ABSTRACT
Long chain bases (LCB) are both precursors of complex sphingolipids (SL) and cellular signals in eukaryotic cells. Increasing evidence support a function for SL and/or LCBs in plant responses to environmental cues. In this study we analysed the impact of a short exposure to cold on the global LCB content and composition in Arabidopsis thaliana seedlings. We report that the total LCB amount significantly decreased after low temperature exposure. The decline was essentially due to reduction of t18:1 isomer content. On the other hand, chilling led to the increase of LCB content in a mutant over-expressing the non-symbiotic haemoglobin AHb1. Furthermore, this mutant was impaired in cold-dependent root growth inhibition and anthocyanin synthesis. As AHb1 is an element of nitric oxide turnover, our data suggest a possible link between nitric oxide, SL content and cold stress response.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Hemoglobins/metabolism , Sphingolipids/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Cold Temperature , Hemoglobins/geneticsABSTRACT
In Arabidopsis, gene expression studies and analysis of knock-out (KO) mutants have been instrumental in building an integrated view of disease resistance pathways. Such an integrated view is missing in rice where shared tools, including genes and mutants, must be assembled. This work provides a tool kit consisting of informative genes for the molecular characterization of the interaction of rice with the major fungal pathogen Magnaporthe oryzae. It also provides for a set of eight KO mutants, all in the same genotypic background, in genes involved in key steps of the rice disease resistance pathway. This study demonstrates the involvement of three genes, OsWRKY28, rTGA2.1 and NH1, in the establishment of full basal resistance to rice blast. The transcription factor OsWRKY28 acts as a negative regulator of basal resistance, like the orthologous barley gene. Finally, the up-regulation of the negative regulator OsWRKY28 and the down-regulation of PR gene expression early during M. oryzae infection suggest that the fungus possesses infection mechanisms that enable it to block host defences.
Subject(s)
Databases, Genetic , Disease Resistance/genetics , Genes, Plant/genetics , Mutation/genetics , Oryza/genetics , Oryza/immunology , Plant Immunity/genetics , Gene Expression Regulation, Plant , Genome, Plant/genetics , Mutagenesis, Insertional/genetics , Phenotype , Plant Diseases/genetics , Plant Diseases/immunology , Plant Immunity/immunology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Transcription, GeneticABSTRACT
Miniature Inverted-repeat Transposable Elements (MITEs) are small nonautonomous class-II transposable elements distributed throughout eukaryotic genomes. We identified a novel family of MITEs (named Alex) in the Coffea canephora genome often associated with expressed sequences. The Alex-1 element is inserted in an intron of a gene at the CcEIN4 locus. Its mobility was demonstrated by sequencing the insertion site in C. canephora accessions and Coffea species. Analysis of the insertion polymorphism of Alex-1 at this locus in Coffea species and in C. canephora showed that there was no relationship between the geographical distribution of the species, their phylogenetic relationships, and insertion polymorphism. The intraspecific distribution of C. canephora revealed an original situation within the E diversity group. These results suggest possibly greater gene flow between species than previously thought. This MITE family will enable the study of the C. canephora genome evolution, phylogenetic relationships, and possible gene flows within the Coffea genus.
