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1.
J Diabetes Res ; 2022: 8566970, 2022.
Article in English | MEDLINE | ID: mdl-35967127

ABSTRACT

Aims: Mitochondrial dysfunction contributes to many forms of peripheral and central nervous system degeneration. Therapies that protect mitochondrial number and function have the potential to impact the progression of conditions such as diabetic neuropathy. We therefore assessed indices of mitochondrial function in dorsal root ganglia (DRG) and brain cortex of the Zucker diabetic fatty (ZDF) rat model of type 2 diabetes and tested the therapeutic impact of a neurogenic compound, NSI-189, on both mitochondrial function and indices of peripheral and central neurological dysfunction. Materials and Methods: ZDF rats were maintained for 16 weeks of untreated diabetes before the start of oral treatment with NSI-189 for an additional 16 weeks. Nerve conduction velocity, sensitivity to tactile and thermal stimuli, and behavioral assays of cognitive function were assessed monthly. AMP-activated protein kinase (AMPK) phosphorylation, mitochondrial protein levels, and respiratory complex activities were assessed in the DRG and brain cortex after 16 weeks of treatment with NSI-189. Results: Treatment with NSI-189 selectively elevated the expression of protein subunits of complexes III and V and activities of respiratory complexes I and IV in the brain cortex, and this was accompanied by amelioration of impaired memory function and plasticity. In the sensory ganglia of ZDF rats, loss of AMPK activity was ameliorated by NSI-189, and this was accompanied by reversal of multiple indices of peripheral neuropathy. Conclusions: Efficacy of NSI-189 against dysfunction of the CNS and PNS function in type 2 diabetic rats was accompanied by improvement of mitochondrial function. NSI-189 exhibited actions at different levels of mitochondrial regulation in central and peripheral tissues.


Subject(s)
Diabetes Mellitus, Experimental , Diabetes Mellitus, Type 2 , Diabetic Neuropathies , AMP-Activated Protein Kinases/metabolism , Aminopyridines , Animals , Diabetes Mellitus, Experimental/metabolism , Diabetes Mellitus, Type 2/metabolism , Mitochondria/metabolism , Piperazines , Rats , Rats, Zucker
2.
Clin Exp Allergy ; 47(5): 639-655, 2017 May.
Article in English | MEDLINE | ID: mdl-28093832

ABSTRACT

BACKGROUND: Interleukin-15 is a pleiotropic cytokine that is critical for the development and survival of multiple haematopoietic lineages. Mice lacking IL-15 have selective defects in populations of several pro-allergic immune cells including natural killer (NK) cells, NKT cells, and memory CD8+ T cells. We therefore hypothesized that IL-15-/- mice will have reduced inflammatory responses during the development of allergic airway disease (AAD). OBJECTIVE: To determine whether IL-15-/- mice have attenuated allergic responses in a mouse model of AAD. METHODS: C57BL/6 wild-type (WT) and IL-15-/- mice were sensitized and challenged with ovalbumin (OVA), and the development of AAD was ascertained by examining changes in airway inflammatory responses, Th2 responses, and lung histopathology. RESULTS: Here, we report that IL-15-/- mice developed enhanced allergic responses in an OVA-induced model of AAD. In the absence of IL-15, OVA-challenged mice exhibited enhanced bronchial eosinophilic inflammation, elevated IL-13 production, and severe lung histopathology in comparison with WT mice. In addition, increased numbers of CD4+ T and B cells in the spleens and bronchoalveolar lavage (BAL) were also observed. Examination of OVA-challenged IL-15Rα-/- animals revealed a similar phenotype resulting in enhanced airway eosinophilia compared to WT mice. Adoptive transfer of splenic CD8+ T cells from OVA-sensitized WT mice suppressed the enhancement of eosinophilia in IL-15-/- animals to levels observed in WT mice, but had no further effects. CONCLUSION AND CLINICAL RELEVANCE: These data demonstrate that mice with an endogenous IL-15 deficiency are susceptible to the development of severe, enhanced Th2-mediated AAD, which can be regulated by CD8+ T cells. Furthermore, the development of disease as well as allergen-specific Th2 responses occurs despite deficiencies in several IL-15-dependent cell types including NK, NKT, and γδ T cells, suggesting that these cells or their subsets are dispensable for the induction of AAD in IL-15-deficient mice.


Subject(s)
Allergens/toxicity , Asthma/immunology , CD8-Positive T-Lymphocytes/immunology , Interleukin-15/deficiency , Th2 Cells/immunology , Animals , Asthma/chemically induced , Asthma/genetics , Asthma/pathology , CD8-Positive T-Lymphocytes/pathology , Interleukin-15/immunology , Mice , Mice, Knockout , Natural Killer T-Cells/immunology , Natural Killer T-Cells/pathology , Th2 Cells/pathology
3.
Clin Exp Allergy ; 44(4): 589-601, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24397722

