ABSTRACT
The characteristics of viral-bacterial lesion in the appendix of children with appendicitis are given by means of histologic, histochemical, immunomorphological and morphometric methods. Antigens of influenza viruses A, B, C, entero-, adeno- and paramyxoviruses were observed among which influenza C was found significantly more frequently (64.1%). Viral infection in the majority of cases (79.5%) was found in the association with opportunistic flora, more frequently with E. coli, and less R. aeruginosa and Klebsiella. Analysis of the immunomorphological shifts in the appendix indicated a peculiar dynamics of the antibody production in acute appendicitis: the enhancement of the IgG, IgD and IgE production and the decrease of the production of IgA and IgM. The viral-bacterial effect, the development of the immune responses with IgE hyperproduction and participation in this process of labrocytes and endocrine cells suggest the integration of the two pathogenetic mechanisms-infectious and allergic.
Subject(s)
Appendicitis/pathology , Appendix/pathology , Bacterial Infections/pathology , Virus Diseases/pathology , Acute Disease , Adolescent , Antigens, Viral/analysis , Appendicitis/immunology , Appendix/immunology , Appendix/microbiology , Bacteria/isolation & purification , Bacterial Infections/immunology , Bacterial Infections/microbiology , Cellulitis/immunology , Cellulitis/microbiology , Cellulitis/pathology , Child , Child, Preschool , Humans , Immunoglobulins/analysis , Infant , Virus Diseases/immunology , Virus Diseases/microbiologyABSTRACT
Signet-cell carcinoma of Brunner's glands and two chronic ulcers of the duodenum were revealed in the course of the histologic examination of the autopsy material from a 76-year-old patient. One of the ulcers penetrated into the pancreas. This case is interesting for rare occurrence of Brunner's glands carcinoma and its combination with chronic duodenum ulcers.
Subject(s)
Adenocarcinoma, Mucinous/pathology , Brunner Glands/pathology , Duodenal Neoplasms/pathology , Duodenal Ulcer/pathology , Adenocarcinoma, Mucinous/complications , Aged , Chronic Disease , Duodenal Neoplasms/complications , Duodenal Ulcer/complications , Humans , MaleSubject(s)
Intestinal Mucosa/cytology , Animals , Animals, Suckling , Cell Differentiation , Cell Separation , Cells, Cultured , Embryo, Mammalian , Epithelial Cells , Fetus , Humans , Intercellular Junctions , Intestine, Large/cytology , Intestine, Small/cytology , Microscopy, Electron , Microscopy, Electron, Scanning , Microvilli , Organ Culture Techniques , Organoids/ultrastructure , Rats , Time FactorsABSTRACT
Ultrastructure of the primary culture of epitheliocytes in the small and large intestine of 20-22-week-old human fetuses has been investigated, normal and 1, 3, 6, 12 and 24 h after administration of cholera toxin (choleragen) into the cell culture. The culture studied is mainly presented by absorbtive epitheliocytes, goblet, endocrinic and Paneth's cells, that preserve to a certain degree their differented structure. The most pronounced ultrastructural changes of the intestinal epitheliocytes in the primary cell culture, resembling those, that are previously noted in the epitheliocytes of the small and large intestine under influence of cholera toxin in various experimental animals in vivo, are revealed 3-6 h after administration of cholera toxin into the primary culture of the small intestine epitheliocytes and 6-12 h--into the large intestine epitheliocytes. The intestinal epitheliocytes of the human fetuses in the cell culture are sensitive to the action of cholera toxin and present a suitable model for studying the action mechanism of the toxin on the intestinal epithelium.
