The association between the triglyceride-glucose index and in-stent restenosis in patients undergoing percutaneous coronary intervention: a systematic review and meta-analysis.

BACKGROUND: Insulin resistance (IR) can lead to cellular metabolic disorders, activation of oxidative stress, and endothelial dysfunction, contributing to in-stent restenosis (ISR). The triglyceride-glucose index (TyG index), a new indicator reflecting IR, is extensively researched in the cardiovascular field. This study, through a meta-analysis, aimed to utilize a larger combined sample size and thereby enhance the overall test efficacy to explore the TyG index-ISR relationship. METHODS: A thorough search was conducted in the PubMed, EMBASE, Web of Science, and Cochrane Library databases to find original papers and their references published between 1990 and January 2024. This search included both prospective and retrospective studies detailing the correlation between the TyG index and ISR in individuals with coronary heart disease (CHD). OUTCOMES: The five included articles comprised 3,912 participants, and the odds ratio (OR) extracted from each study was combined using the Inverse Variance method. Results showed that, in the context of CHD patients, each incremental unit in the TyG index, when treated as a continuous variable, corresponded to a 42% elevation in ISR risk (95% CI 1.26-1.59, I²=13%, p < 0.005). When analyzing the TyG index categorically, the results revealed a higher ISR risk in the highest TyG index group compared to the lowest group (OR: 1.69, 95% CI 1.32-2.17, I²=0). Additionally, in patients with chronic coronary syndrome (CCS), each unit increase in the TyG index, the risk of ISR in patients increased by 37% (95% CI 1.19-1.57, I²=0%, p < 0.005). This correlation was also observable in acute coronary syndrome (ACS) patients (OR:1.48, 95% CI 1.19-1.85, I²=0, p < 0.005). CONCLUSIONS: The TyG index, an economical and precise surrogate for IR, is significantly linked with ISR. Furthermore, this correlation is unaffected by the type of coronary heart disease.

Facilitating stable gene integration expression and copy number amplification in Bacillus subtilis through a reversible homologous recombination switch.

Strengthening the expression level of integrated genes on the genome is crucial for consistently expressing key enzymes in microbial cell factories for efficient bioproduction in synthetic biology. In comparison to plasmid-based multi-copy expression, the utilization of chromosomal multi-copy genes offers increased stability of expression level, diminishes the metabolic burden on host cells, and enhances overall genetic stability. In this study, we developed the "BacAmp", a stabilized gene integration expression and copy number amplification system for high-level expression in Bacillus subtilis, which was achieved by employing a combination of repressor and non-natural amino acids (ncAA)-dependent expression system to create a reversible switch to control the key gene recA for homologous recombination. When the reversible switch is turned on, genome editing and gene amplification can be achieved. Subsequently, the reversible switch was turned off therefore stabilizing the gene copy number. The stabilized gene amplification system marked by green fluorescent protein, achieved a 3-fold increase in gene expression by gene amplification and maintained the average gene copy number at 10 after 110 generations. When we implemented the gene amplification system for the regulation of N-acetylneuraminic acid (NeuAc) synthesis, the copy number of the critical gene increased to an average of 7.7, which yielded a 1.3-fold NeuAc titer. Our research provides a new avenue for gene expression in synthetic biology and can be applied in metabolic engineering in B. subtilis.

Functional alterations in overweight/obesity: focusing on the reward and executive control network.

Overweight (OW) and obesity (OB) have become prevalent issues in the global public health arena. Serving as a prominent risk factor for various chronic diseases, overweight/obesity not only poses serious threats to people's physical and mental health but also imposes significant medical and economic burdens on society as a whole. In recent years, there has been a growing focus on basic scientific research dedicated to seeking the neural evidence underlying overweight/obesity, aiming to elucidate its causes and effects by revealing functional alterations in brain networks. Among them, dysfunction in the reward network (RN) and executive control network (ECN) during both resting state and task conditions is considered pivotal in neuroscience research on overweight/obesity. Their aberrations contribute to explaining why persons with overweight/obesity exhibit heightened sensitivity to food rewards and eating disinhibition. This review centers on the reward and executive control network by analyzing and organizing the resting-state and task-based fMRI studies of functional brain network alterations in overweight/obesity. Building upon this foundation, the authors further summarize a reward-inhibition dual-system model, with a view to establishing a theoretical framework for future exploration in this field.

