ABSTRACT
Myxococcus xanthus is a soil bacterium with a complex life cycle involving distinct cell fates, including production of environmentally resistant spores to withstand periods of nutrient limitation. Spores are surrounded by an apparently self-assembling cuticula containing at least Proteins S and C; the gene encoding Protein C is unknown. During analyses of cell heterogeneity in M. xanthus, we observed that Protein C accumulated exclusively in cells found in aggregates. Using mass spectrometry analysis of Protein C either isolated from spore cuticula or immunoprecipitated from aggregated cells, we demonstrate that Protein C is actually a proteolytic fragment of the previously identified but functionally elusive zinc metalloprotease, FibA. Subpopulation specific FibA accumulation is not due to transcriptional regulation suggesting post-transcriptional regulation mechanisms mediate its heterogeneous accumulation patterns.
Subject(s)
Antigens, Bacterial/metabolism , Extracellular Space/enzymology , Membrane Proteins/metabolism , Metalloproteases/metabolism , Myxococcus xanthus/cytology , Myxococcus xanthus/enzymology , Peptide Fragments/metabolism , Protein C/metabolism , Antigens, Bacterial/chemistry , Gene Expression Regulation, Bacterial , Mass Spectrometry , Membrane Proteins/chemistry , Metalloproteases/chemistry , Molecular Weight , Myxococcus xanthus/genetics , Myxococcus xanthus/physiology , Promoter Regions, Genetic/genetics , Protein C/chemistry , Spores, Bacterial/cytology , Spores, Bacterial/enzymology , Spores, Bacterial/genetics , Subcellular Fractions/metabolism , Transcription, GeneticABSTRACT
A new series of macrocyclic complexes of type [M(TML)X]X(2), where M = Cr(III), Fe(III), TML is tetradentate macrocyclic ligand, and X = Cl(-), NO(3)(-), CH(3)COO(-), have been synthesized by condensation of isatin and ethylenediamine in the presence of metal salt. The complexes were synthesized by both conventional and microwave methods. The complexes have been characterized with the help of elemental analysis, conductance measurement, magnetic measurement, and infrared, far infrared, and electronic spectral studies. Molar conductance values indicate them to be 1:2 electrolytes. Electronic spectra along with magnetic moments suggest five-coordinate square pyramidal geometry for these complexes. The complexes were also tested for their in vitro antibacterial activity. Some of the complexes showed satisfactory antibacterial activitiy.
Subject(s)
Anti-Bacterial Agents/chemistry , Coordination Complexes/pharmacology , Macrocyclic Compounds/chemical synthesis , Bacteria/drug effects , Coordination Complexes/chemical synthesis , Coordination Complexes/chemistry , Ethylenediamines/chemistry , Isatin/chemistry , Macrocyclic Compounds/chemistry , Macrocyclic Compounds/pharmacology , Metals, Heavy/chemistryABSTRACT
A novel series of the complexes of the type:[M(TML)X]X2; where TML is a tetradentate macrocyclic ligand; M=Cr(III), Fe(III); X=Cl-, NO3-, OAc- has been synthesized by condensation of indole-2,3-dione (isatin) and o-phenylenediamine in the methanolic medium. The complexes have been characterized with the help of various physicochemical techniques like elemental analyses, conductance measurements, magnetic measurements, infrared and electronic spectral studies. Molar conductance values indicate they are 1:2 electrolytes. Electronic spectra along with magnetic moments suggest the five coordinate square-pyramidal geometry for these complexes. The complexes were also tested for their in vitro antibacterial activities. Some of the complexes showed remarkable antibacterial activities against some of the selected bacterial strains.
