ABSTRACT
OBJECTIVE: Gastric cancer is a common kind of gastrointestinal malignancies. Increasing evidence indicates dysregulation of microRNA-99a (miR-99a) in gastric cancer, and has been extensively investigated in terms of cancer formation, progression, diagnosis, therapy, and prognosis. The purpose of this study is to explore how miR-99a worked in gastric cancer on migration and invasion. PATIENTS AND METHODS: The mRNA and protein levels of miR-99a and insulin-like growth factor 1 receptor (IGF1R) in gastric cancer were measured by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot. Transwell assay was employed to analyze the migratory and invasive capacities. The Dual-Luciferase reporter assay was performed to confirm miR-99a mediated the expression of IGF1R by directly targeting its mRNA 3'-untranslated regions (3'-UTR) in gastric cancer cells. RESULTS: MiR-99a was discovered to be significantly downregulated while IGF1R was upregulated in gastric cancer tissues and cell lines. The expression of miR-99a had a negative correlation with the IGF1R expression in gastric cancer tissues. Moreover, miR-99a was low expressed in gastric cancer cells HGC-27 and MGC-803 compared to the normal cell line. MiR-99a suppressed the migration and invasion through directly binding to the 3'-UTR of IGF1R mRNA in HGC-27 cells. In addition, IGF1R could reverse partial roles of miR-99a on migration and invasion in gastric cancer. CONCLUSIONS: MiR-99a inhibited the migratory and invasive abilities by regulating the expression of IGF1R. MiR-99a was downregulated while IGF1R was upregulated in gastric cancer cell lines. The newly identified miR-99a/IGF1R axis provides novel insight into the pathogenesis of gastric cancer.
Subject(s)
MicroRNAs/genetics , Receptor, IGF Type 1/genetics , Receptor, IGF Type 1/metabolism , Stomach Neoplasms/genetics , 3' Untranslated Regions , Cell Line, Tumor , Cell Movement , Cell Proliferation , Down-Regulation , Gene Expression Regulation, Neoplastic , Humans , Neoplasm Invasiveness , Stomach Neoplasms/metabolism , Up-RegulationABSTRACT
18 patients with acute promyelocytic leukemia (APL) were treated with HATP (Harringtonine, Adriamycin, Thioguanine, Prednisone) chemotherapy combined with chinese traditional medications. These medications are known to strengthen vital energy, promote blood circulation, remove stasis and clear toxic materials. 16 patients had complete remission (88.8%) and one partial remission with a total effective rate of 94.4%. Complete remission (CR) was achieved after 3 to 4 courses of treatment in most of the cases. 14 patients were still in CR at the completion of this study and the average duration of survival was 40.5 months. With the various therapeutic actions mentioned above, the traditional medications might decrease the toxicity of chemotherapy, reduce its side effects and prevent the occurrence of DIC. The combined use of traditional medications with chemotherapy may increase the rate and duration of CR as well as prolong the survival.