ABSTRACT
BACKGROUND: Premature infants contribute to infant morbidity and mortality especially in low resource settings. Information on tocolytic and/or anti-inflammatory effects of several plant extracts, such as citral, could help prevent preterm birth cases and reduce the number of preterm infants. The aim of this study was to evaluate the in vitro tocolytic and anti-inflammatory effect of citral on myometrial tissues of the human uterus. METHODS: Myometrial samples from uteri obtained after hysterectomy were used in functional tests to evaluate the inhibitory effect of citral on PGF-2α induced contractions. The intracellular cyclic adenosine monophosphate (cAMP) levels generated in response to citral in human myometrial homogenates were measured by ELISAs. Forskolin was used as a positive control. The anti-inflammatory effect of citral was determined through the measurement of two pro-inflammatory cytokines, tumor necrosis factor-α (TNFα) and interleukin (IL)-1ß, and the anti-inflammatory cytokine IL-10, in human myometrial explants stimulated with lipopolysaccharide (LPS). RESULTS: Citral was able to induce a significant inhibition of PGF-2α induced contractions at the highest concentration level (p < .05). Citral caused a concentration-dependent increase in myometrial cAMP levels (p < .05) and a concentration-dependent decrease in LPS-induced TNFα and IL-1ß production, while IL-10 production increased significantly (p < .05). The anti-inflammatory and tocolytic effects induced by citral could be associated with an increase in cAMP levels in human myometrial samples. CONCLUSION: These properties place citral as a potentially safe and effective adjuvant agent in preterm birth cases, an obstetric and gynecological problem that requires urgent attention.
Subject(s)
Myometrium , Premature Birth , Acyclic Monoterpenes , Female , Humans , Infant , Infant, Newborn , Infant, Premature , Pregnancy , Premature Birth/drug therapy , Premature Birth/prevention & controlABSTRACT
Tipo de estudio: Transversal descriptivo.Objetivos: Identificar el agente etiológico por cultivo en sangre y heces a los pacientes con cuadro clínico sospechoso de salmonelosis, y analizar el verdadero valor de la prueba reacción de Widal de la que se ha hecho un uso indiscriminado.Materiales y Métodos: Se estudiaron 59 pacientes que acudieron a Emergencia del hospital de Infectología Dr. José Daniel Rodríguez Maridueña, de ambos sexos, mayores de doce años, entre noviembre 2002 y mayo 2003. Se les realizó hemograma, reacción de Widal, hemocultivo y coprocultivo en el Instituto Nacional de Higiene y Medicina Tropical Leopoldo Izquieta Pérez.Resultados: Se aisló salmonela Typhi en el 3,8% de hemocultivos realizados a pacientes con RW positiva y sospecha de fiebre tifoidea; en el 21,7% de los pacientes que presentaban clínica más leucopenia y en el 25%, con clínica, leucopenia y RW positiva. Se aisló enterobacterias en el 12% de los pacientes con gastroenteritis; 5% salmonela no tifoideas grupo C, 5% E. Coli y, 2% shiguela.Conclusiones: Se confirmó que la RW no es concluyente en el diagnóstico de Fiebre Tifoidea, y que el cuadro clínico de gastroenteritis por salmonela es indiferenciable del ocasionado por otros agentes bacterianos o no.
Type of study: Transversal descriptive.Objectives: Identify the etiologic agent by blood and stool culture and analize the real value of Widal test.Materials and Methods: 59 patients with clinical features of salmonellosis that went to the emergency room of the Infectology Hospital José Daniel Rodriguez Maridueña of both sexs, older than twelve years old were studied between November 2002 and May 2003. Hemogram, Widal test, blood culture and stool culture were done to each one.Results: Typhoid salmonella was isolated in 3,8% of blood cultures of positive Widal test and suspected of having typhoid fever, in the 21.7% of patients with clinical symptoms and leukopenia and in the 25% of patients with clinical symptoms, leukopenia and positive Widal test. Enterobacterias were isolated in 12% of gastroenteritis patients; 5% group C no Typhi Salmonella, 5% E coli, and 2% shiguella.Conclusions: We confirmed that Widal Test is not conclusive in the typhoid fever diagnosis and that the clinical features of salmonella gastroenteritis is indistinguishable from those caused by other bacterial or not bacterial agents.
Subject(s)
Male , Adult , Female , Salmonella Infections , Typhoid Fever , Abdominal Pain , Diarrhea , Salmonella enterica , Salmonella typhiABSTRACT
Las heridas por arma de fuego se asocian con un gran número de órganos lesionados por factores como: tipo de proyectil, distancia, velocidad, trayecto, etc. En el siguiente trabajo se presenta a paciente femenina de 44 años que ingresa al hospital por presentar múltiples heridas por arma de fuego, cursa con hemorragia, equímosis, edema en hemicara izquierda y hemitórax posteroinferior izquierdo. Se le realiza laparotomía exploratoria encontrándose órganos abdominales indemnes y toracotomía en campo pulmonar izquierdo. Siendo hospitalizada para su seguimiento. Hasta el momento no se reportan complicaciones durante el postoperatorio.