ABSTRACT

BACKGROUND: Natural Killer (NK) cells have been implicated in the development of allergic airway inflammation. However, the in vivo role of NK cells has not been firmly established due to the lack of animal models with selective deficiencies in NK cells. OBJECTIVE: To determine the specific contribution of NK cells in a murine model of allergic airway disease (AAD). METHODS: The role of NK cells in AAD was studied using NK-deficient (NKD) mice, perforin(-/-) mice, and mice depleted of Ly49A/D/G(+) NK cell subsets in an ovalbumin-induced model of allergic airway disease (OVA-AAD). RESULTS: Induction of OVA-AAD in C57BL/6 wild-type (WT) mice resulted in the expansion of airway NK cells and the development of pronounced airway eosinophilia. In the absence of NK cells or specific subsets of NK cells, either in NKD mice, or after the depletion of Ly49A/D/G(+) NK cells, the development of OVA-AAD was significantly impaired as seen by decreased airway inflammation and eosinophilia, decreased secretion of the Th2 cytokines IL-4, IL-5 and IL-13 and diminished OVA-specific antibody production. Furthermore, while OVA-exposure induced a dramatic expansion of dendritic cells (DCs) in WT mice, their induction was significantly attenuated in NKD mice. Development of OVA-AAD in perforin(-/-) mice suggested that the proinflammatory role of NK cells is not dependent on perforin-mediated cytotoxicity. Lastly, induction of allergic disease by OVA-specific CD4 T cells from WT but not NK-depleted or NKD mice in RAG(-/-) recipients, demonstrates that NK cells are essential for T cell priming. CONCLUSIONS AND CLINICAL RELEVANCE: Our data demonstrate that conventional NK cells play an important and distinct role in the development of AAD. The presence of activated NK cells has been noted in patients with asthma. Understanding the mechanisms by which NK cells regulate allergic disease is therefore an important component of treatment approaches.


Subject(s)
Killer Cells, Natural/immunology , Respiratory Hypersensitivity/immunology , Adoptive Transfer , Animals , Cytotoxicity, Immunologic , Dendritic Cells/immunology , Dendritic Cells/metabolism , Disease Models, Animal , Eosinophilia/immunology , Inflammation/immunology , Killer Cells, Natural/metabolism , Lung/immunology , Lung/pathology , Lymphocyte Activation/immunology , Mice , Mice, Knockout , Natural Killer T-Cells/immunology , Ovalbumin/immunology , Respiratory Hypersensitivity/pathology , Respiratory Hypersensitivity/therapy , Spleen/immunology
4.
Mucosal Immunol ; 5(6): 691-701, 2012 Nov.
Article in English | MEDLINE | ID: mdl-22718263

ABSTRACT

In a biphasic, ovalbumin (OVA)-induced murine asthma model where allergic airway disease is followed by resolution and the development of local inhalational tolerance (LIT), transforming growth factor (TGF)-ß-expressing CD5(+) B cells were selectively expanded locally in hilar lymph nodes (HLN) of LIT mice. LIT HLN CD5(+) B cells, but not LIT HLN CD5(-) B cells, induced expression of Foxp3 in CD4(+)CD25(-) T cells in vitro. These CD5(+) regulatory B cells (Breg) and CD4(+)Foxp3(+) T cells demonstrated similar increases in expression of chemokine receptors (CXCR4 and CXCR5) and co-localized in HLN B cell zones of LIT mice. The adoptive transfer of LIT HLN CD5(+) B cells, but not LIT HLN CD5(-) B cells, increased the number of CD4(+)Foxp3(+) T cells in the lung and inhibited airway eosinophilia in this OVA model. Thus, Breg in HLNs of LIT mice reside in a CD5(+) TGF-ß-producing subpopulation and co-localize with CD4(+)Foxp3(+) T cells.


Subject(s)
Asthma/immunology , B-Lymphocytes, Regulatory/immunology , Eosinophilia/immunology , Immune Tolerance , Lung/immunology , Lymph Nodes/immunology , T-Lymphocytes, Regulatory/immunology , Adoptive Transfer , Animals , Asthma/chemically induced , Asthma/metabolism , Asthma/pathology , B-Lymphocytes, Regulatory/cytology , B-Lymphocytes, Regulatory/metabolism , B-Lymphocytes, Regulatory/transplantation , CD4 Antigens/genetics , CD4 Antigens/immunology , CD5 Antigens/genetics , CD5 Antigens/immunology , Cell Proliferation , Disease Models, Animal , Eosinophilia/chemically induced , Eosinophilia/metabolism , Eosinophilia/pathology , Female , Forkhead Transcription Factors/genetics , Forkhead Transcription Factors/immunology , Gene Expression , Lung/metabolism , Lung/pathology , Lymph Nodes/cytology , Lymph Nodes/metabolism , Lymphocyte Count , Mice , Ovalbumin , Receptors, CXCR4/genetics , Receptors, CXCR4/immunology , Receptors, CXCR5/genetics , Receptors, CXCR5/immunology , T-Lymphocytes, Regulatory/cytology , T-Lymphocytes, Regulatory/metabolism , Transforming Growth Factor beta/genetics , Transforming Growth Factor beta/immunology
5.
Biochim Biophys Acta ; 1762(5): 499-501, 2006 May.
Article in English | MEDLINE | ID: mdl-16616459

ABSTRACT

Ovalbumin (OVA)-sensitized wildtype (WT) and endotoxin-resistant (ER) mice developed similar degrees of airways eosinophilia and serum OVA-specific IgE levels after acute aerosolized OVA challenge. WT mice demonstrated methacholine hyperreactivity, whereas ER mice showed no change in responsiveness. With chronic aerosolized OVA challenge, both WT and ER mice developed local tolerance, with resolution of airway eosinophilia but persistence of anti-OVA IgE in serum. Thus, the development of local tolerance with chronic aerosol exposure to OVA is independent of any potential effects of endotoxin in the OVA aerosol solution.


