Assessment of baseline ecotoxicity of sediments from a prospective mining area enriched in light rare earth elements.

Rare earth elements (REEs) disperse from indigenous rocks to the environment, thus making sediments one of the major sinks and sources of metal pollution. The emerging use of REEs and the subsequent opening of new mining areas may contribute to their release into surrounding ecosystems. For this reason, this study was performed in a natural area with geological material abundant in ferrocarbonatites and light REE. The aim of this work was to assess the natural REE availability and (eco)toxicity in freshwater sediments. Sediments showed high REE concentrations in samples with fine grain size fractions, and low in organic-rich sediments. The enrichment in LREE was mostly from rocks and the obtained enrichment factors (EF) confirmed that the sediments are not anthropogenically polluted. To assess REE availability and ecotoxicity, four toxicity tests were performed. REEs measured as the dissolved concentration in the test media were very low compared to the potentially available total REE in sediments and showed positive or negative correlations with fine or coarse grain sizes, respectively, and positive correlations with the content in Mg, Fe and Al. In tests performed in media supplemented with salts, the availability of REEs decreased considerably. Only some toxic effects could be linked to the REE contents in the ostracodtox and luminotox tests. However, measurement of toxicity could be influenced by the sediment properties and lead to a potential overestimation of ecotoxicity if only REE are regarded. Our study reveals that the physicochemical properties of sediments are a key factor controlling both REE availability and toxicity, whereas the determination of REE effects from toxicity tests using liquid media with salt addition will decrease REE availability and could mask toxic effects. Our findings provide new knowledge about REE behaviour in sediments and are a starting point for understanding potential REE pollution around prospective mining areas.

Phosphorus availability modulates the toxic effect of silver on aquatic fungi and leaf litter decomposition.

The functioning of forested headwater streams is intimately linked to the decomposition of leaf litter by decomposers, mainly aquatic hyphomycetes, which enables the transfer of allochthonous carbon to higher trophic levels. Evaluation of this process is being increasingly used as an indicator of ecosystem health and ecological integrity. Yet, even though the individual impacts of contaminants and nutrient availability on decomposition have been well studied, the understanding of their combined effects remains limited. In the current study, we investigated whether the toxic effects of a reemerging contaminant, silver (Ag), on leaf litter decomposition could be partly overcome in situations where microorganisms were benefitting from high phosphorus (P) availability, the latter being a key chemical element that often limits detritus decomposition. We also investigated whether these interactive effects were mediated by changes in the structure of the aquatic hyphomycete community. To verify these hypotheses, leaf litter decomposition by a consortium of ten aquatic hyphomycete species was followed in a microcosm experiment combining five Ag contamination levels and three P concentrations. Indirect effects of Ag and P on the consumption of leaf litter by the detritivorous crustacean, Gammarus fossarum, were also evaluated. Ag significantly reduced decomposition but only at the highest concentration tested, independently of P level. By contrast, P and Ag interactively affected fungal biomass. Both P level and Ag concentrations shaped microbial communities without significantly affecting the overall species richness. Finally, the levels of P and Ag interacted significantly on G. fossarum feeding rates, high [Ag] reducing litter consumption and low P availability tending to intensify the feeding rate. Given the high level of contaminant needed to impair the decomposition process, it is unlikely that a direct effect of Ag on leaf litter decomposition could be observed in situ. However, subtle Ag effects in relation to nutrient levels in ecosystems could be expected. In particular, owing to higher consumption of low P leaf litter, shredding invertebrates could increase the ingestion of contaminated resources, which could, in turn, represent an important threat to headwater stream ecosystems.

Behavioural and physiological responses of Gammarus fossarum (Crustacea Amphipoda) exposed to silver.

The study aims at investigating the effects of silver (Ag), a re-emerging contaminant, on physiological and behavioural responses in Gammarus fossarum. In a first experiment, G. fossarum Ag LC50s were evaluated during 96 h under semi-static mode of exposure. Juveniles appeared to be more sensitive to Ag (LC5096h: 1.01 µg L(-1)) than ovigerous females (LC5096h: 1.9 µg L(-1)) and adult males (LC5096h: 2.2 µg L(-1)). In a second experiment, the physiological (osmo-/ionoregulation; antioxidant enzymes; lipid peroxidation (LPO)) and behavioural (locomotor activity and ventilation) responses of male G. fossarum exposed to Ag (0, 0.5, 1, 2, and 4 µg L(-1)) were investigated. The mortality and Ag bioconcentration of gammarids exposed to Ag were significantly higher than controls. Concerning physiological responses, a 48 h-exposure to Ag had no impact on catalase activity but led to a significant decrease of haemolymph osmolality and [Na(+)]. On the contrary, LPO, Se-GPx and Na(+)/K(+)-ATPase activity were significantly increased. Behavioural responses, such as locomotor and ventilatory activities, were also significantly reduced in Ag exposed gammarids. After 96 h-exposure, especially to 0.5 µg Ag L(-1), most responses (ventilation, locomotor activity, haemolymph osmolality and [Na(+)]) were even more pronounced and haemolymph [Cl(-)] was significantly decreased but, contrary to observations after 48 h-exposure, Na(+)/K(+)-ATPase activity was significantly reduced. Our results demonstrate the drastic effects of realistic [Ag] concentration (0.5 µg Ag L(-1)) on an ubiquitous and functionally important freshwater invertebrate (implied in detritus breakdown), but also strongly suggest an energetic reallocation to the detriment of locomotor activity and in favour of maintenance functions (i.e., osmoregulation and detoxification). These results highlight the risk represented by Ag and the need to perform integrated studies (at different scales, from individual to ecosystem).

