ABSTRACT
The alpha B-crystallin gene (CRYA2) encodes the abundant lens protein alpha B-crystallin. A panel of human/rodent hybrid cell lines, derived from five different parental combinations, was characterized with respect to human chromosomal content and the presence of well-established human chromosome-specific markers. This panel was screened for the presence of CRYA2, using the third exon of the hamster alpha B-crystallin gene as a probe. The patterns of segregation of CRYA2 with individual human chromosomes show the highest degree of concordance between CRYA2 and chromosome 11. Using cell hybrids containing translocated and/or partially deleted human chromosomes, the CRYA2 gene was localized to 11q12-11q23.
Subject(s)
Chromosome Mapping , Chromosomes, Human, Pair 11 , Crystallins/genetics , HumansABSTRACT
Interlineage human-mouse hybrids were constructed by fusion of human acute undifferentiated leukaemia cells with the mouse thymoma cell line BW5147. Some of the hybrids expressed the human differentiation antigens CD4, CD7, CD33, and CD71 (transferrin receptor). Chromosome analysis revealed that the expression of the myeloid antigen CD33 is dependent on the presence of human chromosome 19, which is in agreement with the location of CD33-coding sequences on chromosome 19, as recently reported by Peiper et al. (1987). Furthermore, these hybrids allowed us to confirm the assignment of the CD4 antigen, the CD7 antigen, and the CD71 antigen to human chromosomes 12, 17 and 3, respectively.
Subject(s)
Antigens, CD/genetics , Antigens, Differentiation, Myelomonocytic/genetics , Chromosomes, Human, Pair 19 , Leukemia/genetics , Adult , Animals , Antigens, CD/biosynthesis , Antigens, CD7 , Antigens, Differentiation, Myelomonocytic/biosynthesis , Antigens, Differentiation, T-Lymphocyte/biosynthesis , Antigens, Differentiation, T-Lymphocyte/genetics , CD4 Antigens/biosynthesis , CD4 Antigens/genetics , Chromosome Banding , Humans , Hybrid Cells , Male , Mice , Receptors, Transferrin/biosynthesis , Receptors, Transferrin/genetics , Sialic Acid Binding Ig-like Lectin 3 , Tumor Cells, CulturedABSTRACT
Human probes identifying the cellular homologs of the v-ets gene, Hu-ets-1 and Hu-ets-2, and two panels of rodent-human cell hybrids were used to study specific translocations occurring in acute leukemias. The human ets-1 gene was found to translocate from chromosome 11 to 4 in the t(4;11)(q21;23), a translocation characteristic of a subtype of leukemia that represents the expansion of a myeloid/lymphoid precursor cell. Similarly, the human ets-2 gene was found to translocate from chromosome 21 to chromosome 8 in the t(8;21)(q22;q22), a nonrandom translocation commonly found in patients with acute myeloid leukemia with morphology M2 (AML-M2). Both translocations are associated with expression different from the expression in normal lymphoid cells of ets genes, raising the possibility that these genes play a role in the pathogenesis of these leukemias.