Hairless (Hr) Deficiency Mitigates High-Fat Diet-Induced Obesity and Insulin Resistance in Mice.

Obesity is a significant global health concern linked to excessive dietary energy intake. This research focuses on the mammalian hairless protein (HR), known for its role in skin and hair function, and its impact on metabolism. Examining male wild-type (Hr+/+) and Hr null (Hr-/-) mice over a 14-week normal chow diet (NCD) or high-fat diet (HFD) intervention. This study reveals that HR deficiency exhibited a protective effect against HFD-induced obesity and insulin resistance. This protective effect is attributed to increased energy expenditure in Hr-/- mice. Moreover, the brown adipose tissue (BAT) of Hr-/- mice displays elevated levels of the thermogenic protein, uncoupling protein 1 (Ucp1), and its key transcriptional regulators (PPARγ and PGC1α), compared to Hr+/+ mice. In summary, the findings underscore the protective role of HR deficiency in countering HFD-induced adiposity by enhancing insulin sensitivity, raising energy expenditure, and augmenting thermogenic factors in BAT. Further exploration of HR metabolic regulation holds promise for potential therapeutic targets in addressing obesity-related metabolic disorders.

Neural 3D Scene Reconstruction with Indoor Planar Priors.

This paper addresses the challenge of reconstructing 3D indoor scenes from multi-view images. Many previous works have shown impressive reconstruction results on textured objects, but they still have difficulty in handling low-textured planar regions, which are common in indoor scenes. An approach to solving this issue is to incorporate planar constraints into the depth map estimation in multi-view stereo-based methods, but the per-view plane estimation and depth optimization lack both efficiency and multi-view consistency. In this work, we show that the planar constraints can be conveniently integrated into the recent implicit neural representation-based reconstruction methods. Specifically, we use an MLP network to represent the signed distance function as the scene geometry. Based on the Manhattan-world assumption and the Atlanta-world assumption, planar constraints are employed to regularize the geometry in floor and wall regions predicted by a 2D semantic segmentation network. To resolve the inaccurate segmentation, we encode the semantics of 3D points with another MLP and design a novel loss that jointly optimizes the scene geometry and semantics in 3D space. Experiments on ScanNet and 7-Scenes datasets show that the proposed method outperforms previous methods by a large margin on 3D reconstruction quality. The code and supplementary materials are available at https://zju3dv.github.io/ manhattan sdf.

Iontronic Dynamic Sensor with Broad Bandwidth and Flat Frequency Response Using Controlled Preloading Strategy.

Rapid advancements in human-machine interaction and voice biometrics impose desirability on soft mechanical sensors for sensing complex dynamic signals. However, existing soft mechanical sensors mainly concern quasi-static signals such as pressure and pulsation for health monitoring, limiting their applications in emerging wearable electronics. Here, we propose a hydrogel-based soft mechanical sensor that enables recording a wide range of dynamic signals relevant to humans by combining a preloading design strategy and iontronic sensing mechanism. The proposed sensor offers a two-orders-of-magnitude larger working bandwidth (up to 1000 Hz) than most of the reported soft mechanical sensors and meanwhile provides a high sensitivity (-23 dB) that surpasses the common commercial microphone. The amplitude-frequency characteristic of the proposed sensor can be precisely tuned to meet the desired requirement by adjusting the preloads and the parameters of the microstructured hydrogel. The sensor is capable of recording instrumental sounds with high fidelity from simple pure tones to melodic songs. Demonstration of a skin-mountable sensor used for human-voice-based remote control of a toy car shows great potential for applications in the voice user interface of human-machine interactions.

Rbfox1 regulates alternative splicing of Nrcam in primary sensory neurons to mediate peripheral nerve injury-induced neuropathic pain.