Subject(s)
Antigens/administration & dosage , Antigens/immunology , Asthma/immunology , Disease Models, Animal , Immune Tolerance/immunology , Administration, Inhalation , Animals , Asthma/blood , Endotoxins/immunology , Immunoglobulin E/blood , Mice , Mice, Inbred C57BL , Ovalbumin/immunology
6.
Lung ; 176(6): 393-401, 1998.
Article in English | MEDLINE | ID: mdl-9780297

ABSTRACT

Tannin, isolated from cotton bracts and implicated in the pathogenesis of byssinosis, inhibits isoproterenol and forskolin-stimulated cAMP release from airway cells in part by decreasing cell surface beta-adrenergic receptor number and uncoupling the beta-adrenergic receptor from its stimulatory G-protein (Gs) and in part by inhibiting adenylyl cyclase activity. We have hypothesized that cotton tannin, because of its long polymer length, interacts with the hydrophobic binding pocket of the beta-adrenergic receptor and alters beta-adrenergic receptor binding and Gs coupling. In these studies, tannins of three different polymer lengths and molecular masses were isolated from cotton bracts using sequential Amicon ultrafiltration [molecular mass > 10, 000 (YM10 retentate), 1,000-10,000 (YM10 filtrate), and 1,000-5,000 Da (YM2 retentate)]. The YM10 retentate (25 microg/ml) decreased chloride secretion (Jnet = 1.11 +/- 0.28 (control) to 0.59 +/- 0.18 microEq/cm2.h, p < 0.05, n = 6), decreased cell surface beta-adrenergic receptor number (18.0 +/- 1.8 (control) to 10.6 +/- 0.9 fmol/mg protein, p < 0.02, n = 4), and inhibited forskolin-stimulated cAMP release (5,254 +/- 1,290 (control) to 2, 968 +/- 620 pmol/mg protein, p < 0.01, n = 8). In contrast, neither the YM10 filtrate nor the YM2 retentate had any effect on net chloride secretion, beta-adrenergic cell surface receptor number, or forskolin-stimulated cAMP release. We conclude that polymer length is essential for the effect of tannin on the beta-adrenergic receptor and on adenylyl cyclase.


Subject(s)
Biopolymers/physiology , Byssinosis/etiology , Hydrolyzable Tannins/pharmacology , Receptors, Adrenergic, beta/drug effects , Trachea/drug effects , Animals , Cattle , Chlorides/analysis , Chlorides/metabolism , Colforsin/pharmacology , Culture Techniques , Cyclic AMP/analysis , Cyclic AMP/metabolism , Gossypium/chemistry , Molecular Weight , Receptors, Adrenergic, beta/analysis , Receptors, Adrenergic, beta/metabolism , Trachea/cytology
7.
Am J Physiol ; 274(2): L252-7, 1998 02.
Article in English | MEDLINE | ID: mdl-9486210

ABSTRACT

Tannin, isolated from cotton bracts, inhibits chloride secretion in airway epithelium. In bovine tracheal epithelial cells, tannin (25 micrograms/ml) blunted isoproterenol (Iso)-stimulated adenosine 3',5'-cyclic monophosphate (cAMP) accumulation. Inhibition was time and dose dependent, with 52 +/- 5% (mean +/- SE, n = 6) inhibition at 60 min and 82 +/- 9% (n = 3) inhibition at 8 h. Inhibition was reversible starting at 4 h. Low-molecular-mass tannin (1,000-5,000 Da) had no effect on Iso-stimulated cAMP accumulation, whereas N-acetylcysteine, which interacts with cysteine residues, blocked the effects of tannin on Iso-stimulated cAMP accumulation. Tannin exposure (25 micrograms/ml for 30 min) had no effect on the dissociation constant (Kd) for [3H]dihydroalprenolol (DHA) (0.41 +/- 0.03 nM, n = 3) but decreased maximal binding from 252 +/- 32 to 162 +/- 36 fmol/mg protein. Using single-point analysis and [3H]CGP-12177, we determined that tannin (25 micrograms/ml for 4 h) decreased surface beta-adrenergic receptor density from 26.4 +/- 4.3 (n = 12) to 11.9 +/- 3.0 fmol/mg protein and that the decrease was dose dependent. Agonist binding affinity by Iso displacement of DHA demonstrated a two-site model (Kd values = 27 +/- 9 and 2,700 +/- 600 nM) and a ratio of high- to low-affinity receptors of 1:1. Tannin (25 micrograms/ml) steepened the curve and shifted it to the right, as did Gpp(NH)p. Gpp(NH)p had no further effect on the shape or position of the displacement curve in the presence of tannin. In contrast, when polymer length was decreased by oxidation, tannin had no effect on the DHA displacement curve. These data demonstrate that tannin reversibly desensitizes bovine tracheal epithelial cells to Iso, decreases beta-adrenergic receptor density, and uncouples the receptor from its stimulatory G protein. These data also suggest that the polymer length of tannin and its interaction with cysteine residues are important for these effects. These studies provide additional evidence for the role of tannin in the occupational lung disease byssinosis.


