[The therapeutic effect of HSV1-hGM-CSF combined with doxorubicin on the mouse breast cancer model].

Objective: To evaluate the oncolytic effect of herpes simplex virus type 1 which carried recombined human granulocyte-macrophage colony-stimulating factor (HSV1-hGM-CSF) on the mouse breast cancer cell line 4T1 and compare the anticancer effects of HSV1-hGM-CSF, doxorubicin alone or combination on the breast cancer in mice. Methods: We investigated the cytotoxic effect on 4T1 cells in vitro, the cell growth, cell apoptosis and cell cycle of 4T1 cells treated with oncolytic HSV1-hGM-CSF at different MOIs (0, 0.5, 1 and 2) and doxorubicin at different concentrations (0, 2, 4 and 8 µg/ml). The effects of oncolytic HSV1-hGM-CSF and doxorubicin on the tumor growth, survival time and their side effects on the mouse breast cancer model were observed. Results: Both oncolytic HSV1-hGM-CSF and doxorubicin significantly inhibited the proliferation of 4T1 cells in vitro. Doxorubicin induced the G(2)/M phase arrest of 4T1 cells, while the cytotoxicity of oncolytic HSV1-hGM-CSF was no cell cycle-dependent.At day 16 after treatment with doxorubicin and HSV1-hGM-CSF, the tumor volume of 4T1 tumor bearing mice were (144.40±27.68)mm(3,) (216.80±57.18)mm(3,) (246.10±21.90)mm(3,) (327.50±44.24)mm(3,) (213.30±32.31)mm(3) and (495.80±75.87)mm(3) in the groups of doxorubicin combined with high dose HSV1-hGM-CSF, doxorubicin combined with low dose HSV1-hGM-CSF, doxorubicin alone, high dose HSV1-hGM-CSF alone, low dose HSV1-hGM-CSF alone and control, respectively.Compared with the control group, both doxorubicin and HSV1-hGM-CSF treatment exhibited significant reduction of primary tumor volume in vivo (P<0.001). The median survival times were 48, 50, 40, 42, 43 and 37 days in the six groups mentioned above, respectively. The median survival period of doxorubicin alone, high dose HSV1-hGM-CSF alone and low dose HSV1-hGM-CSF alone were significantly longer than that of control (P<0.05). Conclusion: Synergistic effect of sequential treatment with doxorubicin and oncolytic HSV1-hGM-CSF is observed in 4T1 mouse breast cancer.

BDNF improves the effects of neural stem cells on the rat model of Alzheimer's disease with unilateral lesion of fimbria-fornix.

In this study, neural stem cells (NSCs) were obtained from the hippocampus using the serum-free culturing. NSCs labeled with 5'-bromo-2'-deoxyuridine (BrdU) were transplanted into transected rat basal forebrain followed by the injection of brain-derived neurotrophic factor (BDNF) into the lateral ventricle. Nestin staining and double-labeling immunohistochemistry were used to detect cell survival and neuronal differentiation of the BrdU labeled cells in the basal forebrain and it was observed that labeled NSCs differentiated into neurons and astrocytes in the basal forebrain. Immunohistochemical detection of p75(NGFR) indicated that the number of cholinergic neurons of the combination groups treated by NSCs, BDNF, and NSCs groups had more significant improvement than that of the injured groups in medial septum (MS) and vertical diagonal branch (VDB). Learning and memory abilities were also measured by Y-maze test and the results support that BDNF can enhance the treatment effects of NSCs transplanted into brain lesion model.

[Effects of running armored car on dynamic posture equilibrium].

Objective. To study the effects of training in running armored car on dynamic posture equilibrium. Method. Dynamic posture was tested pre- and post running armored car in 56 healthy men. Result. Compared to pre-training, certain equilibrium scores of sensory organization test condition 1 (P<0.05), 2 (P< 0.001), and 3 (P< 0.001) decreased significantly, while those of condition 4 (P<0.001) and 6 (P< 0.001) increased significantly. The static proprioception score (SOT2/SOT1 x 100) (P< 0.01) decreased significantly, while visual sensory score (SOT4/SOT1 x 100, P <0.01) and dynamic proprioception (SOT (4+5+6)/SOT(1+2+3) x 100, P<0.001) increased significantly post-training. The motor strategy scores of sensory organization test condition 2 (P<0.01) and 3 (P<0.001) significantly decreased. Conclusion. The results demonstrated that training in a running armored car influenced postural equilibrium and sensory organization.

[Application of degree of complexity in heart rate variability analysis during orthostatic (correction of orthosatic) standing].

Objective. To introduce the degree of complexity in characterizing heart rate variability (HRV), and to discuss the changes in complexity of the cardiovascular system during orthostatic standing posture. Method. ECG of 8 subjects were recorded during supine and orthostatic standing postures. Degree of complexity was used to analyze the HRV. Result. Compared to supine before orthostatic standing posture, R-R intervals and its standard deviation at 0-5 min, 5-10 min, 10-15 min and 15-20 min during orthostatic standing posture were decreased significantly; the degree of complexity of HRV at 0-5 min and 15-20 min during orthostatic standing posture were decreased significantly; the approximate entropy of 0-5 min and 15-20 min were decreased significantly. Conclusion. The results showed that HRV and complexity of cardiovascular system was decreased during orthostatic standing. It was feasible that the degree of complexity can be used to analyze HRV.

