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1.
Anim Genet ; 52(3): 251-262, 2021 Jun.
Article in English | MEDLINE | ID: mdl-33829515

ABSTRACT

Icelandic Cattle is the only dairy cattle breed native to Iceland. It currently numbers ca. 26 000 breeding females. We used 50k genotypes of over 8000 Icelandic Cattle to estimate genomic and pedigree-based inbreeding and to detect selection signatures using the integrated haplotype score. We used 47 Icelandic bulls genotyped with a 770k SNP chip to estimate LD decay for comparison with other Nordic dairy cattle breeds. We detected ROHs on the autosomes and computed ROH-based autosomal inbreeding coefficients. Average inbreeding coefficients according to pedigree and ROHs were 0.0621 and 0.101. Effective population sizes for the years 2009-2017 according to pedigree, ROHs, genomic relationship matrix, excess of homozygosity and individual increase in inbreeding were 81, 65, 60, 58 and 92 respectively. We identified three regions and six candidate genes that showed a signature of selection according to the integrated haplotype score (P < 0.05) on chromosomes 1, 16 and 23. The LD structure of Icelandic Cattle is shaped by a long period of isolation and a small founder population. The estimate of LD at distances closer than 0.3 Mb is lower in Icelandic Cattle than in Danish Jersey, but is higher than in Danish Holstein and Red Nordic Dairy Cattle breeds. Our findings show that inbreeding rates in Icelandic Cattle currently are sustainable according to FAO guidelines, and our results do not indicate severe historical inbreeding.


Subject(s)
Cattle/genetics , Inbreeding , Selection, Genetic , Animals , Dairying , Female , Genotype , Iceland , Male , Pedigree , Polymorphism, Single Nucleotide
2.
J Anim Sci ; 94(4): 1426-37, 2016 Apr.
Article in English | MEDLINE | ID: mdl-27136002

ABSTRACT

Male calves and culled cows of dairy cattle are used for beef production. However, unlike beef breeds, the genetics of growth performance traits in dairy breeds have not been extensively studied. Here, we performed a genome-wide association study (GWAS) on Holsteins ( = 5,519), Jerseys ( = 1,231), and Red Dairy Cattle ( = 4,410) to identify QTL for growth traits. First, a GWAS was performed within breeds using whole-genome sequence variants. Later, a meta-analysis was performed to combine information across the 3 breeds. We have identified several QTL that have large effects on growth traits in Holsteins and Red Dairy Cattle but with little overlap across breeds. Only 1 QTL located on chromosome 10 was shared between Holsteins and Red Dairy Cattle. The most significant variant (BTA10:59,164,533, rs43636323; -value = 2.8 × 10) in this QTL explained 2.4% of the total additive genetic variance in Red Dairy Cattle. The gene is a strong candidate for the underlying gene of this QTL. In Red Dairy Cattle, a QTL near 25 Mb on chromosome 14 was very significantly associated with growth traits, consistent with the previously reported gene , which affects growth in beef cattle and humans. No QTL for growth performance was statistically significant in Jerseys, possibly due to the low power of detection with the small sample size. The meta-analysis of the 3 breeds increased the power to detect QTL.


Subject(s)
Cattle/growth & development , Genome-Wide Association Study , Polymorphism, Single Nucleotide , Animals , Cattle/genetics , Female , Male , Phenotype , Quantitative Trait Loci
3.
BMC Genet ; 17: 55, 2016 Mar 22.
Article in English | MEDLINE | ID: mdl-27006194

ABSTRACT

BACKGROUND: The Nordic Red Cattle consisting of three different populations from Finland, Sweden and Denmark are under a joint breeding value estimation system. The long history of recording of production and health traits offers a great opportunity to study production traits and identify causal variants behind them. In this study, we used whole genome sequence level data from 4280 progeny tested Nordic Red Cattle bulls to scan the genome for loci affecting milk, fat and protein yields. RESULTS: Using a genome-wise significance threshold, regions on Bos taurus chromosomes 5, 14, 23, 25 and 26 were associated with fat yield. Regions on chromosomes 5, 14, 16, 19, 20 and 25 were associated with milk yield and chromosomes 5, 14 and 25 had regions associated with protein yield. Significantly associated variations were found in 227 genes for fat yield, 72 genes for milk yield and 30 genes for protein yield. Ingenuity Pathway Analysis was used to identify networks connecting these genes displaying significant hits. When compared to previously mapped genomic regions associated with fertility, significantly associated variations were found in 5 genes common for fat yield and fertility, thus linking these two traits via biological networks. CONCLUSION: This is the first time when whole genome sequence data is utilized to study genomic regions affecting milk production in the Nordic Red Cattle population. Sequence level data offers the possibility to study quantitative traits in detail but still cannot unambiguously reveal which of the associated variations is causative. Linkage disequilibrium creates difficulties to pinpoint the causative genes and variations. One solution to overcome these difficulties is the identification of the functional gene networks and pathways to reveal important interacting genes as candidates for the observed effects. This information on target genomic regions may be exploited to improve genomic prediction.


