ABSTRACT
OBJECTIVE: To investigate the relationship between transforming growth factor beta-1 (TGF-ß(1)) gene-509C/T polymorphism and severe chronic periodontitis in Chinese Hans population. METHODS: TGF-ß(1)-509C/T genotype polymorphism was analyzed in 102 patients with severe chronic periodontitis (periodontitis group) and 102 healthy controls (control group) by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: The distributions of TGF-ß(1)-509C/T genotype and allele were significantly different between severe chronic periodontitis group and control group (P < 0.05). TGF-ß(1)-509CC, CT and TT genotype frequency were 44.1% (45/102), 47.1% (48/102), 8.8% (9/102) in periodontitis group and 29.4% (30/102), 51.0% (52/102), 19.6% (20/102) in control group, respectively. The relative risk analysis found that C allele carriers had higher risk of suffering from severe chronic periodontitis compared with T allele carriers (OR = 1.718, 95%CI: 1.148-2.569). CONCLUSIONS: TGF-ß(1)-509C/T polymorphism is associated with severe chronic periodontitis in Chinese Hans population, and C allele may be an important genetic susceptibility gene for severe chronic periodontitis.
Subject(s)
Chronic Periodontitis/genetics , Genetic Predisposition to Disease , Polymorphism, Genetic , Transforming Growth Factor beta1/genetics , Alleles , Genotype , Humans , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Transforming Growth Factor betaABSTRACT
OBJECTIVE: To investigate the relationship between interleukin-6 (IL-6) gene -572C/G polymorphism and the risk of chronic periodontitis disease. METHODS: IL-6-572C/G genotype polymorphism was examined in 93 patients with chronic periodontitis and 96 control subjects by PCR restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: IL-6-572GG genotype and G allele were more frequent in chronic periodontitis patients than that in control subjects (P<0.05). -572CC, CG and GG genotype frequency were 52.7 % , 40.9 %, 6.4 % in chronic periodontitis patients and 67.7 %, 31.3%, 1.0 % in control subjects, respectively. -572CG + GG genotype and G allele were more frequent in patients than that in control subjects (P<0.05). Compared with CC genotype,the odds ratio for chronic periodontitis was 1.88 (95% CI: 1.04-3.40, P<0.05) for CG + GG genotype. CONCLUSIONS: The frequency of -572CG + GG genotype was higher in patients with chronic periodontitis compared with the control subjects. IL-6-572C/G polymorphism may be a genetic susceptibility factor for chronic periodontitis in Chinese Hans population.
