Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts.

Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblastoid nature of leptomeningeal cells and their ability to form a sheet-like layer that covers the brain and spine parenchyma. These findings will enable researchers in central nervous system barriers to describe leptomeningeal cell functions in health and disease.

Synthesis of an Alkynyl Methylglyoxal Probe to Investigate Nonenzymatic Histone Glycation.

Methylglyoxal (MGO) is a reactive dicarbonyl metabolite that modifies histones in vivo and induces changes in chromatin structure and function. Here we report the synthesis and application of a chemical probe for investigating MGO-glycation. A two-step synthesis of a Cu-click compatible alkynyl oxoaldehyde probe (AlkMGO) via sequential Dess-Martin and Riley oxidations is presented. This synthesis elevates the accessibility and utility of an important tool for tracking, enriching, and studying MGO-glycation to aid in understanding its underlying biochemical functions.

El estado de ánimo precompetitivo en un equipo de fútbol profesional: Un estudio entre jugadores titulares y suplentes / The players´mood in a professional soccer team: a study between regular players and substitutes

El objetivo de este artículo se centra en profundizar en la importancia que tiene el estado anímico de los jugadores de un equipo de fútbol profesional, abordando su estudio desde un enfoque novedoso como es la comparación de jugadores titulares y suplentes.. La muestra la componen los jugadores de la AD Alcorcón de la Segunda División B del fútbol español (Madrid, España), que fueron evaluados mediante la versión reducida del Profile of Mood State (POMS, MacNair, Lorr and Droppelmann, 1971), en la versión de 29 ítems (Fuentes, García-Merita, Meliá and Balaguer, 1995), durante cinco partidos de la temporada regular -inmediatamente anteriores a la disputa de la fase de ascenso a 2ª División A-. Mediante la prueba no paramétrica para muestras independientes U de Mann-Whitney, se pone de manifiesto que existen diferencias significativas en dos de las cinco escalas del POMS, en concreto, en la escala de cólera y depresión. Los resultados permiten reflexionar sobre las estrategias de optimización del rendimiento psicológico de los jugadores titulares y suplentes en momentos clave de la temporada, ayudando a los entrenadores a crear las condiciones para mejorar las puntuaciones ofrecidas por jugadores que se alejan del perfil ideal “iceberg” (Morgan, 1980a, 1980 b) (AU)

The aim of this paper focuses on determining the importance of the players´mood in a professional football team to study the differences between regular players and substitutes. The sample consisted of players from the AD Alcorcón, a third division team of the Spanish football league (Madrid, Spain), which were evaluated using the short version of the Profile of Mood State (POMS, McNair, Lorr and Droppelmann, 1971), in version of 29 items (Fuentes, García-Merita, Meliá and Balaguer, 1995), for five regular season games, immediately prior to the competition on the stage of promotion to Division 2 A-. By means of a nonparametric test for independent samples Mann-Whitney, significant differences are revealed in two of the five scales of the POMS, in particular, on the scale of anger and depression (..) (AU)

O objetivo desse artigo foi de aprofundar na importância que tem o estado de animo dos Jogadores (..) (AU)