Subject(s)
Cattle/genetics , Milk/metabolism , Animals , Breeding , Chromosomes, Mammalian , DNA-Binding Proteins/genetics , Denmark , Diacylglycerol O-Acyltransferase/genetics , Dietary Fats/analysis , Fertility/genetics , Finland , Genetic Association Studies , Genomics , Genotyping Techniques , Glutathione Transferase/genetics , Growth Hormone/genetics , Lactation , Linkage Disequilibrium , Male , Milk Proteins/analysis , Mitochondrial Membrane Transport Proteins/genetics , Phenotype , Polymorphism, Single Nucleotide , Quantitative Trait Loci , Sequence Analysis, DNA , Sweden , Trans-Activators/genetics , Ubiquitin-Protein Ligases/genetics
4.
J Anim Breed Genet ; 133(3): 207-18, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26486911

ABSTRACT

Decreased calving performance not only directly impacts the economic efficiency of dairy cattle farming but also influences public concern for animal welfare. Previous studies have revealed a QTL on Bos taurus autosome (BTA) 18 that has a large effect on calving traits in Holstein cattle. In this study, fine mapping of this QTL was performed using imputed high-density SNP chip (HD) genotypes followed by imputed next-generation sequencing (NGS) variants. BTA18 was scanned for seven direct calving traits in 6113 bulls with imputed HD genotypes. SNP rs136283363 (BTA18: 57 548 213) was consistently the most significantly associated SNP across all seven traits [e.g. p-value = 2.04 × 10(-59) for birth index (BI)]. To finely map the QTL region and to explore pleiotropic effects, we studied NGS variants within the targeted region (BTA18: 57 321 450-57 625 355) for associations with direct calving traits and with three conformation traits. Significant variants were prioritized, and their biological relevance to the traits was interpreted. Considering their functional relationships with direct calving traits, SIGLEC12, CD33 and CEACAM18 were proposed as candidate genes. In addition, pleiotropic effects of this QTL region on direct calving traits and conformation traits were observed. However, the extent of linkage disequilibrium combined with the lack of complete annotation and potential errors in the Bos taurus genome assembly hampered our efforts to pinpoint the causal mutation.


Subject(s)
Cattle/physiology , Chromosomes, Mammalian , Quantitative Trait Loci , Reproduction , Animals , Cattle/classification , Cattle/genetics , Chromosome Mapping , Genotype , Male
5.
Animal ; 10(6): 1067-75, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26330119

ABSTRACT

Small reference populations limit the accuracy of genomic prediction in numerically small breeds, such like Danish Jersey. The objective of this study was to investigate two approaches to improve genomic prediction by increasing size of reference population in Danish Jersey. The first approach was to include North American Jersey bulls in Danish Jersey reference population. The second was to genotype cows and use them as reference animals. The validation of genomic prediction was carried out on bulls and cows, respectively. In validation on bulls, about 300 Danish bulls (depending on traits) born in 2005 and later were used as validation data, and the reference populations were: (1) about 1050 Danish bulls, (2) about 1050 Danish bulls and about 1150 US bulls. In validation on cows, about 3000 Danish cows from 87 young half-sib families were used as validation data, and the reference populations were: (1) about 1250 Danish bulls, (2) about 1250 Danish bulls and about 1150 US bulls, (3) about 1250 Danish bulls and about 4800 cows, (4) about 1250 Danish bulls, 1150 US bulls and 4800 Danish cows. Genomic best linear unbiased prediction model was used to predict breeding values. De-regressed proofs were used as response variables. In the validation on bulls for eight traits, the joint DK-US bull reference population led to higher reliability of genomic prediction than the DK bull reference population for six traits, but not for fertility and longevity. Averaged over the eight traits, the gain was 3 percentage points. In the validation on cows for six traits (fertility and longevity were not available), the gain from inclusion of US bull in reference population was 6.6 percentage points in average over the six traits, and the gain from inclusion of cows was 8.2 percentage points. However, the gains from cows and US bulls were not accumulative. The total gain of including both US bulls and Danish cows was 10.5 percentage points. The results indicate that sharing reference data and including cows in reference population are efficient approaches to increase reliability of genomic prediction. Therefore, genomic selection is promising for numerically small population.