Evaluation of streamwater composition changes in the Vosges Mountains (NE France): 1955-2005.

In 1995, in the southwestern Vosges Mountains (NE France), 158 of 395 streams (40%) had a pH lower than 5.5 at baseflow. As elsewhere in Europe, acid deposition has decreased since the seventies, as has base cation deposition. In order to assess the response of streamwater to decreasing deposition, we compared their present chemical composition to their former composition. All comparisons showed a decrease in sulphate concentration, which was greater on granite than on sandstone. In addition calcium, magnesium and aluminium concentrations generally decreased. Acidity in streams draining granite decreased in spring, especially during the eighties; decreases were not observed on sandstone. Continuous monitoring of 5 streams since 1998 confirmed that Al concentrations decreased while changes in pH were small. Chemical trends in streams from the Vosges massif fell between those measured in Northern Europe and Central Europe. This study provides the first broad-scale overview of surface water acidification and recovery in France and emphasizes the need for continuous monitoring to assess long-term changes in aquatic ecosystems.

Elevated aluminium concentration in acidified headwater streams lowers aquatic hyphomycete diversity and impairs leaf-litter breakdown.

Aquatic hyphomycetes play an essential role in the decomposition of allochthonous organic matter which is a fundamental process driving the functioning of forested headwater streams. We studied the effect of anthropogenic acidification on aquatic hyphomycetes associated with decaying leaves of Fagus sylvatica in six forested headwater streams (pH range, 4.3-7.1). Non-metric multidimensional scaling revealed marked differences in aquatic hyphomycete assemblages between acidified and reference streams. We found strong relationships between aquatic hyphomycete richness and mean Al concentration (r = -0.998, p < 0.0001) and mean pH (r = 0.962, p < 0.002), meaning that fungal diversity was severely depleted in acidified streams. By contrast, mean fungal biomass was not related to acidity. Leaf breakdown rate was drastically reduced under acidic conditions raising the issue of whether the functioning of headwater ecosystems could be impaired by a loss of aquatic hyphomycete species.

Physiological recovery from episodic acid stress does not mean population recovery of Gammarus fossarum.

The physiological responses of the acid-sensitive amphipod Gammarus fossarum exposed in situ to acid stress (pH4.5 and 5.5) and then transferred back to neutral water were investigated. Survival rate and haemolymph [Cl(-)] and [Na(+)] were assessed after 24, 48 and 72h of exposure in acidic streams and after a recovery period of 12, 24, 36, 48 and 60h. After 24h, exposure to slightly acidic (pH5.5) and strongly acidic water (pH4.5) led to a severe and significant depletion in haemolymph [Na(+)] and [Cl(-)] compared to organisms exposed in circumneutral water (pH7.3). However, after only a 12h-period of transfer back in neutral water and whatever the previous exposure time (24, 48 and 72h) in both slightly and strongly acidic water, haemolymph [Na(+)] and [Cl(-)] were equal or superior to the control level without associated mortality. In spite of this fast physiological recovery capacity, populations of G. fossarum living in streams undergoing episodic acid stresses were drastically affected thus, demonstrating the high acid-sensitivity of this species. We discuss the possible reasons of population regression and the absence of population recovery.

Short-term physiological responses to a severe acid stress in three macroinvertebrate species: a comparative study.

The present study focuses on the sensitivity among freshwater invertebrate species to acidic stress. Three common macroinvertebrate species in the Vosges Mountains (North-Eastern France), Gammarus fossarum (Amphipoda), Hydropsyche pellucidula (Trichoptera) and Dinocras cephalotes (Plecoptera) were exposed for 24, 72 and 120 h to natural acidified water (pH=4.73+/-0.08, [Ca2+]=39.1+/-0.6 micromol l(-1), [Al(tot)]=28.4+/-1 micromol l(-1)). Short-term exposure to acid stress caused significant decreases both in survival rate and haemolymph ions ([Cl-] and [Na+]). The relative sensitivity to a natural acidic stress slightly differed among the species and was in the following order: G. fossarum, as the most sensitive, then H. pellucidula and D. cephalotes. Results of this study confirm the interest of in situ tests to assess the toxicity of short-term acid exposure. Finally, our results reinforce the hypothesis that transient acidification can offset the recovery of sensitive species of macroinvertebrates in streams chemically recovering from acidification either through liming or declining deposition.

Hyperventilation and loss of hemolymph Na+ and Cl- in the freshwater amphipod Gammarus fossarum exposed to acid stress: a preliminary study.

The effect of acidification on the acid-sensitive species Gammarus fossarum was investigated in the laboratory. The results showed that as mortality increased, mean hemolymph chloride and sodium concentrations decreased rapidly. Concomitantly, organisms hyperventilated during the first 24 h and then started to hypoventilate. These results demonstrated that exposure to acid stress in the acid-sensitive species G. fossarum led to ion-regulatory and respiratory failure as previously reported in fish and crayfish exposed to acid stress.