ABSTRACT
BACKGROUND: The purpose of the present study was to determine the effects of polyglycolic acid bioabsorbable membrane and oxidised cellulose on the osteogenesis in bone defects. MATERIALS AND METHODS: Fifty-four male adult New Zealand White rabbits weighing 3.0 to 3.5 kg were used. The rabbits were randomly divided into three groups of eighteen rabbits: the control group (n = 18), the polyglycolic acid bioabsorbable membrane group (biofix group, n = 18), and the oxidised cellulose group (Surgicel group, n = 18). Each experimental group was also randomly subdivided into two subgroups of nine rabbits, one with periosteum and the other without periosteum. A rectangular transosseous calvarial bone defect, 16 x 20 mm in size, was created. In the control group, the calvarial bone defect was not filled. Polyglycolic acid bioabsorbable membrane (Biofix, Bioscience, Tampere Finland) was implanted over the bone defect area in the biofix group, and oxidised cellulose (Surgicel, Ethicone, London, United Kingdom) in the Surgicel group. The calvarial bone defects were evaluated-histopathologically in the second, sixth, and tenth weeks. RESULTS: As for the results of sequential bone regeneration, macroscopical and histological aspects were observed. Bone formation was significantly enhanced by oxidised cellulose in the calvarial bone defects covered with periosteum, whereas cartilage formation was observed in bare calvarial bone defects. CONCLUSION: We believe that oxidised cellulose has osteogenic potential, and that polyglycolic acid bioabsorbable membrane inhibits osteoinductive effect and retards osteogenesis.
Subject(s)
Biocompatible Materials/pharmacology , Bone Regeneration/drug effects , Cellulose, Oxidized/pharmacology , Hemostatics/pharmacology , Osteogenesis/drug effects , Polyglycolic Acid/pharmacology , Animals , Male , Rabbits , SkullABSTRACT
The effects of an intravenous infusion of L-arginine as a physiological precursor of endothelium-derived relaxing factor/nitric oxide (EDRF/NO), iloprost (a stable prostacyclin (PGI2) analogue), and L-arginine combined with iloprost on skin viability were studied in 9 x 3 cm random pattern skin flaps in rats. Intravenous infusion of all drugs was started at the beginning of the operation and continued for 60 minutes. At the end of infusion period the depth of fluorescein dye penetration in the skin flap was assessed visually from photographic records, and the flap survival area was measured by the grid method at the seventh postoperative day. There was a significant reduction in distal necrosis of random skin flaps after intravenous infusion of L-arginine, iloprost, and L-arginine combined with iloprost (p < 0.01). Possible mechanisms that may be responsible for impairment of endothelium-dependent vasodilation and vasospasm in the microvasculature of random skin flap and their prevention with L-arginine and iloprost include restoration of the depleted stores of NO which in turn causes vasodilatation and has an antithrombotic effect.
Subject(s)
Arginine/pharmacology , Graft Survival/drug effects , Iloprost/pharmacology , Nitric Oxide/physiology , Surgical Flaps/blood supply , Vasodilator Agents/pharmacology , Animals , Drug Therapy, Combination , Male , Microcirculation/drug effects , Necrosis , Rats , Rats, WistarABSTRACT
Congenital absence of a vagina is a rare but outstanding anomaly of plastic surgery. Although many methods are described for reconstruction of vaginal agenesis, there is not a method yet to be approved as a perfect solution to this problem. With the aim of solving the problems faced with conventional methods, free jejunal transplantation was planned to construct a neovagina. For this method, we isolated a 15-cm jejunal segment, with its pedicle, transferred it to the preprepared vaginal pouch, and anastomosed the donor vessels of the mesentery to the inferior epigastric vessels. In the postoperative sixth month, problems of intercourse or the need to use any lubricator or stent were still not experienced and reported. In this paper, we judge and compare our approach by reviewing others.
Subject(s)
Jejunum/transplantation , Surgical Flaps/methods , Vagina/abnormalities , Adult , Anastomosis, Surgical/methods , Arteries/surgery , Female , Follow-Up Studies , Humans , Microsurgery/methods , Postoperative Care , Treatment Outcome , Vagina/blood supply , Vagina/surgeryABSTRACT
In a case with a large forehead defect following tight scalp bandaging in childhood, a prefabricated microvascular shoulder free flap was used for reconstruction. A free forearm fascial flap, with the radial artery and the concomitant vein as pedicle, was harvested and inserted under a subcutaneous pocket opened in the left shoulder region which served as the future "prefabricated free flap". In addition, a tissue expander was placed in this pocket to provide the necessary tissue expansion to enable primary donor site closure.
Subject(s)
Cerebral Hemorrhage/surgery , Craniotomy , Postoperative Complications/surgery , Scalp/surgery , Skin Transplantation , Surgical Flaps/methods , Adolescent , Female , Humans , Microsurgery/methods , Necrosis , Scalp/pathology , Suture Techniques , Tissue Expansion DevicesABSTRACT
An experimental study in 24 lambs was carried out to find an alternative tissue for the distal epiphyseal cartilage of the femur. The animals were divided into three groups. In the first group (n = 12) the left femoral distal epiphyseal cartilage was completely removed and replaced by a free perichondrial graft taken from the distal half of the scapula. In the second group (n = 6) the epiphyseal cartilage was removed and was not replaced by any tissue. The third group (n = 6) was used as control. After six months the legs in the first and third groups were of the same normal length while the legs in the second group were significantly shorter. We conclude that free perichondrial graft seems to be able to replace the epiphyseal cartilage and prevent retardation of growth.
