ABSTRACT
Giardiasis was detected in 53.5% of dogs examined by FASTest Giardia Strip for use in dogs. Using the ProSpecT Giardia EZ Microplate Assay 52.2% of these results was confirmed. Cysts of Giardia spp. were found only in 6.5% of samples of feces examined by flotation or decantation techniques. The examinations confirmed problems with coproscopic diagnosis of giardiasis in dogs. They confirmed the greater usefulness of FASTest Giardia Strip for immunodiagnostic of giardiasis in carnivores.
Subject(s)
Dog Diseases/diagnosis , Enzyme-Linked Immunosorbent Assay/veterinary , Giardia/isolation & purification , Giardiasis/veterinary , Animals , Dog Diseases/blood , Dog Diseases/parasitology , Dogs , Feces/parasitology , Female , Giardia/immunology , Giardiasis/diagnosis , Male , Predictive Value of Tests , Reagent Kits, Diagnostic/veterinaryABSTRACT
The purpose of this paper was to study the antigenic structure of Dicrocoelium dendriticum with a particular consideration of its antigenic relationship between F. hepatica and Paramphistomum sp. Studies of the antigenic composition of somatic extract of D. dendriticum and its four fractions obtained by gel separation chromatography on Sephadex G 75 were carried out by using double diffusion reaction with homologous (SDd) and heterogeneous (SFh, SPs) rabbit sera as well as sera of sheep naturally infected with liver fluke. The highest seroantigenic activity was observed for the somatic extract and its fraction I. A distinct antigenic relationship was found between the fluke species studied, a closer relationship between D. dendriticum and F. hepatica than between D. dendriticum and Paramphistomum sp. The studied sera of 7 sheep naturally infected with liver fluke were inactive in double diffusion reaction with D. dendriticum extract and its fractions.
