[Surgical intraduodenal drainage of bile duct: an introductory report]. / Operacyjny dodwunastniczy drenaz drogi zólciowej--doniesienie wstepne.

UNLABELLED: Reconstructing operations, performed on extrahepatic bile ducts, usually need protection of anastomosis by means of drainage. Classic Kehr's drainage is loaded with the risk of complications. Since 1993 the authors have been applying the alter way of drainage: an intraduodenal drainage by endoprothesis Y (DEY). Endoscopical removal of the drain was performed in 3-4 months after the operation. This way of drainage was used between III 1993--V 1997 in 23 patients. We did not observed any complications or pancreas reactions. Clinical control of patients was made after operation with ECW. In 4 cases the results of treatment were unsuccessful. In other 16 patients results of performed operations were favourable. CONCLUSION: Proposed way of protection of bile duct's reconstruction's site using DEY decreases the number of complications connected with classic Kehr's drainage.

[Experimental evaluation of vascularization by various methods of forming a synthetic stomal pseudosphincter]. / Doswiadczalna ocena unaczynienia róznie wytworzonego sztucznego zwieracza przetoki kalowej.

Authors presents the own method of formation of the stomal pseudosphincter using a pedunculated intestinal flap devoid of mucosa. The experimental and clinical evaluation of blood supply of the sphincter was carried out by means of the doppler and fluorescence method. The study revealed no impairment of the blood supply of the sphincter and it's connect function.

[Evaluation of stomach emptying under extreme obstruction of gastrointestinal transit treated with gastro-intestinal or duodenal-intestinal anastomosis]. / Ocena oprózniania zoladkowego w warunkach wysokiej niedroznosci przewodu pokarmowego leczonej zespoleniem zoladkowo-jelitowym lub dwunastniczo-jelitowym.

The purpose of the present study was to compare gastric emptying in two groups of dogs in which a gastrojejunal or duodenojejunal Roux-en-Y anastomosis was performed over the site of an experimental obstruction in the distal duodenum. The experiment was carried out on 10 mongrel dogs. Gastric emptying was assessed twice in each dog before the experiment (control examination); the solid phase of the test meal was labelled with 99mTc. Following a control examination, the dogs were divided into two groups of 5 animals each, and subjected to the above mentioned surgical procedures. Postoperative gastric emptying was carried out 3 weeks after the operation, and then at 3 and 6 months following the procedure. The following parameters describing quantitatively gastric emptying were determined: mean transit time MTT0-60. MTT0-120 and total mean transit time MTT0-180. The comparison of these parameters revealed statistically significant differences confirming delay of gastric emptying in dogs with a gastrojejunal anastomosis.

Activation of duck prothrombin by factor Xa and thrombin.

Prothrombin isolated from duck sodium citrate plasma was activated in a system containing duck factor Xa and calcium ions. Polyacrylamide gel electrophoresis showed that intermediates and the final product, thrombin, of Mr in the range 21 500-52 000 were present in the incubation mixture Serine and isoleucine were found to be the N-terminal amino acids of the intermediate form 1 and thrombin, respectively.

Characterization of the Forssman-active oligosaccharides from dog gastric mucus glycoprotein isolated with the use of a monoclonal antibody.

The structure of carbohydrate chains of dog gastric mucus glycoprotein bearing Forssman antigenic determinant was investigated. The reduced oligosaccharides obtained by alkaline borohydride reductive cleavage of the glycoprotein were chromatographed on a immunoaffinity column containing monoclonal anti-Forssman antibody coupled to cyanogen bromide-activated Sepharose 4B. The bound oligosaccharides, eluted from the affinity column with 6 M urea, separated on thin layer chromatography into two major components. These two compounds, representing 92% of the absorbed oligosaccharides, were purified to homogeneity, and their structures and antigenic properties were determined. Based on the results of immunological assays, analysis of sugar composition, sequential degradation with specific glycosidases, and methylation analyses of the intact and enzyme-degraded compounds, we propose the following structures for these oligosaccharides. (formula, see text).

Characterization of the sulfated monosialosyltriglycosylceramide from bovine gastric mucosa.

A sulfated monosialosyltriglycosylceramide was isolated from the lipid extract of bovine gastric mucosa in a yield of 2.52 microM/kg of wet tissue. The isolation procedure involved extraction of lipids with 0.4 M sodium acetate in methanol/chloroform/water, alkaline methanolysis, column fractionation on DEAE-Sephadex and silicic acid, and thin-layer chromatography. Based on the results of partial acid hydrolysis, sequential degradation with specific glycosidases, periodate oxidation, and methylation analysis of the native and partially degraded compound, the structure of this glycolipid is proposed to be: GalNAc beta 1 leads to 4(SO3H leads to 8Sia alpha 2 leads to 3)Gal beta 1 leads to 4Glc1 leads to 1Cer. The sialic acid of this glycolipid consisted of N-acetylneuraminic acid and N-glycoloylneuraminic acid, with the former being predominant component (71%) of the mixture.

Glyceroglucolipids of the mucous barrier of dog stomach.

Distribution of glyceroglucolipids in the mucous barrier of dog stomach fundus, body and antrum was investigated. Surface mucus lining and preformed intracellular mucus were obtained by instillation of the ligated stomach compartments with 2 M NaCl. Lipids were extracted from the dialyzed and lyophilized samples, and the glycolipids were separated into neutral and acidic fractions. The glyceroglucolipids contained in each fraction were purified into individual components by thin-layer chromatography and were quantified. The content of glyceroglucolipids (mumol glucose/g protein) in the antral portion of the stomach was 2.1 times greater than that of the fundus and 3.4 times greater than that of the body. All three areas of the stomach contained neutral and sulfated glyceroglucolipids. However, the level of sulfated glycerolglucolipids (mumol glucose/g protein) was three times higher in the antrum as compared to the fundus and four times higher as compared to the body. The neutral and sulfated glyceroglucolipids were present in a molar ratio of 1.0 : 0.7 in the fundus, 1.0 : 1.1 in the body and 1.0 : 1.5 in the antrum.