The primary sensory neurons of the dorsal root ganglia (DRG) are subject to transcriptional alterations following peripheral nerve injury. These alterations are believed to play a pivotal role in the genesis of neuropathic pain. Alternative RNA splicing is a process that generates multiple transcript variants from a single gene, significantly contributing to the complexity of the transcriptome. However, little is known about the functional significance and control of alternative RNA splicing in injured DRG after spinal nerve ligation (SNL). In our study, we conducted a comprehensive transcriptome profiling and bioinformatic analysis to approach and identified a neuron-specific isoform of an RNA splicing regulator, RNA-binding Fox1 (Rbfox1, also known as A2BP1), as a crucial regulator of alternative RNA splicing in injured DRG after SNL. Notably, Rbfox1 expression is markedly reduced in injured DRG following peripheral nerve injury. Restoring this reduction effectively mitigates nociceptive hypersensitivity. Conversely, mimicking the downregulation of Rbfox1 expression generates neuropathic pain symptoms. Mechanistically, we uncovered that Rbfox1 may be a key factor influencing alternative RNA splicing of neuron-glial related cell adhesion molecule (NrCAM), a key neuronal cell adhesion molecule. In injured DRG after SNL, the downregulation of Rbfox1amplifies the insertion of exon 10 in Nrcam transcripts, leading to an increase in long Nrcam variants (L-Nrcam) and a corresponding decrease in short Nrcam variants (S-Nrcam) within injured DRG. In summary, our study supports the essential role of Rbfox1 in neuropathic pain within DRG, probably via the regulation of Nrcam splicing. These findings suggest that Rbfox1 could be a potential target for neuropathic pain therapy.

Practical organic batteries: Concepts to realize high mass loading with high performance.

Organic electrode materials (OEMs) have been well developed in recent years. However, the practical applications of OEMs have not been paid sufficient attention. The concept here focused on one of the essential aspects for practical applications, i. e., high mass loading of active materials. This paper summarizes the challenges posed by high-mass loading of active materials in organic batteries and discusses the possible solutions in terms of organic electrode materials, conductive additives, electrode structures, and electrolytes or battery systems. We hope this concept can stimulate more attention to practical applications of organic batteries towards industry from lab.

High Q-factor Fano resonances based on a permittivity-asymmetric dielectric pea-shaped cylinder.

We numerically investigate two Fano resonances with high Q-factors based on a permittivity-asymmetric metastructure composed of two pea-shaped cylinders. By employing different materials to break the permittivity-asymmetry, the quasi-bound state of the continuum spectrum (BIC) resonance at 982.87 nm is excited, showing the Q-factor as high as 8183.7. The electromagnetic fields and vectors are analyzed by using the finite-difference time-domain (FDTD) method, and the resonance modes are identified as magnetic dipole (MD) responses and MDs by multipole decomposition in Cartesian coordinates, displaying that the light is confined within a pea-shaped cylinder to achieve localized field enhancement. In addition, the sensing performances of the metastructure are evaluated, and an optical refractive index sensor can be obtained with the sensitivity of 152 nm/RIU and maximum figure of merit (FOM) of 832.6. This proposed structure offers a new, to the best of our knowledge, way to achieve Fano resonant excitation on all-dielectric metastructures and can be used in nonlinear optics, biosensing, optical switches, and lasers.

Evaluation of starvation status in the early developmental stages of black rockfish (Sebastes schlegelii) based on morphological and histological characteristics.

Assessing the nutritional status and identifying major causes of mortality in larvae experiencing varying degrees of starvation are crucial for establishing appropriate feeding protocols and enhancing the welfare of hatchery-reared fish. The black rockfish Sebastes schlegelii is an important species in aquaculture and stock enhancement efforts in China, Japan, and Korea. This study aimed to identify optimal diagnostic morphometric indicators of starvation in newly hatched (0-6 days post-hatch, DPH) and postlarval stages (27-37 DPH) of this valuable fish species through histological analyses. Our findings revealed that certain morphometric parameters, including body length, the ratios of eye diameter to head height, body height to body length, and abdomen height to body height, exhibit sensitivity to starvation during both larval and postlarval stages. Particularly, the ratios of body height to body length and abdomen height to body height emerged as the most sensitive morphometric indicators of starvation. Histological examinations of the digestive system revealed rapid alterations in the morphology of hepatic parenchymal cells, accompanied by a significant decrease in the number of lipid cells in the liver during episodes of food deprivation. Starvation induced cellular degeneration in the digestive organs, manifested by reduced heights of epithelial cells and mucosal layers in the intestine, oesophagus, and stomach, along with degeneration and separation of muscle fibers. Among these variables, the height of the intestinal submucosa and muscle layer emerged as the most sensitive indicators reflecting nutritional conditions in newly hatched larvae. In contrast, the height of intestinal striated borders and mucosal folds proved to be the most sensitive indicators in the postlarval stage. Furthermore, the height of intestinal epithelial cells and the number of lipid vacuoles in enterocytes exhibited high sensitivity to food deprivation in both newly hatched larvae and postlarvae. These findings underscore the varying resilience of fish to starvation during different developmental phases and highlight the utility of morphological sensitivity characteristics as reliable diagnostic indices for assessing nutritional status in relation to starvation or suboptimal feeding during the early developmental stages of black rockfish in hatchery-reared processes.

