[Somaclonal variability of sugarbeet plants on the basis of pollen sterility]. / Somaklonal'naia izmenchivost' rastenii sakharnoi svekly po priznaku steril'nosti pyl'tsy.

The nature of pollen sterility in sugarbeet regenerated plants obtained from callus cultures of inbred lines has been investigated. It has been shown that detected male sterility of plants can be caused both by epigenetic and mutation factors. The forms with cytoplasmic and nuclear sterility have been selected.

[Characteristics of lipopolysaccharide from Pseudomonas fluorescens (biovar I)]. / Kharakteristika lipopolisakharida Pseudomonas fluorescens (biovar I).

Lipopolysaccharide (LPS) of the Pseudomonas fluorescens strain IMV 7769 (biovar I) was isolated and investigated. Fractions of the structural parts of the LPS macromolecule, lipid A, the core oligosaccharide, and the O-specific polysaccharide (O-PS), were obtained in a homogeneous state. 2-Hydroxydecanoic, 3-hydroxydecanoic, dodecanoic, 2-hydroxydodecanoic, 3-hydroxydodecanoic, hexadecanoic, octadecanoic, hexadecenoic, and octadecenoic fatty acids were identified in lipid A. In the hydrophilic moiety of lipid A, after acid hydrolysis, several amino acids, phosphoethanolamine, glucosamine, and three unidentified peaks forming a separate cluster together with glucosamine were found. Lipid A was shown to be phosphorylated. Glucose, fucose, rhamnose, glucosamine, galactosamine, two unidentified amino sugars, 2-keto-3-deoxyoctulonic acid (KDO), heptose, ethanolamine, phosphoethanolamine, and alanine were identified in the core oligosaccharide. O-PS of the LPS consisted of repeating trisaccharide fragments that included residues of amino sugars: 4-acetamido-4,6-dideoxy-D-galactose, 2-acetamido-2,6-dideoxy-D-glucose, and 2-acetamido-2,6-dideoxy-L-glucose. During growth, the strain under study excreted exocellular LPS (ELPS) into the medium. The LPS studied was similar to the LPS of the earlier investigated strains P. fluorescens (biovar I) IMV 1152 and IMV 1433 in the structure of O-PS, but differed from them in the composition of both lipid A and the core oligosaccharide. The LPS of the strain studied differed from LPS of the type strain P. fluorescens IMV 4125 (ATCC 13525) in all characteristics determined.

Characterization of lipopolysaccharides from Pseudomonas syringae (serogroup II).

Lipopolysaccharides (LPS) from the strains of Pseudomonas syringae pv.syringae 90a, 435, pv. atrofaciens K-1025, pv. morsprunorum CF-4 referred by Pastushenko and Simonovich (1979) to serogroup II have been studied. The strains were shown to be heterogeneous by chemical composition of core and lipid A and structure of O-specific polysaccharide. The preparations heterogeneity in serological cross reactions were also detected. O-specific polysaccharides of the strains having similar structures were not identical in serological tests. The assumption on the lipid A role in serogrouping of P. syringae strains has been advanced.

[The characteristics of the lipid A of the lipopolysaccharides in Pseudomonas syringae strains]. / Kharakteristika lipida A lipopolisakharidov shtammov Pseudomonas syringae.

Component composition of lipid A of Pseudomonas syringae pv. syringae 218 and P-55, pv. syringae (holci) 8299, pv. phaseolicola 120a, pv. atrofaciens 2399 has been studied. The lipid A composition of all the strains studied includes 3-hydroxydecanoic fatty acid (3-OH-C10:0), 2-hydroxydodecanoic (2-OH-C12:0), 3-hydroxydodecanoic (3-OH-C12:0), dodecanoic (C12:0), hexadecanoic (C16:0), octadecanoioc (C18:0), hexadecenic (C16:1), octadecenic (C18:1) fatty acids. The carbohydrate part of the lipid A macromolecule of all strains after acid hydrolysis contains ethanolamine, phosphoethanolamine, glucosamine.

