Metallothione in levels in cell fractions from breast cancer tissues.

Metallothioneins (MTs) protect the cell against reactive forms of oxygen, ionizing radiation, pharmacological agents and mutagens. Metallothioneins are also responsible for neoplastic cell resistance to cytostatic drugs. The aim of this study was to determine the MT level in cell fractions as well as to determine whether there is any change in the concentration of these proteins in a neoplastic cell, and in which cell fractions this change takes place. The neoplastic tissue examined was histopathologically ductal carcinoma invasive, and the control tissue was mastopathic tissue with proliferated connective and glandular tissue. The level of MTs was determined using cadmium isotope (109Cd). It was determined that there was an increase in MT level in the neoplastic tissue, the highest level being found in the mitochondrial fraction obtained from the control and mastopathic tissues. The greatest changes in MT concentrations in breast carcinoma were observed in the nuclear and cytosol fractions. In the nuclear fraction in the breast carcinoma tissue, the MT level was almost three times that of the control group.

[Activity of isocitrate dehydrogenase in breast cancer]. / Aktywnosc dehydrogenazy izocytrynianowej w raku sutka.

The activity of isocitrate dehydrogenase (ICDH) was studied in the tissue of breast cancer and in the blood serum of the women suffering from this neoplasm. The comparative material was the physiological tissue of mammae and blood serum of the healthy women. The activity of ICDH in neoplasm tissue was more higher than in physiological tissue. In the blood serum of ill patients activity of this enzyme was higher than in control serums too.

[Angiofollicular lymph node hyperplasia (Castelman's disease) in an adult woman]. / Naczyniowo-grudkowy rozrost wezlów chlonnych (choroba Castlemana) u doroslej kobiety.

Angiofollicular lymph node hyperplasia Castleman's disease) is a rare disorder of unclear etiology and wide spectrum of systemic symptoms. This report describes a case of this disorder of hyalinovascular type in 45-years old woman. The lesions were localized in axillary lymph nodes. The disease was curable by surgical excision.

[Histiocytosis X]. / Histiocytoza X.

Histiocytosis X comprises a spectrum of diseases of unknown etiology in which localized or systemic histiocytic proliferations occur, often associated with eosinophilic infiltration of the involved tissues. The three clinical syndromes are: eosinophilic granuloma, Hand-Schuller-Christian disease and Abta-Letteres-Siwesche's disease. Although diagnosis is based on the histochemical findings, the "definite" diagnosis requires the presence of Langerhans' granules in histiocytes. Localized histiocytosis X is treated by surgical excision and irradiation. Corticosteroid treatment is effective in symptomatic control in most patients and a variety of cytotoxic drugs have been used either alone or in combination with corticosteroids.