ABSTRACT
Serum biochemical parameters were studied in 42 healthy wild-caught adult tamarins (S. mystax), males and females, to determine the normal values. Blood samples were drawn repeatedly, and the serum was tested for aspartate aminotransferase, alanine aminotransferase, isocitric dehydrogenase, serum glucose, serum urea, triglyceride, cholesterol, albumin, and total protein. The results indicated that serum chemistry values were similar to those reported as normal for both humans and other Callitrichidae species. The study of serum biochemical parameters in tamarins with experimental hepatitis A indicated that serum enzyme activities alone reflected the hepatic damage, while other biochemical parameters were of no real clinical importance. The experimental results showed the levels of serum urea to be indicative of the pathological involvement of the kidneys in experimental hepatitis A in some cases.
Subject(s)
Disease Models, Animal , Hepatitis A/blood , Monkey Diseases/blood , Saguinus/blood , Animals , Antigens, Viral/analysis , Feces/chemistry , Female , Hepatitis A/immunology , Hepatovirus/immunology , Male , Monkey Diseases/immunology , Reference Values , Sex CharacteristicsABSTRACT
A comparative study of hepatitis infection caused by human hepatitis A virus (MS-1 strain), simian hepatitis A virus (AGM-27 strain), and enterically transmitted non-A, non-B hepatitis virus (Tashkent-1435 strain) was carried out. Susceptibility of tamarins to the AGM-27 and Tashkent-1435 as well as to MS-1 strain was demonstrated. All the strains induced an acute infection characterized by serum alanine aminotransferase (ALT) elevation, virus excretion and antibody response. Certain differences in the course of infection caused by these strains were observed in the duration of the incubation period and ALT profiles.
Subject(s)
Disease Models, Animal , Hepatitis A/etiology , Hepatitis E/etiology , Saguinus , Alanine Transaminase/blood , Animals , Antibodies, Viral/blood , Antigens, Viral/analysis , Chlorocebus aethiops/microbiology , Feces/microbiology , Female , Hepatitis A/enzymology , Hepatitis A/microbiology , Hepatitis E/microbiology , Hepatovirus/immunology , Hepatovirus/isolation & purification , Humans , Immunoglobulin M/analysis , Male , Time FactorsABSTRACT
An experimental batch of inactivated hepatitis A vaccine was prepared using hepatitis A virus (HAV), HAS-15 strain, adapted to cell culture and purified by ultracentrifugation. The vaccine was tested in tamarins immunized intramuscularly three times one month apart. Three tamarins received a vaccine preparation containing 10 ng of immunogen each, three--100 ng each, and three animals were used as controls. The efficacy was judged by the anti-HAV antibody response in the vaccinated animals and development of immunity to subsequent virus challenge two months after the last immunization. The criteria of infection were: elevation of serum alanine aminotransferase, fecal excretion of HAV and production of specific antibody of IgM class. The immune response to 10 ng of the immunogen was lower than to 100 ng, however, both doses produced complete resistance to infection. The booster effect was observed in animals receiving 10 ng of the immunogen. The vaccine batch under study in the indicated doses was shown to have a good immunogenic potency and protective activity for tamarins.