Subject(s)
Cyclic AMP/metabolism , Hydrolyzable Tannins/pharmacology , Receptors, Adrenergic, beta/metabolism , Trachea/drug effects , Acetylcysteine/pharmacology , Adrenergic beta-Agonists/pharmacology , Adrenergic beta-Antagonists/pharmacology , Animals , Cattle , Chloride Channels/metabolism , Dihydroalprenolol/pharmacology , Epithelium/drug effects , Epithelium/metabolism , GTP-Binding Proteins/metabolism , Guanylyl Imidodiphosphate/pharmacology , Propanolamines/pharmacology , Trachea/metabolism
8.
Am J Physiol ; 270(4 Pt 1): L613-8, 1996 Apr.
Article in English | MEDLINE | ID: mdl-8928821

ABSTRACT

Condensed tannin, isolated from cotton bracts extract (CBE), increases arachidonic acid (AA) release from rabbit alveolar macrophages and inhibits its subsequent reacylation. We determined whether tannin from CBE had any effect upon AA release in bovine tracheal epithelial cells (BTE). [14C] AA release was measured at timed intervals after addition of various concentrations of tannin to BTE cells grown to confluence in the presence of [14C] AA. Tannin caused a time- and dose-dependent release of AA from airway cells, with a maximum release occurring at 1 min in the presence of 100 micrograms/ml of tannin, and was confirmed by high-pressure liquid chromatography. The pattern of release was similar to that observed with bradykinin (2 x 10(-6) M). AA release by tannin was partially inhibited by indomethacin (10(-5) M) but not by 5,8,11,14-eicosatetraynoic acid (ETYA; 10(-5) M. Both of these drugs were effective in inhibiting bradykinin-induced AA release. In addition, AA release was not inhibited by cycloheximide. Endotoxin at 100 pg/ml and higher also caused a time-dependent release of AA that was not inhibitable by indomethacin or ETYA. Tannin-induced AA release was inhibited by pretreatment with pertussis toxin but not by neomycin, an inhibitor of phospholipase C (PLC). Neither pertussis toxin nor neomycin had any effect upon endotoxin-induced AA release. In other experiments, neither tannin nor endotoxin had any effect on [14C]AA uptake by BTE. These data demonstrate that tannin at low concentrations and endotoxin at high concentrations increase AA release by BTE cells. The AA release by tannin is partially metabolized by the cyclooxygenase pathway. We hypothesize that tannin-induced AA release is not mediated by PLC but may be mediated by other phospholipases, including PLA2.


Subject(s)
Arachidonic Acid/metabolism , Hydrolyzable Tannins/pharmacology , Trachea/metabolism , 5,8,11,14-Eicosatetraynoic Acid/pharmacology , Animals , Arachidonic Acid/antagonists & inhibitors , Cattle , Chromatography, High Pressure Liquid , Drug Combinations , Endotoxins/pharmacology , Epithelial Cells , Epithelium/drug effects , Epithelium/metabolism , Indomethacin/pharmacology , Osmolar Concentration , Pertussis Toxin , Trachea/cytology , Trachea/drug effects , Virulence Factors, Bordetella/pharmacology
9.
Am J Physiol ; 268(5 Pt 1): L851-5, 1995 May.
Article in English | MEDLINE | ID: mdl-7762688

ABSTRACT

Tannin, isolated from cotton bracts extract and implicated in the pathogenesis of byssinosis, inhibits adenosine 3',5'-cyclic monophosphate (cAMP) production and Cl- secretion in bovine airway epithelial cells in part by inhibiting adrenergic receptor binding. The purpose of this study was to determine whether tannin affected other parts of the adrenergic-cAMP signal transduction pathway by examining the effect of tannin on guanosine 5'-triphosphate (GTP)-regulatory pathways (G proteins) and on adenylate cyclase activity. cAMP production in confluent airway epithelial cells was measured in the presence of cholera toxin (100 micrograms/ml), an activator of GS proteins, and forskolin (0.1-1,000 microM), a direct activator of adenylate cyclase. Cholera toxin stimulated cAMP production; this response, however, was inhibited in cells pretreated with 50 micrograms/ml tannin. Forskolin (100 microM) stimulated cAMP production 13-fold above baseline values. Tannin pretreatment inhibited the stimulatory effect of forskolin on cAMP release in a dose-dependent manner with a tannin concentration causing 50% inhibition of 7.5 micrograms/ml. The stimulatory effect of forskolin on cAMP release was completely inhibited in cells pretreated with 50 micrograms/ml tannin. The inhibition was reversible 3 h after removal of tannin from the solution. Tannin also inhibited forskolin-stimulated adenylate cyclase activity in a dose-dependent, noncompetitive manner. We conclude that forskolin and cholera toxin stimulate cAMP production in airway epithelial cells and that tannin inhibits the production of cAMP in airway epithelial cells by a direct effect on adenylate cyclase activity.


