ABSTRACT
In order to meet the ground calibration requirements of optical detection equipment to identify optical characteristics of dim targets, an optical simulation method of dim targets based on passive detection link analysis and bidirectional scattering distribution function model is proposed. The off-axis collimation system for long focal length, the simulated energy transmission model of dim targets and the simplified model of bidirectional scattering distribution function are established. An internal stray light suppression baffle was designed to effectively suppress secondary scattering, and an optical simulation system for dim targets was built. The experimental results show that the system can simulate +7 Mvâ¼+20 Mv, and the simulation accuracy is better than 0.07 Mv. At the same time, the detection ability of the camera is tested by using the +15 Mv point simulated by the system. The signal-to-noise of the star point target reaches 6.7, which meets the requirements of detection rate and false alarm rate, and realizes the ground test of the camera's detection ability of the dim target.
ABSTRACT
The phosphatidylinositol 3-kinase (PI3K) signaling pathway plays important roles in cell proliferation, growth, and survival. Hyperactivated PI3K is frequently found in a wide variety of human cancers, validating it as a promising target for cancer therapy. We determined the crystal structure of the human PI3Kα-PI103 complex to unravel molecular interactions. Based on the structure, substitution at the R1 position of the phenol portion of PI103 was demonstrated to improve binding affinity via forming a new H-bond with Lys802 at the bottom of the ATP catalytic site. Interestingly, the crystal structure of the PI3Kα-9d complex revealed that the flexibility of Lys802 can also induce additional space at the catalytic site for further modification. Thus, these crystal structures provide a molecular basis for the strong and specific interactions and demonstrate the important role of Lys802 in the design of novel PI3Kα inhibitors.
ABSTRACT
OBJECTIVE: The purpose of this research is trying to uncover the biosynthetic or metabolic relative protein of thuringiensis by proteomics. METHODS: We conducted differential expression analysis between the high thuringiensin-yield Bacillus thuringiensis strain CT-43 and its mutants, high production strain CT-43-1C and non-production strain BMB0806, based on the 2-D gel. Then through mass spectrum (MS) identification and bio-informatics we analyzed the detected proteins. RESULTS: Thirteen spots were selected to be detected by the MS, and nine of them were identified. Among them, six proteins including in the basal metabolism pathway were possibly deduced that relative with the synthesis or metabolism of thuringiensin production. CONCLUSION: There were six proteins found to be connected with the influential role protein of biosynthesis and metabolic of thuringiensin production by comparative proteomics. This research provides the evidence of thuringiensis gene cluster cloning and biosynthetic analysis.
