ABSTRACT
We aimed to compare the performance of acellular nerves prepared by different decellularization methods, screening out the optimal decellularization protocol, repairing the sciatic nerve defects in rats by the allogeneic transplantation, and evaluating the effect of regenerative nerve on the function reconstruction. The Sondell, SB-SDS, TnBP, and the high/low permeation methods were used to decellularize donor nerves. Nerves without any treatment were as the control group. The histological results were evaluated by HE staining and toluidine blue (TB) staining. The proliferation activity of L929 cells was detected by CCK-8 assay. The adhesion of Schwann cells was observed and quantified by SEM. Balb/c mice were used to evaluate the cellular and humoral immunogenicity of the nerve scaffolds. The rat sciatic nerve defect model was applied to observe the repair effect of acellular nerve scaffold in vivo. To SB-SDS group, it remained the original state of the nerves, with no observed nucleus and axons, the neurotoxicity grade detected by CCK-8 being almost 0, and it kept the largest number of Schwann cells adhered to the acellular nerve and the better morphology. Further, it showed that the selected SB-SDS rats acellular nerve scaffold could promote the nerve repair of the rats by HE staining and TB staining. We could conclude that the acellular nerve matrix prepared by the SB-SDS method effectively removes the cellular components in the nerve tissue and retains the main components of the extracellular matrix of the nerve tissue, whose rats decellularized nerve scaffold could promote the sciatic nerve repair better.
Subject(s)
Sciatic Nerve/transplantation , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Tissue Transplantation/methods , Animals , Cell Adhesion , Cells, Cultured , Detergents/chemistry , Detergents/pharmacology , Mice , Mice, Inbred BALB C , Nerve Regeneration , Rats , Rats, Sprague-Dawley , Schwann Cells/physiology , Sciatic Nerve/drug effects , Sciatic Nerve/injuries , Tissue Scaffolds/adverse effects , Transplantation, HomologousABSTRACT
Eelgrass (Zostera marina), a seagrass species widely distributed in the coastal regions of northern hemisphere, has suffered with a great decline due to a variety of anthropogenic and environmental stresses. In order to examine the adaptability of eelgrass to different environmental stresses, studies on the morphology and reproductive capacity of eelgrass had been carried out monthly from November 2014 to October 2015 at four different habitats of the Swan Lake, including patch area inintertidal area and subtidal area, eelgrass meadow edge, and eelgrass meadow area. The results showed significant spatio-temporal variations in the morphological parameters and branch frequency of eelgrass shoots at different habitats of the Swan Lake. The highest values of leaf length, leaf width, aboveground/belowground biomass, and internode length/diameter were observed in the meadow area, i.e., 78.54 cm, 7.93 mm, 7.03 and 3.88, respectively, while the highest branch frequency was observed in the meadow edge (88.4%). The plasticity index for aboveground/belowground biomass was higher (ranging from 0.77 to 0.92) at the four habitats, but those for the leaf width was slightly lower (ranging from 0.41 to 0.64). The number of spathes in each shoot showed no significant difference at different habitats, whereas the number of spathes per unit area was significantly different. Clonal reproduction was more dominant in meadow area than in the patch area where human disturbance was high.