Subject(s)
Adenylyl Cyclase Inhibitors , Hydrolyzable Tannins/pharmacology , Trachea/enzymology , Adenylyl Cyclases/metabolism , Animals , Cattle , Cells, Cultured , Cholera Toxin/pharmacology , Colforsin/pharmacology , Cyclic AMP/metabolism , Dose-Response Relationship, Drug , Intracellular Membranes/metabolism , Trachea/cytology
10.
Lung ; 173(5): 307-19, 1995.
Article in English | MEDLINE | ID: mdl-7564489

ABSTRACT

Tannin, a polydisperse polyphenol extracted from cotton bracts (CBE), has been implicated in the pathogenesis of byssinosis, a lung disease of mill workers. CBE tannin inhibits chloride secretion in airway epithelial cells by means of an unknown mechanism(s). Activation of protein kinase C (PKC) by PMA (phorbol 12-myristate 13-acetate) in airway cells increases chloride secretion. The effect of tannin on this PKC pathway was examined, using canine tracheal epithelium mounted in Ussing chambers. PMA addition (10 nM) to the mucosal bath resulted in a 0.36 +/- 0.07 microEq/cm2.h (mean +/- SEM, n = 20) increase in short-circuit current (Isc) and a 0.38 +/- 0.17 microEq/cm2.h increase in net chloride secretion (Jnet). The inactive 4 alpha-phorbol had no effect. Tannin addition to the mucosal bath produced a dose-dependent decrease in Isc and Jnet. In tissues pretreated with 2-50 micrograms/ml tannin, and subsequently stimulated with PMA, tannin inhibited PMA stimulation of chloride secretion beginning at a tannin concentration of 10 micrograms/ml (0.09 +/- 0.05 microEq/cm2.h [n = 10] increase in Isc and 0.08 +/- 0.03 microEq/cm2.h increase in Jnet with PMA after tannin pretreatment). At 50 micrograms/ml tannin, the stimulatory effect of PMA was completely abolished. The known PKC inhibitor, H-7 (20 microM), inhibited PMA stimulation, while chelerythrine (2 microM) had not effect on PMA-stimulated Isc and Jnet, and calphostin C was toxic to the airway epithelium. In membrane fragments, 2.5 micrograms/ml tannin inhibited the rate of histone III phosphorylation by PMA from 32.1 +/- 4.4 nmol/mg protein per min to 20.1 +/- 2.7 nmol/mg protein per min (n = 7). In bovine airway cells, tannin pretreatment (2.5 micrograms/ml) decreased the cytosolic activity of PKC but had no effect on PKC translocation to the membrane. We conclude that tannin inhibits chloride secretion in airway epithelial cells in part by inhibiting PKC.


Subject(s)
Astringents/pharmacology , Chloride Channels/drug effects , Hydrolyzable Tannins/pharmacology , Protein Kinase C/antagonists & inhibitors , Trachea/cytology , Animals , Cattle , Cells, Cultured , Dogs , Dose-Response Relationship, Drug , Epithelial Cells , Membrane Potentials/drug effects
11.
Am J Respir Cell Mol Biol ; 10(1): 106-12, 1994 Jan.
Article in English | MEDLINE | ID: mdl-8292375

ABSTRACT

Tannin, isolated from aqueous extracts of cotton bracts, inhibits chloride secretion in airway epithelial cells. The effect of tannin on the epinephrine- and bradykinin-stimulated rise in intracellular free calcium and cyclic adenosine monophosphate (cAMP) was examined using bovine tracheal epithelial cells in suspension and culture. Basal intracellular calcium levels were 33 +/- 11 nM (mean +/- SEM, n = 54) and increased 13- to 15-fold after addition of epinephrine (10(-6) M) or bradykinin (2 x 10(-6) M). Tannin pretreatment blunted the subsequent response to epinephrine beginning at a tannin concentration of 10 micrograms/ml. Pretreatment with 100 micrograms/ml tannin completely inhibited the rise in intracellular free calcium in response to epinephrine but had no effect on the calcium response to bradykinin. In the absence of tannin, both bradykinin and epinephrine increased intracellular levels of cAMP. At a tannin concentration of 10 micrograms/ml, tannin inhibited the rise in intracellular cAMP in cells stimulated with either epinephrine or bradykinin but had no effect on bradykinin-stimulated prostaglandin E2 release. Tannin alone (10 micrograms/ml) increased prostaglandin E2 release. In other studies, tannin inhibited epinephrine binding to airway epithelial cells in a dose-dependent manner. R(o) decreased from 948 +/- 69 fmol/mg protein under control conditions (n = 4) to 587 +/- 131 fmol/mg protein in the presence of 25 micrograms/ml tannin (n = 3). Tannin had no effect upon the Kd for epinephrine binding (132 +/- 12 pM). Tannin had no effect on bradykinin binding to airway epithelial cells.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Calcium/metabolism , Cyclic AMP/metabolism , Hydrolyzable Tannins/pharmacology , Trachea/drug effects , Trachea/metabolism , Animals , Bradykinin/antagonists & inhibitors , Bradykinin/metabolism , Cattle , Cells, Cultured , Dinoprostone/metabolism , Epinephrine/antagonists & inhibitors , Epinephrine/metabolism , Epithelial Cells , Trachea/cytology
12.
Membr Biochem ; 10(2): 107-18, 1993.
Article in English | MEDLINE | ID: mdl-8361390