Subject(s)
Breeding , Cattle/classification , Cattle/genetics , Genomics/methods , Genomics/standards , Animals , Denmark , Female , Fertility/genetics , Genome/genetics , Genotype , Linear Models , Male , Models, Genetic , Phenotype , Reference Standards , Reproducibility of Results , United States
6.
J Dairy Sci ; 98(6): 4107-16, 2015 Jun.
Article in English | MEDLINE | ID: mdl-25892697

ABSTRACT

This study investigated the effect on the reliability of genomic prediction when a small number of significant variants from single marker analysis based on whole genome sequence data were added to the regular 54k single nucleotide polymorphism (SNP) array data. The extra markers were selected with the aim of augmenting the custom low-density Illumina BovineLD SNP chip (San Diego, CA) used in the Nordic countries. The single-marker analysis was done breed-wise on all 16 index traits included in the breeding goals for Nordic Holstein, Danish Jersey, and Nordic Red cattle plus the total merit index itself. Depending on the trait's economic weight, 15, 10, or 5 quantitative trait loci (QTL) were selected per trait per breed and 3 to 5 markers were selected to tag each QTL. After removing duplicate markers (same marker selected for more than one trait or breed) and filtering for high pairwise linkage disequilibrium and assaying performance on the array, a total of 1,623 QTL markers were selected for inclusion on the custom chip. Genomic prediction analyses were performed for Nordic and French Holstein and Nordic Red animals using either a genomic BLUP or a Bayesian variable selection model. When using the genomic BLUP model including the QTL markers in the analysis, reliability was increased by up to 4 percentage points for production traits in Nordic Holstein animals, up to 3 percentage points for Nordic Reds, and up to 5 percentage points for French Holstein. Smaller gains of up to 1 percentage point was observed for mastitis, but only a 0.5 percentage point increase was seen for fertility. When using a Bayesian model accuracies were generally higher with only 54k data compared with the genomic BLUP approach, but increases in reliability were relatively smaller when QTL markers were included. Results from this study indicate that the reliability of genomic prediction can be increased by including markers significant in genome-wide association studies on whole genome sequence data alongside the 54k SNP set.


Subject(s)
Cattle/genetics , Genome-Wide Association Study , Genomics/methods , Polymorphism, Single Nucleotide , Quantitative Trait Loci , Animals , Bayes Theorem , Europe , Male , Models, Genetic , Reproducibility of Results
7.
J Dairy Sci ; 98(5): 3508-13, 2015 May.
Article in English | MEDLINE | ID: mdl-25771051

ABSTRACT

The effect on prediction accuracy for Jersey genomic evaluations of Danish and US bulls from using a larger reference population was assessed. Each country contributed genotypes from 1,157 Jersey bulls to the reference population of the other. Data were separated into reference (US only, Danish only, and combined US-Danish) and validation (US only and Danish only) populations. Depending on trait (milk, fat, and protein yields and component percentages; productive life; somatic cell score; daughter pregnancy rate; 14 conformation traits; and net merit), the US reference population included 2,720 to 4,772 bulls and cows with traditional evaluations as of August 2009; the Danish reference population included 635 to 996 bulls. The US validation population included 442 to 712 bulls that gained a traditional evaluation between August 2009 and December 2013; the Danish validation population included 105 to 196 bulls with multitrait across-country evaluations on the US scale by December 2013. Genomic predicted transmitting abilities (GPTA) were calculated on the US scale using a selection index that combined direct genomic predictions with either traditional predicted transmitting ability for the reference population or traditional parent averages (PA) for the validation population and a traditional evaluation based only on genotyped animals. Reliability for GPTA was estimated from the reference population and August 2009 traditional PA and PA reliability. For prediction of December 2013 deregressed daughter deviations on the US scale, mean August 2009 GPTA reliability for Danish validation bulls was 0.10 higher when based on the combined US-Danish reference population than when the reference population included only Danish bulls; for US validation bulls, mean reliability increased by 0.02 when Danish bulls were added to the US reference population. Exchanging genotype data to increase the size of the reference population is an efficient approach to increasing the accuracy of genomic prediction when the reference population is small.


Subject(s)
Cattle/genetics , Genomics , Animals , Cattle/classification , Denmark , Female , Genotype , Male , Phenotype , Reproducibility of Results , United States
8.
J Dairy Sci ; 97(11): 7258-75, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25151887

