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Objective To study the effects of Ganoderma lucidum polysaccharide (GLP) on PBMCs and the related immune mechanism.Methods PBMCs from cancer patients and healthy donors were isolated and treated with different doses of Ganoderma lucidum polysaccharides (10 ng/ml,50 ng/ml and 100 ng/ml).DC cell costimulatory molecules (HLA-DR,CD83,and CD11 c),Th1 (CD3 + CD8-IFN-γ+) cells,Th2 (CD3 + CD8-IL-4+) cells and NK (CD3-CD56+) were analyzed by FCM.Furthermore,The CD3+ CD4+ Th ceils were separated by immunomagnetic beads and stimulated with Ganoderma lucidum polysaccharides at different concentrations in culture.After 24 h,the cytokine expression levels of Th1 and Th2were detected by RT-PCR.The expressions of Th1 differentiation-related transcription factor,STAT4,were analyzed by Western blot.Results Ganoderma lucidum polysaccharides can significantly stimulate in vitro Thl cell differentiation (P＜0.01) in a dose depend manner.It correlates with an increased expression of STAT4 and the elevated mRNA expression levels of Th1 cytokineincluding IL-12,IFN-γ and TNF-α (P＜0.01).Conclusion Ganoderma lucidum polysaccharides may promote Th1 differentiation and increase the secretion of Th1 cvtokines through the upregulation of STAT4.
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Objective To study the effects of the Yinlingtong capsules on intracellular triglyceride,fatty acid oxidation levels and CPT-1/ACC-β in HHL-5 cells.Methods After treated HHL-5 cells with different doses Yinlingtong capsules,intracellular triglyceride content and the fatty acid oxidation level were detected by the kits.The CPT-1β and ACC-β mRNA levels were detected by Q-PCR.Results Yinlingtong capsules could reduce triglyceride content (compared with the control group,triglyceride content of0.5,1 and 2 mg/ml dose group were reduced by 9.5％,14.3％ and 19.4％,respectively),enhance the level of fatty acid oxidation by a dose-response relationship(compared with the control group,the level of fatty acid oxidation of 0.5,1and 2 mg/ml dose group were increased 4.3％,6.9％ and 11.2％,respectively),but could not affect the CPT-1βand ACC-β mRNA levels.Conclusion Yinlingtong capsules reduced the accumulation of fat in HHL-5 hepatocytes,and the specific impact mechanism needs to be further explored.
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Objective To study the effects on inhibit tumor growth and enhance immune function of Ganoderma lucidum polysaccharide(GLP)combined with low-dose cisplatin in tumor-bearing mice.Methods 2 × 106 Lewis cells in 0.1 mL PBS were injected subcutaneously into the flanks of healthy female BALB/c nude mice (5～6 weeks old).When the tumor volume reached 100 mm3,mice were randomly assigned to 4 groups,control group (Oral gavage of 0.5 ml PBS and intraperitoneal injection of 0.5 ml),GLP group (gavage once every two days,40 mg/kg GLP in 0.5 ml),cisplatin group (intraperitoneal injection once every two days,2 mg/kg cisplatin in 0.5 ml) and the combination group (such as the above dose of cisplatin and GLP).After the treatment regimen was complete,the mice were sacrificed,and the tumors were removed,photographed,weighed,and prepared for histological analysis.The immune cells in spleen and peripheral blood were analyzed by flow cytometry.Results GLP can effectively increase anti-tumor effect of the small dose cisplatin,improve the survival of tumor-bearing mice,promote Th cells convert to Th1 in peripheral blood,increased the expression of CD 11c + on DC cell surface (GLP group 4.59％ VS the control group 1.06％,the combined group 7.21％ VS the cisplatin group 2.8％).Conclusion GLP can improve the immune function of tumor-bearing mice,had synergistic anti-tumor growth with low dose cisplatin,moreover,reduce renal toxic effects of cisplatin.
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Objective To explore the changes of the total antioxigenic capacity (T-AOC) of the plasma and red blood cells (RBC) in the acute pulmonary edema rats induced by acute formaldehyde inhalation. Methods SD rats were randomly dived into the formaldehyde group and control group, then were exposed to formaldehyde and physical saline vapor respectively in a chamber for 2 hours, the actions and vital signs were observed. After treatment the rats were taken out the chamber, 5 hours later, the T-AOC of the red blood cells and plasma were measured and the body, lungs and heart were weighted, the lung /heart weight index and lungs/body weight index were calculated, the pathological examination was done. Results Compare with the control group, the lung weight, lung/body weight index, lung /heart weight index and the T-AOC of the plasma increased, the T-AOC of RBC and body weight decreased significantly(P