ABSTRACT

Duramycin increases short-circuit current (Isc) and net Cl- secretion in tracheal epithelium. We measured the intracellular free calcium ([Ca2+]i) response to duramycin using Indo-1 and bovine and canine tracheal cell suspensions, and the effect of an intracellular calcium chelator, BAPTA, and the protein kinase C inhibitor, staurosporine, on the Isc and [Ca2+]i response to duramycin. [Ca2+]i increased in a dose-dependent manner from basal levels of 34 +/- 5 to 949 +/- 136 nM at 5 x 10(-6) M duramycin. Both BAPTA (50 microM) and staurosporine (5-50 nM) pretreatment blunted the increase in Isc and net Cl- secretion produced by duramycin. BAPTA also blunted the rise in [Ca2+]i produced by duramycin (5 x 10(-6) M) in the presence of extracellular calcium (499 +/- 122 nM). In the absence of extracellular calcium, the duramycin-induced (5 x 10(-6) M) rise in [Ca2+]i was blunted from 949 +/- 136 nM (stimulation in the presence of Ca2+) to 621 +/- 122 nM, and was further decreased in the presence of BAPTA to 197 +/- 42 nM. In contrast, staurosporine (50 nM) pretreatment had no effect on the rise in [Ca2+]i produced by duramycin (basal 90 +/- 27 to 861 +/- 110 nM at 5 x 10(-6) M). Duramycin had no effect on [Ca2+]i in human neutrophils. These data demonstrate that duramycin releases calcium from intracellular stores and stimulates the influx of calcium in airway epithelial cells. These data also demonstrate that, in the presence of protein kinase C pathway blockade, an increase in intracellular free calcium is not sufficient for chloride secretion; thus, duramycin-stimulated chloride secretion may depend upon protein kinase C.


Subject(s)
Anti-Bacterial Agents/pharmacology , Calcium/metabolism , Trachea/drug effects , Trachea/metabolism , Alkaloids/pharmacology , Animals , Bacteriocins , Biological Transport , Cattle , Chlorides/metabolism , Dogs , Egtazic Acid/analogs & derivatives , Egtazic Acid/pharmacology , Fluorescent Dyes , In Vitro Techniques , Indoles , Intracellular Fluid/metabolism , Peptides/pharmacology , Staurosporine , Stimulation, Chemical
13.
Am J Physiol ; 262(5 Pt 1): L555-9, 1992 May.
Article in English | MEDLINE | ID: mdl-1317115

ABSTRACT

p-Aminohippurate (PAH) transport in canine tracheal epithelium occurs by a HCO3- -PAH exchange process that is located on the luminal membrane and is inhibited by stilbene derivatives. The effects of increasing concentrations of other organic anions, including probenecid (10-250 microM), dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP; 10-1,000 microM), phenol red (10-250 microM), and urate (25-500 microM), and the organic cation tetraethylammonium bromide (TEA; 250 microM) on PAH transport were examined in canine tracheal epithelium mounted in Ussing chambers. Neither phenol red, urate, nor TEA had any effect on electrophysiological properties or unidirectional or net PAH fluxes. In contrast, beginning at 10 microM, both probenecid and cAMP produced significant decreases in unidirectional and net PAH absorption without change in unidirectional PAH secretion. The initial change in net PAH absorption occurred in the absence of any change in electrophysiological properties. Higher concentrations of both probenecid and cAMP produced further decreases in net PAH absorption and significant changes in electrophysiological properties. Probenecid and cAMP increased the apparent Michaelis constant for PAH absorption without affecting maximum transport rate. The inhibitory constant for probenecid was 1.01 +/- 0.06 x 10(-4) M (mean +/- SE) and for cAMP was 5.18 +/- 0.20 x 10(-4) M. We conclude that PAH transport in canine tracheal epithelium demonstrates competitive inhibition by other organic anions and substrate specificity. We also conclude that the affinity of the exchange transport system is higher for probenecid than for PAH and cAMP.


Subject(s)
Trachea/metabolism , p-Aminohippuric Acid/pharmacokinetics , Animals , Biological Transport , Cyclic AMP/pharmacology , Dogs , Electrophysiology , Epithelium/metabolism , Epithelium/physiology , Phenolsulfonphthalein/pharmacology , Probenecid/pharmacology , Trachea/physiology , Uric Acid/pharmacology , p-Aminohippuric Acid/antagonists & inhibitors
14.
Exp Lung Res ; 18(3): 409-20, 1992.
Article in English | MEDLINE | ID: mdl-1628570

