , Indicators and Reagents
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