[c-fos expression in rat's vestibular nucleus under eccentric rotational stimulation].

OBJECTIVE: To investigate the c-fos expression in rat's vestibular nucleus under eccentric rotational stimulation and the effects of anti-motion sickness drug (PAPM) on this expression. METHOD: 19 SD rats were divided into three groups: A, B and C. A received as control. B was stimulated by eccentric rotation for 60 minutes. C received injection of PAPM through cavun abdominis 45 min before eccentric rotation. Immunohistochemical method and computerized image analysis were used to map locations of c-fos protein appeared in four vestibular nucleus and to count the masculine cells. RESULT: c-fos protein appeared in four vestibular nucleus areas after stimulation, and PAPM had no influence on this expression. CONCLUSION: It suggests that c-fos expression in vestibular nucleus is one of the important way in which vestibular nervous system reacts to outside stimulation and this expression has no direct relationship with the generation and development of motion sickness.

[Approximate entropy and its application in heart rate variability analysis].

Objective. To introduce approximate entropy in the analysis of heart rate variability, and to discuss the changes of the complexity of cardiovascular system during orthostatic standing posture. Method. ECG of 8 subjects were recorded during supine and orthostatic standing postures. Approximate entropy was used to analyze HRV. Result. Compared to supine before orthostatic standing posture, R-R intervals at 0-5 min, 5-10 min, 10-15 min and 15-20 min during orthostatic standing posture were decreased significantly, while approximate entropy at 0-5 min and 15-20 min decreased significantly. It showed that the complexity of cardiovascular system was decreased and the modulation of cardiovascular system changed. Conclusion. It is feasible that approximate entropy be used for heart rate variability analysis.

[Effects of motion sickness evoked by parallel swing stimulation on posture equilibrium].

Objective. To study the effects of motion sickness on posture equilibrium. Method. Dynamic posture was tested pre- and following motion sickness evoked by parallel swing. 17 healthy men were divided into motion sickness sensitive group (12) and motion sickness insensitive groups (5) according to their endurance times and the severeness of symptom. Result. The composite equilibrium score significantly decreased post-swing in all subjects. The composite equilibrium score, certain other equilibrium scores (SOT4, SOT5 and SOT6), the strategy scores (STRAT4 and STRAT6) and the sensory score (SEN3 and SEN5) significantly decreased in the sensitive group, but unchanged in the insensitive group post-swing. The equilibrium score (SOT2), and the sensory score (SEN1) pre-swing, the strategy score (STRAT3) of insensitive group is significantly higher than the sensitive group. Conclusion. Motion sickness can influence postural equilibrium. Postural instability and instability of strategy are related to the sensitiveness to motion sickness.

[Comparison between motion sickness induced by reversed vision and that by parallel swing].

OBJECTIVE: To make clear the characteristics of motion sickness induced by reversed vision and to see whether it can be used in motion sickness experiments. METHODS: 10 healthy young men experienced walking tests wearing up-down or left-right reversing prisms, and parallel swing test on different days with intervals of 5-7d. Ataxia and motion sickness symptoms induced by walking wearing reversing prisms and those by parallel swing were observed. The reversed vision tolerance index (RVTI) and linear acceleration tolerance index (LATI) were calculated by an empirical formula. RESULTS: Both types of reversing prisms can induce obvious ataxia and motion sickness symptoms. The ataxia is correlative (P<0.05) with the susceptibility to motion sickness. In comparison with symptoms of swing motion sickness, the symptoms of reversed vision motion sickness are not too serious and appear slowly. There are correlativities (P< 0.01) between the susceptibility to motion sickness induced by up-down and left-right reversing prisms. The susceptibilities to motion sickness induced by reversing prisms are not correlative completely with that by swing. CONCLUSION: Reversed vision test is simple and easy and can be used for simulating space motion sickness or training of adaptation to sensory conflict on the ground.

[Recording and analysis of EGG and its application in space medicine].

To demonstrate the use of EGG in space medicine, especially in motion sickness, biological basis, recording, analysis of EGG used in space medicine, especially in motion sickness studies, were introduced. Several parameters of EGG were related to nausea in motion sickness. It is suggested that EGG can be used in studying nausea symptoms of motion sickness.

[Living in old age in Switzerland. Changes in the residence characteristics of the elderly population]. / Vivre sa vieillesse en Suisse. Les transformations des modes de residence des personnes agees.

PIP: "We analyse here the household situation of the elderly in Switzerland.... The situation at the beginning of the '90s is described in the different age groups of the second part of the lifecourse and in reference to individual profiles. In a second step, this information is considered in the light of the evolution which occurred during the last thirty years.... The objective is to examine in what measure elderly people are also affected by the new lifestyles shown in particular in the diversification of the household arrangements. More broadly, the aim is to contribute to a better understanding of the current mutations of the elderly's position in the family and in the society." (EXCERPT)^ieng