ABSTRACT

Mastitis is a mammary disease that frequently affects dairy cattle. Despite considerable research on the development of effective prevention and treatment strategies, mastitis continues to be a significant issue in bovine veterinary medicine. To identify major genes that affect mastitis in dairy cattle, 6 chromosomal regions on Bos taurus autosome (BTA) 6, 13, 16, 19, and 20 were selected from a genome scan for 9 mastitis phenotypes using imputed high-density single nucleotide polymorphism arrays. Association analyses using sequence-level variants for the 6 targeted regions were carried out to map causal variants using whole-genome sequence data from 3 breeds. The quantitative trait loci (QTL) discovery population comprised 4,992 progeny-tested Holstein bulls, and QTL were confirmed in 4,442 Nordic Red and 1,126 Jersey cattle. The targeted regions were imputed to the sequence level. The highest association signal for clinical mastitis was observed on BTA 6 at 88.97 Mb in Holstein cattle and was confirmed in Nordic Red cattle. The peak association region on BTA 6 contained 2 genes: vitamin D-binding protein precursor (GC) and neuropeptide FF receptor 2 (NPFFR2), which, based on known biological functions, are good candidates for affecting mastitis. However, strong linkage disequilibrium in this region prevented conclusive determination of the causal gene. A different QTL on BTA 6 located at 88.32 Mb in Holstein cattle affected mastitis. In addition, QTL on BTA 13 and 19 were confirmed to segregate in Nordic Red cattle and QTL on BTA 16 and 20 were confirmed in Jersey cattle. Although several candidate genes were identified in these targeted regions, it was not possible to identify a gene or polymorphism as the causal factor for any of these regions.


Subject(s)
Genetic Predisposition to Disease , Genome-Wide Association Study , Mastitis, Bovine/genetics , Polymorphism, Single Nucleotide , Animals , Cattle , Female , Linkage Disequilibrium , Male , Quantitative Trait Loci
9.
J Dairy Sci ; 97(9): 5822-32, 2014 Sep.
Article in English | MEDLINE | ID: mdl-24996280

ABSTRACT

Small dairy breeds are challenged by low reliabilities of genomic prediction. Therefore, we evaluated the effect of including cows in the reference population for small dairy cattle populations with a limited number of sires in the reference population. Using detailed simulations, 2 types of scenarios for maintaining and updating the reference population over a period of 15yr were investigated: a turbo scheme exclusively using genotyped young bulls and a hybrid scheme with mixed use of genotyped young bulls and progeny-tested bulls. Two types of modifications were investigated: (1) number of progeny-tested bulls per year was tested at 6 levels: 15, 40, 60, 100, 250, and 500; and (2) each year, 2,000 first-lactation cows were randomly selected from the cow population for genotyping or, alternatively, an additional 2,000 first-lactation cows were randomly selected and typed in the first 2yr. The effects were evaluated in the 2 main breeding schemes. The breeding schemes were chosen to mimic options for the Danish Jersey cattle population. Evaluation criteria were annual monetary genetic gain, rate of inbreeding, reliability of genomic predictions, and variance of response. Inclusion of cows in the reference population increased monetary genetic gain and decreased the rate of inbreeding. The increase in genetic gain was larger for the turbo schemes with shorter generation intervals. The variance of response was generally higher in turbo schemes than in schemes using progeny-tested bulls. However, the risk was reduced by adding cows to the reference population. The annual genetic gain and the reliability of genomic predictions were slightly higher with more cows in the reference population. Inclusion of cows in the reference population is a rapid way to increase reliabilities of genomic predictions and hence increase genetic gain in a small population. An economic evaluation shows that genotyping of cows is a profitable investment.


Subject(s)
Breeding/methods , Cattle/genetics , Dairying/methods , Genomics/methods , Models, Genetic , Selection, Genetic , Animals , Breeding/economics , Cattle/growth & development , Dairying/economics , Denmark , Female , Genotype , Male , Population Density , Reproducibility of Results
10.
J Dairy Sci ; 97(7): 4485-96, 2014 Jul.
Article in English | MEDLINE | ID: mdl-24792791

ABSTRACT

The main aim of this study was to compare accuracies of imputation and genomic predictions based on single and joint reference populations for Norwegian Red (NRF) and a composite breed (DFS) consisting of Danish Red, Finnish Ayrshire, and Swedish Red. The single nucleotide polymorphism (SNP) data for NRF consisted of 2 data sets: one including 25,000 markers (NRF25K) and the other including 50,000 markers (NRF50K). The NRF25K data set had 2,572 bulls, and the NRF50K data set had 1,128 bulls. Four hundred forty-two bulls were genotyped in both data sets (double-genotyped bulls). The DFS data set (DSF50K) included 50,000 markers of 13,472 individuals, of which around 4,700 were progeny-tested bulls. The NRF25K data set was imputed to 50,000 density using the software Beagle. The average error rate for the imputation of NRF25K decreased slightly from 0.023 to 0.021, and the correlation between observed and imputed genotypes changed from 0.935 to 0.936 when comparing the NRF50K reference and the NRF50K-DFS50K joint reference imputations. A genomic BLUP (GBLUP) model and a Bayesian 4-component mixture model were used to predict genomic breeding values for the NRF and DFS bulls based on the single and joint NRF and DFS reference populations. In the multiple population predictions, accuracies of genomic breeding values increased for the 3 production traits (milk, fat, and protein yields) for both NRF and DFS. Accuracies increased by 6 and 1.3 percentage points, on average, for the NRF and DFS bulls, respectively, using the GBLUP model, and by 9.3 and 1.3 percentage points, on average, using the Bayesian 4-component mixture model. However, accuracies for health or reproduction traits did not increase from the multiple population predictions. Among the 3 DFS populations, Swedish Red gained most in accuracies from the multiple population predictions, presumably because Swedish Red has a closer genetic relationship with NRF than Danish Red and Finnish Ayrshire. The Bayesian 4-component mixture model performed better than the GBLUP model for most production traits for both NRF and DFS, whereas no advantage was found for health or reproduction traits. In general, combining NRF and DFS reference populations was useful in genomic predictions for both the NRF and DFS bulls.