[The immunochemical characteristics of the lipopolysaccharides of Pseudomonas syringae (pathovars atrofaciens and phaseolicola) and P. holci (serogroup VI)]. / Immunokhimicheskaia kharakteristika lipopolisakharidov Pseudomonas syringae (patovarov atrofaciens, phaseolicola) i P. holci (VI serogruppa).

Lipopolysaccharides (LPS) of the representatives of strains of serogroup VI Pseudomonas syringae (P. syringae pv. atrofaciens 2399, pv. phaseolicola 120a, 7842 and P. holci 8299) possessing virulence and confinement to the host-plant are characterized by high serological activity in direct and cross reactions of the binary diffusion in agar, immunoelectrophoresis, passive hemagglutination and inhibition of passive hemagglutination. A supernatant and a sediment obtained after ultracentrifugation of LPS preparations possessed O-antigenic activity. O-specific polysaccharide (PS) is serologically less active than the LPS preparations. Problems of the intergroup and intragroup serological affinity in connection with the structure of O-specific PS. It is proved that the basic chain of O-specific polysaccharide (D-rhamnane) plays definite (but not a single) part in displaying antigenic properties of the whole LPS macromolecule.

[Antigenic bacterial polysaccharides. 29. The structure of the polysaccharide chain of Pseudomonas holci 8300 (serogroup I) lipopolysaccharide]. / Antigennye polisakharidy bakterii. 29. Struktura polisakharidnoi tsepi lipopolisakharida Pseudomonas holci 8300 (serogruppa I).

The Pseudomonas holci 8300 lipopolysaccharide has an O-specific polysaccharide chain, containing L-rhamnose and 3-acetamido-3-deoxy-D-fucose residues in the ratio 4:1. On the basis of methylation, Smith degradation, and 1H- and 13C-NMR spectroscopy data, it was concluded that the polysaccharide is built up of pentasaccharide units of A and B types in the ratio approximately 2.5:1. In some stretches of the polysaccharide, minor B units form rather long chains, and in the others they alternate with predominant A units. (formula; see text)

[Antigenic bacterial polysaccharides. 30. The structure of the polysaccharide chain of lipopolysaccharide of Pseudomonas syringae pv. syringae 281 (serogroup I)]. / Antigennye polisakharidy bakterii. 30. Struktura polisakharidnoi tsepi lipopolisakharida Pseudomonas syringae, patovar syringae 281 (serogruppa I).

Anomeric methyl 3-O-(D-mannopyranosyl- and L-rhamnopyranosyl)-beta-D-talopyranosides were synthesised by the stereoselective 1,2-cis- and 1,2-trans manno- and rhamnosylation of methyl 2,4,6-tri-O-acetyl-beta-D-talopyranoside, which has been prepared from methyl beta-D-galactopyranoside by a synthetic scheme including conversion of the C2 configuration. From 13C-NMR spectra of the disaccharides obtained the spectral alpha- and beta-effects of O3-glycosylation of talopyranose were determined.

[Antigenic polysaccharides of bacteria. 27. Structure of the O-specific polysaccharide chain of lipopolysaccharides from Pseudomonas syringae pv. atrofaciens 2399, phaesolica 120a and Pseudomonas holci 8299 belonging to serotype VI]. / Antigennye polisakharidy bakterii. 27. Stroenie O-spetsificheskoi polisakharidnoi tsepi lipopolisakharidov Pseudomonas syringae, patovary atrofaciens 2399, phaesolicola 120a, i Pseudomonas holci 8299, otnosiashchikhsia k serogruppe VI.

Lipopolysaccharides from Pseudomonas syringae pvs atrofaciens 2399. phaseolicola 120a and Pseudomonas holci 8299, belonging to serogroup VI. possess an identical polysaccharide chain composed of D-rhamnose and D-fucose. On the hasis of methylation, partial acid hydrolysis, 1H- and 13C-NMR data, it was concluded that the backbone of the polysaccharide represents D-rhamnan built up of tetrasaccharide repeating units and alpha-D-fucofuranose residues are attached to the backbone as the monosaccharide branches. The following structure of the repeating unit is established: (Formula: see text).