ABSTRACT

Eprazinone therapy improves pulmonary function and arterial pO2 in patients with chronic bronchitis; however, the mechanism of action is unknown. The purpose of this study was to determine if eprazinone alters either lung surfactant levels in bronchoalveolar lavage fluid (BAL) of normal rats, or ion transport across canine tracheal epithelium mounted in Ussing chambers. In the surfactant studies, normal rats were force fed three doses (50, 100, and 200 mg/kg) of eprazinone for 4 days. Eprazinone at a dose of 200 mg/kg significantly increased total and individual (with the exception of phosphatidylinositol) phospholipid levels and decreased total neutral lipids. Lower doses of eprazinone significantly decreased neutral lipid levels without affecting the phospholipids. There was no change in BAL levels of protein or cells and no abnormal histology. In airway epithelial studies, mucosal addition of eprazinone produced a dose-dependent partially reversible decrease in short-circuit current (Isc). The decrease in Isc at lower eprazinone concentrations was accounted for entirely by a decrease in net chloride secretion while at higher concentrations both sodium and chloride transport were affected. Submucosal eprazinone had no affect on ion transport. These studies suggest that eprazinone influences both BAL lipid levels and ion transport, either of which could lead to a beneficial therapeutic effect.


Subject(s)
Antitussive Agents/pharmacology , Bronchoalveolar Lavage Fluid/metabolism , Lipid Metabolism , Propiophenones/pharmacology , Respiratory Transport/drug effects , Trachea/metabolism , Absorption/drug effects , Animals , Chlorides/metabolism , Epithelium/drug effects , Epithelium/metabolism , Lung/drug effects , Lung/metabolism , Lung/pathology , Male , Pulmonary Surfactants/drug effects , Rats , Rats, Inbred F344 , Sodium/metabolism
15.
Am J Physiol ; 259(3 Pt 1): C450-4, 1990 Sep.
Article in English | MEDLINE | ID: mdl-2169195

ABSTRACT

The effect of duramycin, a polypeptide antibiotic, on Cl- transport in canine tracheal epithelium mounted in Ussing chambers was studied. Over a narrow concentration range, duramycin increased short-circuit current (Isc) and net Cl- secretion and had no effect on mannitol flux when added to the mucosal bathing solution. The maximum increase in Isc was observed at a duramycin concentration of 2 X 10(-6) M and was associated with an increase in both unidirectional Cl- fluxes. Higher duramycin concentrations produced a decrease in Isc. Submucosal addition of duramycin had no effect on Isc except at high concentrations. Pretreatment of tissues with mucosal amiloride (10(-4) M) to reduce basal Na+ transport had no effect on the subsequent response to duamycin. In other tissues pretreated with 10(-3) M dibutyryl adenosine 3',5'-cyclic monophosphate (cAMP), duramycin produced a further increase in Isc and net Cl- secretion similar to its effect in nonpretreated tissues. In all instances the increase in Isc was entirely accounted for by an increase in net Cl- secretion. We conclude that duramycin increases Isc and Cl- secretion in airway epithelium. Although the mechanism of activation is not known, these data demonstrate that duramycin increases Cl- secretion by a pathway other than cAMP. An understanding of the mechanism of action of duramycin may further our understanding of Cl- secretion regulation in airway epithelium.


Subject(s)
Anti-Bacterial Agents/pharmacology , Chlorides/metabolism , Trachea/physiology , Animals , Bacteriocins , Cyclic AMP/pharmacology , Dogs , Epithelium/drug effects , Epithelium/physiology , In Vitro Techniques , Kinetics , Membrane Potentials/drug effects , Mucous Membrane/drug effects , Mucous Membrane/physiology , Muscle, Smooth/physiology , Peptides/pharmacology
17.
Dent Clin North Am ; 30(2): 307-25, 1986 Apr.
Article in English | MEDLINE | ID: mdl-3457732

ABSTRACT

The Proplast TMJ condylar implant, the glenoid fossa implant, and the TMJ interpositional implants should be singled out as having provided a new and more predictable mode of TMJ reconstruction. The use of these implants in cases of ankylosis, degenerative joint disease, orthognathic deformities, and traumatic injuries has greatly contributed to the success of these reconstructions. These implants represent the state of the art in TMJ reconstruction and increasing data from longer-term follow-up have shown a rate of success much higher than any previous implant used in the TMJ. TMJ reconstruction requires careful surgical planning, meticulous surgical technique, and intelligent perioperative care. Postoperative physical therapy is considered essential in the management of these patients. Complications related to reankylosis have been virtually eliminated, as no postoperative immobilization is required in most cases. Long-term stability of these implants, due to the ingrowth of tissue into Proplast, appears to be assured. Continued experience with the implants and close follow-up of reconstruction patients is necessary in the future to adequately assess the performance of the newer glenoid fossa and TMJ interpositional implants. Certainly, a new era in TMJ reconstruction has begun, resulting in increased benefits to the patients whom we all serve.


