ABSTRACT
Many plant species containing flavonoids have been widely used in traditional Chinese medicine. Naringin, a well-known flavanone glycoside of citrus fruits, possesses antioxidant, anti-inflammatory, anti-apoptotic, anti-ulcer, anti-osteoporosis, and anti-carcinogenic properties. The aim of the study was to investigate the anti-asthmatic effects of naringin and the possible mechanisms. Asthma model was established by ovalbumin. A total of 50 mice were randomly assigned to five experimental groups: control, model, and dexamethasone (2 mg/kg, orally) and naringin (5 mg/kg, 10 mg/kg, orally). Airway resistance (Raw) were measured, histological studies were evaluated by the hematoxylin and eosin (HE) staining, OVA-specific serum and BALF IgE levels and Th1/Th2 cytokines were evaluated by enzyme-linked immunosorbent assay (ELISA), and Th1/Th2 cells was evaluated by flow cytometry (FCM). T-bet and GABA3 in the lung were evaluated by Western blot. Our study demonstrated that naringin inhibited OVA-induced increases in Raw and eosinophil count; OVA-induced effects on interleukin (IL)-4 and INF-gamma levels were blunted with naringin administration. Histological studies demonstrated that naringin substantially inhibited OVA-induced eosinophilia in lung tissue and airway tissue. Flow cytometry studies demonstrated that naringin substantially inhibited Th2 cells and enhanced Th1 cells. Naringin substantially inhibited GABA3 and increased T-bet. These findings suggest that naringin may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.
Subject(s)
Anti-Asthmatic Agents/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Asthma/drug therapy , Eosinophilia/drug therapy , Flavanones/therapeutic use , Th1-Th2 Balance/drug effects , Animals , Asthma/chemically induced , Bronchoalveolar Lavage Fluid/chemistry , Dexamethasone/therapeutic use , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Eosinophilia/chemically induced , Female , Immunoglobulin E/immunology , Interferon-gamma/blood , Interleukin-4/blood , Lung/pathology , Medicine, Chinese Traditional , Mice , Mice, Inbred BALB C , Ovalbumin , T-Box Domain Proteins/metabolism , Th1 Cells/immunology , Th2 Cells/immunology , gamma-Aminobutyric Acid/metabolismABSTRACT
We have enhanced the quality and efficiency of nursing through the management of the work by means of standard quantized integration. The measures include formulation in the first place of various quantized standards and detailed assessment rules and then the working out of assessment methods. The adoption of these measures in our hospital has led to the improvement of its managerial expertise, the enhancement of its nursing personnels quality and competition awareness, the stimulation of its staffs enthusiasm, and the strengthening of its nursing work in teaching and research management. As a result, nursing in the hospital has become more scientific and efficient.
