Erxian decoction alleviates cisplatin-induced premature ovarian failure in rats by reducing oxidation levels in ovarian granulosa cells.

ETHNOPHARMACOLOGICAL RELEVANT: Erxian Decoction (EXD) has been used empirically for more than 70 years to treat premature ovarian failure (POF), but more research is needed to understand how it works. AIM OF THE RESEARCH: The study aims to ascertain both in vivo and in vitro rewards of EXD. MATERIALS AND METHODS: EXD is composed of Curculiginis Rhizoma, Epimedii Folium, Morindae Officinalis, Angelicae Sinensis, Anemarrhenae Rhizoma, and Phellodendri Chinensis Cortex. UPLC/MS analysis was used to investigate the components of EXD. Using a POF model created by administering cisplatin to rats intraperitoneally, the pharmacodynamic effects of EXD were investigated. Three dose groups of EXD were garaged into rats: high (15.6 g/kg), medium (7.8 g/kg), and low (3.9 g/kg). By using a vaginal smear, the impact of EXD on the rat estrous cycle was evaluated. An ELISA test was used to measure the anti-Mullerian hormone (AMH), estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels in the serum of rats. By using HE stains, pathological alterations in the ovaries may be seen. MDA and SOD levels in ovarian samples were used to measure the degree of ovarian oxidation. TUNEL labeling of ovarian sections was used to find apoptosis levels. By using ATP, energy production was evaluated. The relative expression of proteins connected to aging and the RAGE pathway was assessed using Western blot. Then, using H2O2, a model of senescent human ovarian granulosa cells (KGN) was created in vitro. The impact of EXD and H2O2 on cellular senescence was discovered using-galactosidase staining. Cell apoptosis levels were found using PI/Hoechest33342. By using DCFH-DA, intracellular ROS was examined. MDA and SOD concentrations were used to measure the degree of cellular oxidation. RAGE-related mRNA and protein expression were evaluated using RT-qPCR and western blotting. RESULTS: Using UPLC/MS analysis, 39 chemicals in EXD were found. Rats' estrous cycles were enhanced by EXD, which increased ovarian index and follicle count and reduced the proportion of atretic follicles in the rats. EXD reduced LH and FSH output while restoring AMH and E2 secretion. In ovarian tissues, EXD reduced the amount of apoptosis and MDA while raising SOD activity and ATP levels. The protein levels of p16, p21, p53, and Lamin A/C were among the senescence-related proteins that EXD lowered, along with the levels of RAGE, PI3K, BAX, and CASPASE 3. Anti-apoptotic protein BCL-2 was also raised in the RAGE pathway. Senescence, apoptosis, ROS, and MDA levels in the KGN cells were lowered in vitro by EXD. Additionally, EXD increased the anti-apoptotic potential by changing the expression of CAT, SOD2, and SIRT1. RAGE, BAX, BCL-2, CASPASE 3, and p38 expression levels were altered by EXD, enhancing its anti-apoptotic capability. CONCLUSION: EXD boosted the ovary's antioxidant and anti-apoptotic capabilities while enhancing the estrous cycle and hormone output. These findings strongly suggested that EXD may contribute to the alleviation of POF and ovarian granulosa cells senescence.

Nitrogen Self-Doping Carbon Derived from Functionalized Poly(Vinylidene Fluoride) (PVDF) for Supercapacitor and Adsorption Application.

A new synthetic strategy has been developed for the facile fabrication of a N-doped porous carbon (NC-800) material via a facile carbonization of functionalized poly(vinylidene fluoride) (PVDF). The prepared NC-800 exhibits good specific capacitance of 205 F/g at 1 A/g and cycle stability (95.2% retention after 5000 cycles at 1 A/g). The adsorption capacity of NC-800 on methylene blue and methyl orange was 780 mg/g and 800 mg/g, respectively. The facile and economical method and good performance (supercapacitor and adsorption) suggest that the NC-800 is a promising material for energy storage and adsorption.

Mango-Stone-Derived Nitrogen-Doped Porous Carbon for Supercapacitors.

The preparation of N-doped porous carbon (NC-800) is presented via facile mango stone carbonization at 800 °C. The NC-800 material exhibits good cycle stability (the capacity retention is 97.8% after 5000 cycles) and high specific capacitance of 280 F/g at 1 A/g. Furthermore, the assembled symmetric device of NC-800//NCs-800 exhibits about 31.1 Wh/kg of energy density at 800 W/kg in a voltage range of 0-1.6 V. The results of the study suggest that NC-800 may be a promising energy storage material for practical application.