Subject(s)
Breeding , Cattle/genetics , Genomics/methods , Animals , Databases, Genetic , Dietary Fats/analysis , Female , Finland , Genetic Markers , Genome , Genotype , Genotyping Techniques , Lactation , Male , Milk/metabolism , Milk Proteins/analysis , Models, Genetic , Norway , Phenotype , Polymorphism, Single Nucleotide , Reproducibility of Results , Reproduction , Sweden
11.
J Dairy Sci ; 97(1): 458-70, 2014.
Article in English | MEDLINE | ID: mdl-24239076

ABSTRACT

The objective of this study was to evaluate a genomic breeding scheme in a small dairy cattle population that was intermediate in terms of using both young bulls (YB) and progeny-tested bulls (PB). This scheme was compared with a conventional progeny testing program without use of genomic information and, as the extreme case, a juvenile scheme with genomic information, where all bulls were used before progeny information was available. The population structure, cost, and breeding plan parameters were chosen to reflect the Danish Jersey cattle population, being representative for a small dairy cattle population. The population consisted of 68,000 registered cows. Annually, 1,500 bull dams were screened to produce the 500 genotyped bull calves from which 60 YB were selected to be progeny tested. Two unfavorably correlated traits were included in the breeding goal, a production trait (h(2)=0.30) and a functional trait (h(2)=0.04). An increase in reliability of 5 percentage points for each trait was used in the default genomic scenario. A deterministic approach was used to model the different breeding programs, where the primary evaluation criterion was annual monetary genetic gain (AMGG). Discounted profit was used as an indicator of the economic outcome. We investigated the effect of varying the following parameters: (1) increase in reliability due to genomic information, (2) number of genotyped bull calves, (3) proportion of bull dam sires that are young bulls, and (4) proportion of cow sires that are young bulls. The genomic breeding scheme was both genetically and economically superior to the conventional breeding scheme, even in a small dairy cattle population where genomic information causes a relatively low increase in reliability of breeding values. Assuming low reliabilities of genomic predictions, the optimal breeding scheme according to AMGG was characterized by mixed use of YB and PB as bull sires. Exclusive use of YB for production cows increased AMGG up to 3 percentage points. The results from this study supported our hypothesis that strong interaction effects exist. The strongest interaction effects were obtained between increased reliabilities of genomic estimated breeding values and more intensive use of YB. The juvenile scheme was genetically inferior when the increase in reliability was low (5 percentage points), but became genetically superior at higher reliabilities of genomic estimated breeding values. The juvenile scheme was always superior according to discounted profit because of the shorter generation interval and minimizing costs for housing and feeding waiting bulls.


Subject(s)
Cattle/genetics , Genomics/methods , Selection, Genetic , Animals , Breeding , Dairying , Female , Genome , Genotype , Male , Models, Genetic , Phenotype , Reproducibility of Results
12.
Anim Genet ; 45(1): 105-10, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24303917

ABSTRACT

Mapping of QTL affecting fur quality traits (guard hair length, guard hair thickness, density of wool, surface of the fur and quality) and skin length was performed in a three-generation mink population (F2 design). In the parental generation, Nordic Brown mink were crossed reciprocally with American Black short nap mink. In all, 1082 mink encompassing three generations were used for the analyses. The mink were genotyped for 104 microsatellites covering all 14 autosomes. The QTL analyses were performed by least-square regression implemented in gridqtl software. Genetic and phenotypic correlations and heritabilities were estimated using the average information-restricted maximum-likelihood method. Evidence was found for QTL affecting fur quality traits on nine autosomes. QTL were detected for guard hair thickness on chromosomes 1, 2, 3, 6 and 13; for guard hair length on chromosomes 2, 3 and 6; for wool density on chromosomes 6 and 13; for surface on chromosomes 7, 12 and 13; for quality on chromosomes 6, 7, 11 and 13; and for skin length on chromosomes 7 and 9. Proximity of locations of QTL for guard hair length, guard hair thickness and for wool density and quality suggests that some of the traits are in part under the influence of the same genes. Traits under the influence of QTL at close or identical positions also were traits that were strongly genotypically correlated. Based on the results of correlation analyses, the most important single traits influencing the quality were found to be density of wool, guard hair thickness and appearance of the surface.


