ABSTRACT
OBJECTIVE: The seeds of Pulsatilla cernua were used as tested materials for screening and establishing the main factors with different levels to control fast development of seed embryonic and seedlings of Pulsatilla cernua. METHODS: The main factors with different levels for development of seed embryonic and seedlings of Pulsatilla cernua were investigated through repeated experiments with multifactorial and cross. RESULTS: The method for development of seedlings and seeds germination of Pulsatilla cernua were to soak the seeds in the mixed solutions with 2.40 mg/L KT, 2.80 mg/L GA3 and 0.30 -0.70 mg/L 2,4-D for 24 h. The seeds and sand (1:2) were mixed, treated with temperature change in 63 - 70 d. The extent of temperature change and time were (23 ± 2) degrees C and 14 h in day, while (10 ± 2) degrees C and 10 h in night. The incidence rate of the embryo with cotyledons was 95.1%, and the germination rate of seed was 92.3%. CONCLUSION: The plant regeneration control technology for development of seed embryonic and seedling of Pulsatilla cernua have been solved, which is suitable for industrial seedlings of Pulsatilla cernua.