ABSTRACT
New Zeland Black (NZB) mice develop an autoimmune disease involving an abnormal B cell response to peripheral self Ags. This disease is associated with defects in other cell types and thymic stromal organization. We present evidence that NZB cells of various lineages, including thymocytes, fibroblasts, and dendritic precursor cells, show impaired proliferation and enhanced cell death in culture upon stimulation compared with non-autoimmune-prone mice such as C57BL/6. This phenotype explains the reduced efficiency of maturation of bone marrow-derived dendritic cells and the loss of TNF- or IL-1-dependent thymocyte costimulation. Upon TNF-induced activation of NZB thymocytes, nuclear translocation and DNA binding of RelA- and RelB-dependent NF-kappaB heterodimers are significantly reduced. This phenotype has a transcriptional signature, since the NZB, but not the nonobese diabetic, thymic transcriptome shows striking similarities with that of RelB-deficient thymuses. This partial NF-kappaB deficiency detected upon activation by proinflammatory cytokines could explain the disorganization of thymic microenvironments in NZB mice. These combined effects might reduce the efficiency of central tolerance and expose apoptotic debris generated during inflammatory processes to self recognition.
Subject(s)
Autoimmune Diseases/genetics , Dendritic Cells/pathology , Genetic Predisposition to Disease/genetics , NF-kappa B/deficiency , Proto-Oncogene Proteins/deficiency , Signal Transduction/genetics , Signal Transduction/immunology , T-Lymphocyte Subsets/pathology , Thymus Gland/pathology , Transcription Factors/deficiency , Active Transport, Cell Nucleus/genetics , Active Transport, Cell Nucleus/immunology , Animals , Autoimmune Diseases/metabolism , CD3 Complex/pharmacology , Cell Death/genetics , Cell Death/immunology , Cell Differentiation/genetics , Cell Differentiation/immunology , Cell Division/genetics , Cell Division/immunology , Cells, Cultured , Dendritic Cells/immunology , Embryo, Mammalian , Fibroblasts/immunology , Fibroblasts/pathology , Gene Library , Interleukin-1/pharmacology , Lymphocyte Activation/genetics , Mice , Mice, Inbred C57BL , Mice, Inbred NOD , Mice, Inbred NZB , Mice, Knockout , NF-kappa B/genetics , NF-kappa B/metabolism , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , T-Lymphocyte Subsets/immunology , Thymus Gland/embryology , Thymus Gland/immunology , Transcription Factor RelB , Transcription Factors/genetics , Transcription Factors/metabolism , Transcription, Genetic/immunology , Tumor Necrosis Factor-alpha/pharmacologyABSTRACT
Thymocyte maturation is controlled by successive developmental checkpoints connected to the acquisition of a functional T cell receptor (TCR). During thymocyte selection, engagement of the TCR regulates the fine balance between death and survival signals. At the final stages of single-positive (SP) thymocyte maturation, the coupling of the TCR changes from death- to proliferation-inducing signals, a competence required for optimal effector functions in the periphery. We show here that in RelB mutant thymuses, thymocyte differentiation of CD24(-) SP cells is partially impaired. Competitive bone marrow reconstitution experiments show that this defect is constitutive to the lymphoid compartment. This is accompanied by an increased proportion of apoptotic thymocytes and a drastically reduced proliferation upon activation with anti-CD3 antibody/PMA stimulation. Thus, the RelB protein contributes to the quality of cell signaling in thymocytes by providing anti-apoptotic signals. These results suggest that in addition to its major role on the activation of antigen-presenting cell function, the RelB protein is intrinsically required for terminal thymocyte differentiation and activation.
