Subject(s)
Dermatomycoses/veterinary , Dog Diseases/diagnosis , Microsporum/immunology , Animals , Antifungal Agents/therapeutic use , Dermatomycoses/diagnosis , Diagnosis, Differential , Dog Diseases/blood , Dog Diseases/drug therapy , Dog Diseases/microbiology , Dogs , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Immunoglobulin G/immunology , Male , Microsporum/isolation & purification , Predictive Value of TestsABSTRACT
We describe a case of a 3-month-old Persian male cat with a persistent waxy, ceruminous, otic discharge in both external auditory canals. After microbiological culture, only Microsporum canis was isolated with no concurrent fungal or bacterial flora. Fungal otitis due to dermatophytes was diagnosed and specific treatment with griseofulvin was administered. After 4 weeks of treatment, no M. canis colonies were isolated and clinical recovery had occurred. In view of this case, dermatophytosis should be included in the differential diagnosis of feline otic conditions, particularly when otitis becomes chronic or is refractory to treatment.
Subject(s)
Cat Diseases/etiology , Microsporum/isolation & purification , Otitis Externa/veterinary , Animals , Cat Diseases/diagnosis , Cats , Male , Otitis Externa/diagnosis , Otitis Externa/etiologyABSTRACT
Samples from otitis externa in the dog were cultured on a variety of media under different conditions of incubation in order to assess the most suitable conditions, especially for the isolation of Malassezia pachydermatis. Columbia blood and MacConkey agar at 37 degrees C for 24 h with air and Sabouraud glucose agar under microaerophilic conditions for 48 h at 37 degrees C were considered the ideal combination.
Subject(s)
Dermatomycoses/veterinary , Dog Diseases/microbiology , Malassezia/isolation & purification , Otitis Externa/veterinary , Animals , Cell Culture Techniques , Culture Media , Dermatomycoses/microbiology , Dogs , Malassezia/physiology , Otitis Externa/microbiologyABSTRACT
In this paper we present the results of studies on the susceptibility to antibiotics of bacteria isolated from chronic canine otitis externa. We tested 46 bacterial strains (S. aureus, coagulase-negative staphylococci, Corynebacterium spp., and gram-negative bacilli) with 20 different antibiotics. We observed increased resistance to antibiotics of bacteria isolated from canine otitis externa as compared to the resistance reported earlier. This may be due to the indiscriminate use of some antibiotics in the last years and indicates the importance of sensitivity testing for the effective treatment of chronic otitis externa, especially that caused by gram-negative bacilli. The clinician may initiate empiric treatment with antibiotics before obtaining the sensitivity test results; the best results may be expected from a topical application of Bacitracin or Chloramphenicol, and from a systemic therapy with Cephalosporines. Therapeutical scheme for treating various bacterial groups are presented in the paper.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Infections/veterinary , Dog Diseases/microbiology , Otitis Externa/veterinary , Animals , Bacterial Infections/microbiology , Chronic Disease , Corynebacterium/drug effects , Corynebacterium/isolation & purification , Dogs , Gram-Negative Bacteria/drug effects , Gram-Negative Bacteria/isolation & purification , Microbial Sensitivity Tests , Otitis Externa/microbiology , Staphylococcus/drug effects , Staphylococcus/isolation & purification , Staphylococcus aureus/drug effects , Staphylococcus aureus/isolation & purificationABSTRACT
Aspergillosis comprises a wide range of clinical conditions, of which the most serious is invasive aspergillosis, which particularly affects immunodeficient individuals. In the present work we present a review centred on three main aspects of this disease: 1) Possibility of differentiation of strains of Aspergillus fumigatus by phenotyphic and genotypic methods, 2) Mechanisms of pathogenicity of this species and especially the relationships of elastase activity, and 3) Laboratory diagnosis of aspergillosis, principaly in terms of determination of circulating antigens and DNA sequences in the blood and urine of patients.
ABSTRACT
It is important to test the antimicrobial sensitivity of the aetiological agents of otitis externa before starting treatment in order to prevent the development of antibiotic resistance in the microorganisms. In this study the sensitivity patterns in bacteria and yeasts isolated as aetiological agents from otitis externa were analysed. Antifungal and antibacterial activity tests were done using the automated system ATB Fungus and the classic diffusion test respectively. The azole compounds had the greatest antifungal properties, while beta-lactams had the strongest antibiotic activity. This study reflects the increasing importance of sensitivity tests in chronic otitis externa due to the increasing antimicrobial resistances in bacteria and yeasts. If it is necessary to initiate an empiric treatment before the results of the antimicrobial test, the application of miconazole or econazole for yeasts and oxacillin or amoxicillin-clavulanic acid against bacteria is recommended.
Subject(s)
Antifungal Agents/pharmacology , Dog Diseases/microbiology , Otitis Externa/veterinary , Amphotericin B/pharmacology , Animals , Dogs , Econazole/pharmacology , Miconazole/pharmacology , Microbial Sensitivity Tests/veterinary , Nystatin/pharmacology , Otitis Externa/microbiologyABSTRACT
The microbiological characteristics of otic exudates from 26 dogs with chronic otitis externa was studied with special reference to the implication of yeasts in the aetiology of the disease. A high frequency of yeasts and Staphylococcus aureus were isolated, alone or in association. In reference to the yeasts, there was a clear predominance of the genus Candida (48% of the total yeasts). Malassezia (Pytirosporum) represented only 3% of the isolates. It can be concluded that yeasts have an important role in the pathogenicity of this disease. For the microbiological diagnosis of otitis externa, we recommend the simultaneous use of Columbia/5% Sheep Blood Agar and Sabouraud-Dextrose without antibiotic addition, the use of 37 degrees C as the incubation temperature and direct microscopic observation of the sample before culture.
