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J Vasc Res ; 43(2): 157-65, 2006.
Article in English | MEDLINE | ID: mdl-16407661

ABSTRACT

BACKGROUND: Smooth muscle cell (SMC) proliferation in atherosclerosis is regulated through the interaction of growth factors like platelet-derived growth factor-BB (PDGF-BB) and insulin-like growth factor-1 (IGF-1) and their receptors (R). We hypothesized that serum starvation of SMCs may affect PDGFbeta-R and IGF-1-R expression and, consequently, the effect of their cognate ligands on SMC survival/proliferation. METHODS AND RESULTS: Serum starvation significantly increases PDGFbeta-R but not IGF-1-R mRNA and protein expression in SMCs. PDGF-BB stimulates cell survival but not proliferation in serum-starved SMCs of the synthetic phenotype, whereas SMCs of the contractile phenotype respond to PDGF-BB by a significant increase in proliferation. Immunohistochemical analysis of coronary atherosclerotic lesions reveals PDGFbeta-R expression in SMCs in the lamina fibromuscularis, but not in the media and in healthy parts of the arterial wall. No such differential expression was observed for IGF-1-R. CONCLUSIONS: Differential regulation of PDGFbeta-R and IGF-1-R expression by serum starvation might represent a mechanism for the control of SMC survival/proliferation in atherogenesis and restenosis. The distribution of PDGFbeta-Rs and IGF-1-Rs in atherosclerotic lesions may indicate an effect of serum starvation on SMCs in the arterial wall.


Subject(s)
Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/physiopathology , Receptor, IGF Type 1/biosynthesis , Receptor, Platelet-Derived Growth Factor beta/biosynthesis , Becaplermin , Cell Proliferation , Cells, Cultured , Coronary Artery Disease/pathology , Coronary Artery Disease/physiopathology , Culture Media, Serum-Free , Down-Regulation , Humans , Platelet-Derived Growth Factor/pharmacology , Proto-Oncogene Proteins c-sis , Up-Regulation
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