Subject(s)
Joint Prosthesis , Polytetrafluoroethylene , Proplast , Silicone Elastomers , Temporomandibular Joint Disorders/surgery , Adult , Female , Follow-Up Studies , Humans , Joint Prosthesis/methods , Mandibular Condyle/surgery , Patient Care Planning , Physical Therapy Modalities , Postoperative Period , Prosthesis Design , Temporomandibular Joint/surgery , Temporomandibular Joint Disorders/rehabilitation
18.
Oral Surg Oral Med Oral Pathol ; 59(4): 336-9, 1985 Apr.
Article in English | MEDLINE | ID: mdl-3858769

ABSTRACT

Although most odontogenic infections spread locally to adjacent fascial spaces, usually contiguous with the offending odontogenic pathosis, occasionally such an infection can spread rapidly across the midline and appear on the opposite side of the face and neck. Debilitated chronic alcohol abusers who are nutritionally deficient are more likely to develop serious life-threatening infections, either through serious airway involvement as seen in Ludwig's angina or manifest as a gram-negative septicemia with life-threatening shock and even cardiac arrest, than the usual dental patient with cellulitis. Early recognition through a high index of suspicion and vigorous monitoring will pick up the initial manifestation of toxic shock, as noted in this case report.


Subject(s)
Focal Infection, Dental/complications , Periapical Abscess/complications , Shock, Septic/etiology , Aged , Bacteria/isolation & purification , Humans , Male , Periapical Abscess/microbiology , Shock, Septic/microbiology , Suppuration/microbiology
19.
Brain Res ; 299(2): 331-7, 1984 May 14.
Article in English | MEDLINE | ID: mdl-6145495

ABSTRACT

The effects of the hormone testosterone on neurotransmitter synthesis in peripheral sympathetic ganglia were examined in adult male Sprague-Dawley rats. Tyrosine hydroxylase (T-OH), the rate limiting enzyme in catecholamine biosynthesis was examined in the hypogastric (HG), coeliac (CG), and superior cervical ganglion (SCG) subsequent to castration. Initial studies indicated that 2 weeks after surgery, HG T-OH activity fell to approximately 30% of control. In order to more clearly define the pattern of testosterone effects, HG was examined 1, 2 and 4 weeks after surgery. T-OH activity was 67%, 50% and 11% of control at these 3 respective time points, and the observed alteration in T-OH activity appeared to parallel changes in the size of pelvic target organs. Similar hormonal effects did not occur in other peripheral sympathetic ganglia; T-OH activity was unchanged in SCG and CG when examined 1 month after castration. Enzyme activity was restored following replacement therapy with testosterone, whereas the neural metabolite 17-beta estradiol was without effect. The recovery in T-OH activity was associated with partial recovery of target organ size. These studies suggest that hormonal factors regulate neurotransmitter synthesizing enzymes in adult sympathetic neurons and may do so via consequences of alterations in target organs. These observations parallel similar events in the developing nervous system.


Subject(s)
Ganglia, Sympathetic/enzymology , Testosterone/physiology , Tyrosine 3-Monooxygenase/metabolism , Animals , Castration , Estradiol/analogs & derivatives , Estradiol/pharmacology , Male , Rats , Rats, Inbred Strains , Testosterone/analogs & derivatives , Testosterone/pharmacology , Time Factors
20.
Eur J Cancer Clin Oncol ; 20(5): 651-7, 1984 May.
Article in English | MEDLINE | ID: mdl-6234172

ABSTRACT

Rate-limiting steps are defined between methotrexate (MTX) and 5-fluorouracil (FU) or 5-fluorodeoxyuridine (FUdR) and [14C]-formate incorporation into RNA, DNA and protein as a function of the basal rate of dTMP synthesis. When Ehrlich cells are incubated with 0.1 microM FU dR, 1 microM FU and 50 microM MTX for 1-35 min. [3H]-deoxyuridine (UdR) incorporation into DNA is maximally inhibited within 1, 10 and 15 min respectively. The delay in suppression of [3H]-UdR incorporation into MTX-exposed cells compared to cells exposed to FU or FUdR is related to the slow transport of MTX and the increasing free intracellular MTX levels. Influx of MTX is 4 and 10 times slower than FU and FUdR respectively. At 2.5, 5, 10 and 15 min the free intracellular MTX levels (nmol/g dry wt) are 5.8, 7.4, 8.7 and 8.8 respectively. Free intracellular FdUMP is identified 1 min after exposure of cells to FU and FUdR. Antagonism to MTX-suppression of [14C]-formate incorporation into RNA, DNA and protein occurs when cells are simultaneously exposed to MTX and FU or FUdR. However, [14C]-formate incorporation into RNA, DNA and protein is maximally inhibited when Ehrlich tumor cells are incubated with 50 microM MTX for 10 min and then exposed to 1 microM FU for 1 min (a time in which free intracellular MTX is maximal and [3H]-UdR incorporation is maximally suppressed). Hence the sequence and time of administration of FU or FUdR and MTX inhibition of formate incorporation into RNA, DNA and protein is related to the rate of (a) FU, FUdR and MTX transport, (b) FU and FUdR metabolism to FdUMP and (c) generation of maximal free intracellular MTX.


Subject(s)
Carcinoma, Ehrlich Tumor/metabolism , Floxuridine/pharmacology , Fluorouracil/pharmacology , Methotrexate/pharmacology , Animals , Cells, Cultured , DNA, Neoplasm/biosynthesis , Deoxyuridine/metabolism , Drug Interactions , Floxuridine/metabolism , Fluorodeoxyuridylate/metabolism , Fluorouracil/metabolism , Methotrexate/metabolism , Mice , Neoplasm Proteins/biosynthesis , RNA, Neoplasm/biosynthesis , Thymidine Monophosphate/biosynthesis
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