Subject(s)
Hair , Mink/genetics , Quantitative Trait Loci , Animals , Chromosome Mapping , Genetic Linkage , Genotype , Microsatellite Repeats , Phenotype
13.
J Anim Sci ; 91(7): 3105-12, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23658363

ABSTRACT

The main purpose of this study was to evaluate whether the population structure in Danish Jersey (DJ) known from the history of the breed also is reflected in its genomic structure. This is done by comparing the linkage disequilibrium and persistence of phase for subgroups of Jersey animals with high proportions of Danish (DNK) or United States (USJ) origin. Furthermore, it is investigated whether a model explicitly incorporating breed origin of animals, inferred either through the known pedigree or from SNP marker data, leads to improved genomic predictions compared with a model ignoring breed origin. The study of the population structure incorporated 1,730 genotyped Jersey animals. In total 39,542 SNP markers were included in the analysis. The 1,079 genotyped bulls with de-regressed proof for udder health were used in the analysis for the predictions of the genomic breeding values. A range of random regressions models that included the breed origin were analyzed and compared with a basic genomic model that assumes a homogeneous breed structure. The main finding in this study is that the importation of germplasm from the USJ population is readily reflected in the genomes of modern DJ animals. First, linkage disequilibrium in the group of admixed DJ animals is lower compared with the groups of the original DNK and USJ animals. Second, persistence of linkage disequilibrium phase is not conserved for longer marker distances between animals with mainly Danish or United States origin. Third, the STRUCTURE analysis could retrieve genomic-based breed proportions in alignment to the pedigree-based breed proportions. However, including this population structure in a random regression prediction model did not clearly improve the reliabilities of the genomic predictions compared with a basic genomic model.


Subject(s)
Breeding/methods , Cattle/genetics , Genome , Animals , Denmark , Female , Linear Models , Linkage Disequilibrium , Male , Models, Genetic , Pedigree , Polymorphism, Single Nucleotide , Population Density
14.
Anim Genet ; 44(6): 620-6, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23647142

ABSTRACT

A genome-wide association study of 2098 progeny-tested Nordic Holstein bulls genotyped for 36 387 SNPs on 29 autosomes was conducted to confirm and fine-map quantitative trait loci (QTL) for mastitis traits identified earlier using linkage analysis with sparse microsatellite markers in the same population. We used linear mixed model analysis where a polygenic genetic effect was fitted as a random effect and single SNPs were successively included as fixed effects in the model. We detected 143 SNP-by-trait significant associations (P < 0.0001) on 20 chromosomes affecting mastitis-related traits. Among them, 21 SNP-by-trait combinations exceeded the genome-wide significant threshold. For 12 chromosomes, both the present association study and the previous linkage study detected QTL, and of these, six were in the same chromosomal locations. Strong associations of SNPs with mastitis traits were observed on bovine autosomes 6, 13, 14 and 20. Possible candidate genes for these QTL were identified. Identification of SNPs in linkage disequilibrium with QTL will enable marker-based selection for mastitis resistance. The candidate genes identified should be further studied to detect candidate polymorphisms underlying these QTL.


Subject(s)
Chromosome Mapping/veterinary , Mastitis, Bovine/genetics , Phenotype , Quantitative Trait Loci/genetics , Animals , Cattle , Female , Genetic Linkage/genetics , Genome-Wide Association Study , Genotype , Linear Models , Mastitis, Bovine/pathology , Polymorphism, Single Nucleotide/genetics
15.
J Anim Breed Genet ; 130(2): 128-35, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23496013

ABSTRACT

Genomic selection is a method to predict breeding values using genome-wide single-nucleotide polymorphism (SNP) markers. High-quality marker data are necessary for genomic selection. The aim of this study was to investigate the effect of marker-editing criteria on the accuracy of genomic predictions in the Nordic Holstein and Jersey populations. Data included 4429 Holstein and 1071 Jersey bulls. In total, 48,222 SNP for Holstein and 44,305 SNP for Jersey were polymorphic. The SNP data were edited based on (i) minor allele frequencies (MAF) with thresholds of no limit, 0.001, 0.01, 0.02, 0.05 and 0.10, (ii) deviations from Hardy-Weinberg proportions (HWP) with thresholds of no limit, chi-squared p-values of 0.001, 0.02, 0.05 and 0.10, and (iii) GenCall (GC) scores with thresholds of 0.15, 0.55, 0.60, 0.65 and 0.70. The marker data sets edited with different criteria were used for genomic prediction of protein yield, fertility and mastitis using a Bayesian variable selection and a GBLUP model. De-regressed EBV were used as response variables. The result showed little difference between prediction accuracies based on marker data sets edited with MAF and deviation from HWP. However, accuracy decreased with more stringent thresholds of GC score. According to the results of this study, it would be appropriate to edit data with restriction of MAF being between 0.01 and 0.02, a p-value of deviation from HWP being 0.05, and keeping all individual SNP genotypes having a GC score over 0.15.


