ABSTRACT
BACKGROUND: Colorectal cancers (CRC) with high level of microsatellite instability (MSI-H) are characterized by lower metastasis propensity and better prognosis than their stable microsatellite (MSS) counterpart. It was hypothesized that the difference in cancer progression might be related to distinct gelatinase-tissue inhibitors of metalloproteinase (TIMPs) balance in MSI-H and MSS sporadic CRC. PATIENTS AND METHODS: Levels of gelatinase-A (MMP-2) and -B (MMP-9), TIMP-1 and -2 and membrane-type matrix metallo-proteinase-1 (MT1-MMP) were compared in tumors and normal mucosa from patients with MSI-H and MSS CRC. RESULTS: Active levels of MMP-2 and -9, normalized to normal mucosa, were lower in MSI-H than MSS CRC. There was a trend for higher levels of TIMP-1 and TIMP-2 within MSI-H tumors compared with MSS tumors (p=0.08 and p=0.15, respectively), while TIMP-2 amounts were significantly higher in adjacent normal tissue (p<0.001) in patients with MSI-H vs. MSS cancers. There was also a trend for lower MT1-MMP activity in MSI-H than in MSS CRC. CONCLUSION: Our data suggest that the distinct invasive and metastatic behaviors of MSI-H and MSS CRC may be related to different patterns of gelatinase secretion and regulation.
Subject(s)
Colorectal Neoplasms/pathology , Metalloproteases/metabolism , Microsatellite Instability , Tissue Inhibitor of Metalloproteinases/metabolism , Aged , Colorectal Neoplasms/genetics , Colorectal Neoplasms/metabolism , Electrophoresis, Polyacrylamide Gel , Female , Humans , Intestinal Mucosa/metabolism , Intestinal Mucosa/pathology , Male , Matrix Metalloproteinase 14/metabolism , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/metabolism , Middle Aged , Tissue Inhibitor of Metalloproteinase-1/metabolism , Tissue Inhibitor of Metalloproteinase-2/metabolismSubject(s)
Antineoplastic Agents/therapeutic use , Flushing/etiology , Gastrointestinal Stromal Tumors/complications , Piperazines/therapeutic use , Pyrimidines/therapeutic use , Rectal Neoplasms/complications , Aged , Benzamides , Flushing/drug therapy , Gastrointestinal Stromal Tumors/diagnosis , Gastrointestinal Stromal Tumors/drug therapy , Humans , Imatinib Mesylate , Male , Rectal Neoplasms/diagnosis , Rectal Neoplasms/drug therapyABSTRACT
With the July 30th 2004 memorandum, for the first time a text is specifically dedicated to the architecture of the autopsy room. This memorandum reaffirms certain technical specifications stated in the May 7th 2001 decree applicable to hospital mortuaries. It supplements or modifies certain elements, particularly liquid waste processing, which will require new arrangements in death chambers and new expenditures for hospital administrations. It includes the principle of precaution and requires a new approach to handling human corpses in the autopsy room.
Subject(s)
Autopsy/methods , Pathology Department, Hospital/standards , Autopsy/standards , Cadaver , France , Hospital Design and Construction , Humans , HygieneABSTRACT
The objectives of this article are to clarify the concepts of violent death, suspicious death and natural death, and to define the situations which need to be reported to the coroner's office, while referring to the recommendations of the National Order of the Doctors and to the European recommendation n degrees R (99) 3 about the harmonisation of the rules in the field of forensic autopsy. In practice, the indications for forensic autopsy are as follows: homicide or suspicion of homicide, suicide or suspicion of suicide, violation of human rights, death in custody or potentially associated actions of police or army, death engaging a possible responsibility, death bringing into play a particular legislation, collective catastrophe, unexpected sudden death, not identified bodies or skeletal remainders. A guiding principle is that an unexpected death remains non natural until forensic proof of the opposite!
Subject(s)
Death Certificates/legislation & jurisprudence , France , Homicide/legislation & jurisprudence , Humans , Suicide/legislation & jurisprudenceABSTRACT
Broncholithiasis is characterized by calcified perihilar and mediastinal lymph nodes eroding into the tracheobronchial tree. We report herein 4 cases of symptomatic broncholithiasis managed by surgical resection in 2 cases and bronchoscopic removal in 2 cases. From our experience and from the literature review, bronchoscopic removal should be considered in cases of uncomplicated and loose broncholithiasis, whereas surgical management should be chosen first in complicated cases such as obstructive pneumonitis, bronchiectasis, massive hemoptysis, and bronchoesophageal fistulas.
Subject(s)
Bronchial Diseases/microbiology , Bronchial Diseases/surgery , Tuberculosis, Pulmonary/complications , Aged , Aspergillosis/diagnosis , Bacterial Infections/diagnosis , Bronchial Diseases/diagnosis , Bronchoscopy , Female , Humans , Male , Middle AgedABSTRACT
Malignant mesenchymomas are rare soft tissue tumors of mesenchymal origin. We report a case of pleural localization with liposarcomatous, leiomyosarcomatous and osteocartilaginous elements. The treatment associated surgical resection, chemotherapy and radiotherapy. Sixteen months after the diagnosis a metastatic retroperitoneal sarcoma with osteosarcomatous differentiation appeared without any sign of thoracic recurrence. A surgical resection was performed, but a rapid retroperitoneal recurrence led to death.