Carbon Nanotube Network Topology-Enhanced Iontronic Capacitive Pressure Sensor with High Linearity and Ultrahigh Sensitivity.

Flexible capacitive pressure sensors with high sensitivity over a wide pressure range are highly anticipated in the fields of tactile perception and physiological signal monitoring. However, despite the introduction of microstructures on the electrolyte layer, the deformability is still limited due to the size limitation of the microstructures, making it difficult to significantly improve the sensitivity of iontronic capacitive pressure sensors (ICPSs). Here, we propose an innovative strategy of combining carbon nanotubes (CNTs) topological networks and ionic hydrogel micropyramid array microstructures that can significantly enhance the sensitivity of flexible ICPSs for ultrasensitive pressure detection. Compared with other previously reported ICPSs, the sensor developed in this work exhibits an unprecedented sensitivity (Smin > 1050 kPa-1) and a high linear response (R2 > 0.99) in a wide pressure range (0.03-28 kPa) enabled by CNT percolation networks inside the microstructred electrolyte layer. This ultrasensitive and flexible ICPS also can effectively detect pressure from a variety of sources, including sound waves, lightweight objects, and tiny physiological signals, such as pulse rate and heartbeat. This work provides a general strategy to achieve an ICPS with both broader pressure-response range and higher sensitivity, which provides a stable and efficient way for a low-cost, scalable sensor for sensitive tactile sensing in human-computer interaction applications.

Universal theory of strange metals from spatially random interactions.

Strange metals-ubiquitous in correlated quantum materials-transport electrical charge at low temperatures but not by the individual electronic quasiparticle excitations, which carry charge in ordinary metals. In this work, we consider two-dimensional metals of fermions coupled to quantum critical scalars, the latter representing order parameters or fractionalized particles. We show that at low temperatures (T), such metals generically exhibit strange metal behavior with a T-linear resistivity arising from spatially random fluctuations in the fermion-scalar Yukawa couplings about a nonzero spatial average. We also find a T ln(1/T) specific heat and a rationale for the Planckian bound on the transport scattering time. These results are in agreement with observations and are obtained in the large N expansion of an ensemble of critical metals with N fermion flavors.

Engineered bacterial orthogonal DNA replication system for continuous evolution.

Continuous evolution can generate biomolecules for synthetic biology and enable evolutionary investigation. The orthogonal DNA replication system (OrthoRep) in yeast can efficiently mutate long DNA fragments in an easy-to-operate manner. However, such a system is lacking in bacteria. Therefore, we developed a bacterial orthogonal DNA replication system (BacORep) for continuous evolution. We achieved this by harnessing the temperate phage GIL16 DNA replication machinery in Bacillus thuringiensis with an engineered error-prone orthogonal DNA polymerase. BacORep introduces all 12 types of nucleotide substitution in 15-kilobase genes on orthogonally replicating linear plasmids with a 6,700-fold higher mutation rate than that of the host genome, the mutation rate of which is unchanged. Here we demonstrate the utility of BacORep-based continuous evolution by generating strong promoters applicable to model bacteria, Bacillus subtilis and Escherichia coli, and achieving a 7.4-fold methanol assimilation increase in B. thuringiensis. BacORep is a powerful tool for continuous evolution in prokaryotic cells.

Effects of Two Environmental Enrichment Methods on Cognitive Ability and Growth Performance of Juvenile Black Rockfish Sebastes schlegelii.