Subject(s)
Cattle/genetics , Genetic Markers , Genomics/methods , Polymorphism, Single Nucleotide , Alleles , Animals , Male
16.
Int J Immunogenet ; 40(2): 131-9, 2013 Apr.
Article in English | MEDLINE | ID: mdl-22672630

ABSTRACT

The single nucleotide polymorphism (SNP) G949T in the mannose-binding lectin ( MBL ) 1 gene has been associated with low MBL-A concentration in serum and detected at different frequencies in various European pig populations. However, the origin of this SNP is not known. Part of the MBL1 gene was sequenced in 12 wild boar/Large White crossbred pigs from the second backcross (BC 2 ) generation in a family material originating from two wild boar x Large White intercrosses. Also, MBL-A serum concentration was measured in the entire BC 2 generation (n = 45). Furthermore, the genotypes of 68 wild boars from Sweden, Austria, the Czech Republic, and Japan were determined in regard to five previously described SNPs in MBL1 . The T allele of G949T was present among the BC 2 animals. MBL-A serum concentration in the BC 2 animals showed a bimodal distribution, with one-third of the animals at levels between 0.7 and 1.6 µg mL(-1) and the remaining pigs at levels around 13 µg mL(-1) . There was a co-variation between the presence of the T allele and low MBL-A concentration in serum. The genotyping of the wild boars revealed differences between populations. The T allele of G949T was not detected in the Austrian and Japanese samples and is thus unlikely to be an original feature of wild boars. In contrast, it was present at high frequency (0.35) among the Swedish wild boars, probably representing a founder effect. Five MBL1 haplotypes were resolved. Only two of these were present among the Japanese wild boars compared to four in each of the European populations. This difference may reflect differences in selection pressure and population history.


Subject(s)
Mannose-Binding Lectin/blood , Mannose-Binding Lectin/genetics , Sus scrofa/genetics , Animals , Austria , Base Sequence , Czech Republic , Gene Frequency , Genotype , Haplotypes , Japan , Polymorphism, Single Nucleotide , Receptors, Pattern Recognition/genetics , Sequence Analysis, DNA/veterinary , Sweden
17.
J Anim Breed Genet ; 129(5): 369-79, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22963358

ABSTRACT

Using a combined multi-breed reference population, this study explored the influence of model specification and the effect of including a polygenic effect on the reliability of genomic breeding values (DGV and GEBV). The combined reference population consisted of 2986 Swedish Red Breed (SRB) and Finnish Ayrshire (FAY) dairy cattle. Bayesian methodology (common prior and mixture models with different prior distribution settings for the marker effects) as well as a best linear unbiased prediction with a genomic relationship matrix [genomic best linear unbiased predictor (GBLUP)] was used in the prediction of DGV. Mixture models including a polygenic effect were used to predict GEBV. In total, five traits with low, high and medium heritability were analysed. For the models using a mixture prior distribution, reliabilities of DGV tended to decrease with an increasing proportion of markers with small effects. The influence of the inclusion of a polygenic effect on the reliability of DGV varied across traits and model specifications. Average correlation between DGV with the Mendelian sampling term, across traits, was highest (R(2) = 0.25) for the GBLUP model and decreased with increasing proportion of markers with large effects. Reliabilities increased when DGV and parent average information were combined in an index. The GBLUP model with the largest gain across traits in the reliability of the index achieved the highest DGV mean reliability. However, the polygenic models showed to be less biased and more consistent in the estimation of DGV regardless of the model specifications compared with the mixture models without the polygenic effect.


Subject(s)
Cattle/genetics , Models, Genetic , Animals , Bayes Theorem , Breeding , Female , Finland , Genomics/methods , Linear Models , Linkage Disequilibrium , Male , Quantitative Trait Loci , Reproducibility of Results , Sweden
18.
J Dairy Sci ; 95(8): 4657-65, 2012 Aug.
Article in English | MEDLINE | ID: mdl-22818480

ABSTRACT

This study investigated genomic prediction using medium-density (∼54,000; 54K) and high-density marker panels (∼777,000; 777K), based on data from Nordic Holstein and Red Dairy Cattle (RDC). The Holstein data comprised 4,539 progeny-tested bulls, and the RDC data 4,403 progeny-tested bulls. The data were divided into reference data and test data using October 1, 2001, as a cut-off date (birth date of the bulls). This resulted in about 25% genotyped bulls in the Holstein test data and 20% in the RDC test data. For each breed, 3 data sets of markers were used to predict breeding values: (1) 54K data set with missing genotypes, (2) 54K data set where missing genotypes were imputed, and (3) imputed high-density (HD) marker data set created by imputing the 54K data to the HD data based on 557 bulls genotyped using a 777K single nucleotide polymorphism chip in Holstein, and 706 bulls in RDC. Based on the 3 marker data sets, direct genomic breeding values (DGV) for protein, fertility, and udder health were predicted using a genomic BLUP model (GBLUP) and a Bayesian mixture model with 2 normal distributions. Reliability of DGV was measured as squared correlations between deregressed proofs (DRP) and DGV corrected for reliability of DRP. Unbiasedness was assessed by regression of DRP on DGV, based on the bulls in the test data sets. Averaged over the 3 traits, reliability of DGV based on the HD markers was 0.5% higher than that based on the 54K data in Holstein, and 1.0% higher than that in RDC. In addition, the HD markers led to an improvement of unbiasedness of DGV. The Bayesian mixture model led to 0.5% higher reliability than the GBLUP model in Holstein, but not in RDC. Imputing missing genotypes in the 54K marker data did not improve genomic predictions for most of the traits.


Subject(s)
Cattle/genetics , Genetic Markers , Genome , Models, Genetic , Polymorphism, Single Nucleotide , Selection, Genetic , Animals , Bayes Theorem , Female , Genotype , Male , Quantitative Trait, Heritable , Reproducibility of Results
19.
Animal ; 6(5): 789-96, 2012 May.
Article in English | MEDLINE | ID: mdl-22558926

ABSTRACT

In order to optimize the use of genomic selection in breeding plans, it is essential to have reliable estimates of the genomic breeding values. This study investigated reliabilities of direct genomic values (DGVs) in the Jersey population estimated by three different methods. The validation methods were (i) fivefold cross-validation and (ii) validation on the most recent 3 years of bulls. The reliability of DGV was assessed using squared correlations between DGV and deregressed proofs (DRPs). In the recent 3-year validation model, estimated reliabilities were also used to assess the reliabilities of DGV. The data set consisted of 1003 Danish Jersey bulls with conventional estimated breeding values (EBVs) for 14 different traits included in the Nordic selection index. The bulls were genotyped for Single-nucleotide polymorphism (SNP) markers using the Illumina 54 K chip. A Bayesian method was used to estimate the SNP marker effects. The corrected squared correlations between DGV and DRP were on average across all traits 0.04 higher than the squared correlation between DRP and the pedigree index. This shows that there is a gain in accuracy due to incorporation of marker information compared with parent index pre-selection only. Averaged across traits, the estimates of reliability of DGVs ranged from 0.20 for validation on the most recent 3 years of bulls and up to 0.42 for expected reliabilities. Reliabilities from the cross-validation were on average 0.24. For the individual traits, the reliability varied from 0.12 (direct birth) to 0.39 (milk). Bulls whose sires were included in the reference group had an average reliability of 0.25, whereas the bulls whose sires were not included in the reference group had an average reliability that was 0.05 lower.


Subject(s)
Breeding/methods , Cattle/genetics , Models, Genetic , Phenotype , Selection, Genetic , Animals , Bayes Theorem , Breeding/statistics & numerical data , Crosses, Genetic , Female , Genetic Markers , Genotype , Male , Pedigree , Polymorphism, Single Nucleotide/genetics
20.
J Dairy Sci ; 94(9): 4700-7, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21854944

ABSTRACT

This study investigated the possibility of increasing the reliability of direct genomic values (DGV) by combining reference populations. The data were from 3,735 bulls from Danish, Swedish, and Finnish Red dairy cattle populations. Single nucleotide polymorphism markers were fitted as random variables in a Bayesian model, using published estimated breeding values as response variables. In total, 17 index traits were analyzed. Reliabilities were estimated using a 5-fold cross validation, and calculated as the within-year squared correlation between estimated breeding values and DGV. Marker effects were estimated using reference populations from individual countries, as well as using a combined reference population from all 3 countries. Single-country reference populations gave mean reliabilities across 17 traits of 0.19 to 0.23, whereas the combined reference gave mean reliabilities of 0.26 for all populations. Using marker effects from 1 population to predict the other 2 gave a loss in mean reliability of 0.14 to 0.21 when predicting Swedish or Finnish animals with Danish marker effects, or vice versa. Using Swedish or Finnish marker effects to predict each other only showed a loss in mean reliability of 0.03 to 0.05. A combined Swedish-Finnish reference population led to an average reliability as high as that from the 3-country reference population, but somewhat different for individual traits. The results from this study show that it is possible to increase the reliability of DGV by combining reference populations from related populations.


Subject(s)
Breeding/methods , Cattle/genetics , Animals , Bayes Theorem , Dairying/methods , Genetic Markers/genetics , Genomics/methods , Genotype , Male , Polymorphism, Single Nucleotide/genetics , Quantitative Trait, Heritable , Reference Values
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