A widely used approach to restoring marine fishery resources is stock enhancement using hatchery-reared fish. However, artificial rearing environments, which are often lacking in enrichment, may negatively affect the cognition, welfare, and adaptive capacity to new environments of juvenile fish, thereby leading to low post-release survival rates. This study examined the effects of habitat and social enrichment on the growth performance and cognitive ability of Sebastes schlegelii. Following seven weeks of environmental enrichment, a T-maze experiment was conducted, and the telencephalon and visceral mass of the fish were sampled to measure the growth (growth hormone: GH; insulin-like growth factor-1: IGF-1; and somatostatin: SS) and cognitive abilities (brain-derived neurotrophic factor: BDNF; and nerve growth factor: NGF)-related indicator levels. The results indicated that, although the final body length, final body weight, and specific growth rate of both enrichment groups were lower than those of the control group, both methods of enrichment had a positive impact on growth-related factors (increased GH, increased IGF-1, and decreased SS). The enrichment groups demonstrated a stronger learning ability in the T-maze test, and the levels of BDNF and NGF in the telencephalon were significantly higher in the enrichment groups than those in the control group. Additionally, there was a significant interaction between the two enrichment methods on the NGF level. This study confirms that a more complex and enriching environment is beneficial for cultivating the cognitive abilities of cultured juvenile S. schlegelii, and the result can provide a reference for the improvement of the stock enhancement of this species.

A Leucine-Rich Repeat Receptor-like Kinase TaBIR1 Contributes to Wheat Resistance against Puccinia striiformis f. sp. tritici.

Plant cell surface-localized receptor-like kinases (RLKs) recognize invading pathogens and transduce the immune signals inside host cells, subsequently triggering immune responses to fight off pathogen invasion. Nonetheless, our understanding of the role of RLKs in wheat resistance to the biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) remains limited. During the differentially expressed genes in Pst infected wheat leaves, a Leucine-repeat receptor-like kinase (LRR-RLK) gene TaBIR1 was significantly upregulated in the incompatible wheat-Pst interaction. qRT-PCR verified that TaBIR1 is induced at the early infection stage of Pst. The transient expression of TaBIR1-GFP protein in N. bentamiana cells and wheat mesophyll protoplasts revealed its plasma membrane location. The knockdown of TaBIR1 expression by VIGS (virus induced gene silencing) declined wheat resistance to stripe rust, resulting in reduced reactive oxygen species (ROS) production, callose deposition, and transcripts of pathogenesis-related genes TaPR1 and TaPR2, along with increased Pst infection area. Ectopic overexpression of TaBIR1 in N. benthamiana triggered constitutive immune responses with significant cell death, callose accumulation, and ROS production. Moreover, TaBIR1 triggered immunity is dependent on NbBAK1, the silencing of which significantly attenuated the defense response triggered by TaBIR1. TaBIR1 interacted with the NbBAK1 homologues in wheat, co-receptor TaSERK2 and TaSERK5, the transient expression of which could restore the impaired defense due to NbBAK1 silencing. Taken together, TaBIR1 is a cell surface RLK that contributes to wheat stripe rust resistance, probably as a positive regulator of plant immunity in a BAK1-dependent manner.

Molecular Design for Symmetric All-Organic Batteries.

Symmetric batteries that use the same material as both cathodes and anodes have several advantages. However, traditional inorganic materials are facing challenges as electrode materials in symmetric batteries. The designable organic electrode materials (OEMs) make it possible to fabricate symmetric all-organic batteries (SAOBs), which are still in their infancy stage. Herein, we summarize the requirements of the OEMs for SAOBs and classify SAOBs based on the types of OEMs (n-type and bipolar, including carbonyl materials, materials with C=N groups, conducting polymers, free radical compounds, conjugated coordination polymers, arylamine derivatives etc.). We review the recent progress on SAOBs and analyze the advantages and shortcomings of different types of SAOBs. The strategies for designing OEMs in SAOBs with high performance are discussed. Thus, we hope this review will stimulate more interest in SAOBs and pave the potential application of SAOBs with high performance.

Biomimetic cell membrane-coated nanocarriers for targeted siRNA delivery in cancer therapy.

Small interfering RNA (siRNA) therapeutics for cancer are a focus of increasing research interest. However, the major obstacle to their clinical application is the targeted delivery of siRNA to cancer cells at desirable levels. Cell membrane-coated nanocarriers have the advantage of combining the properties of both cell membranes and nanoparticles (NPs). In this review, we highlight the most common RNAi therapeutics and the extracellular and intracellular barriers to siRNA delivery. Moreover, we discuss clinical applications of different cell membrane-coated nanocarriers for targeted siRNA delivery, including cancer cell membranes (CCMs), platelet membranes, erythrocyte membranes, stem cell membranes, exosome membranes, and hybrid membranes. Taken together, biomimetic cell membrane-coated nanotechnology is a promising strategy for targeted siRNA delivery for cancer treatment.

Sensitive Small Molecule Aptasensing based on Hybridization Chain Reaction and CRISPR/Cas12a Using a Portable 3D-Printed Visualizer.

Next-generation biosensing tools based on CRISPR/Cas have revolutionized the molecular detection. A number of CRISPR/Cas-based biosensors have been reported for the detection of nucleic acid targets. The establishment of efficient methods for non-nucleic acid target detection would further broaden the scope of this technique, but up to now, the concerning research is limited. In the current study, we reported a versatile biosensing platform for non-nucleic acid small-molecule detection called SMART-Cas12a (small-molecule aptamer regulated test using CRISPR/Cas12a). Simply, hybridization chain reaction cascade signal amplification was first trigged by functional nucleic acid (aptamer) through target binding. Then, the CRISPR/Cas system was integrated to recognize the amplified products followed by activation of the trans-cleavage. As such, the target can be ingeniously converted to nucleic acid signals and then fluorescent signals that can be readily visualized and analyzed by a customized 3D-printed visualizer with the help of a home-made App-enabled smartphone. Adenosine triphosphate was selected as a model target, and under the optimized conditions, we achieved fine analytical performance with a linear range from 0.1 to 750 µM and a detection limit of 1.0 nM. The satisfactory selectivity and recoveries that we have obtained further demonstrated this method to be suitable for a complex sample environment. The sample-to-answer time was less than 100 min. Our work not only expanded the reach of the CRISPR-Cas system in biosensing but also provided a prototype method that can be generalized for detecting a wider range of analytes with desirable adaptability, sensitivity, specificity, and on-site capability.

Association between total cholesterol and total bone mineral density in US adults: National Health and Nutrition Examination Survey (NHANES), 2011-2018.

BACKGROUND: Accumulated evidence indicates that cholesterol is offensive to bone metabolism. Therefore, we examined the real-world study among total cholesterol and total bone mineral density (BMD). We investigated the relationship between total cholesterol and total BMD among 10,039 US participants aged 20-59 years old over the period 2011-2018 from the NHANES. METHODS: To analyze the relationship among total cholesterol and total BMD, multivariate linear regression models were used. Fitted smoothing curves, generalized additive models, and threshold effect analysis were also conducted. RESULTS: After adjusting for additional covariates, weighted multivariable linear regression models indicated total cholesterol concentration levels exhibited a negative relationship with total BMD, particularly among participants aged 20-29 years. Concerning subgroup analysis, stratified by gender, race/ethnicity and age group, the negative correlation of total cholesterol with total BMD dwelled in both female and male as well as in whites and other races (including Hispanic and Multi-Racial), but not in non-Hispanic blacks and Mexican American. In other races, this relationship presented a nonlinear association (inflection point: 6.7 mmol/L) with a U-shaped curve. Among participants aged 40 to 49 years, this relationship also followed a nonlinear association (inflection point: 5.84 mmol/L), indicating a saturation effect. Moreover, the three types of diabetes status were found to have negative, U-shaped, and positive relationships. In participants with borderline diabetes status, the relationship of total cholesterol with total BMD was a U-shaped curve (inflection point: 4.65 mmol/L). CONCLUSIONS: For US young adults (20-29 years old), our study revealed a negative relationship between total cholesterol and total BMD. This association followed a U-shaped curve (inflection point: 4.65 mmol/L) in borderline diabetes status participants, a saturation curve (inflection point: 5.84 mmol/L) in participants aged 40-49 years and a nonlinear curve (inflection point: 6.7 mmol/L) in other races (including Hispanic and Multi-Racial). Therefore, keeping total cholesterol concentration at a reasonable level for young adults and diabetic population might be an approach to prevent osteoporosis or osteopenia.