ABSTRACT
Invisible aligners have been widely used in orthodontic treatment but still present issues with plaque formation and oral mucosa abrasion, which can lead to complicated oral diseases. To address these issues, hydrophilic poly(sulfobetaine methacrylate) (polySBMA) coatings with lubricating, antifouling, and antiadhesive properties have been developed on the aligner materials (i.e., polyethylene terephthalate glycol, PETG) via a simple and feasible glycidyl methacrylate (GMA)-assisted coating strategy. Poly(GMA-co-SBMA) is grafted onto the aminated PETG surface via the ring-opening reaction of GMA (i.e., "grafting to" approach to obtain G-co-S coating), or a polySBMA layer is formed on the GMA-grafted PETG surface via free radical polymerization (i.e., "grafting from" approach to obtain G-g-S coating). The G-co-S and G-g-S coatings significantly reduce the friction coefficient of PETG surface. Protein adsorption, bacterial adhesion, and biofilm formation on the G-co-S- and G-g-S-coated surfaces are significantly inhibited. The performance of the coatings remains stable after storage in air or artificial saliva for 2 weeks. Both coatings demonstrate good biocompatibility in vitro and is not caused irritation to the oral mucosa of rats in vivo over 2 weeks. This study proposes a promising strategy for the development of invisible aligners with improved performance, which is beneficial for oral health treatment.
ABSTRACT
Anopheles sinensis adults were captured from Huaibin County and raised in laboratory. Larvae dipping method and the WHO tubes were used to determine the resistance of F1 IV-stage larvae and 3-5 day-old adult mosquitoes to dimethyl dichlorovinyl phosphate (DDVP), deltamethrin, and arprocarb. The activity of acetylcholine esterase (AChE) of larvae and adult mosquitoes was measured by colorimetric method before and after the respective addition of the 3 insecticides. The results showed that the mortality of adult mosquitoes in 0.05% deltamethrin testing tube (0.017 8 g/m2) was 51.2%, which was less than 80% and judged as resistant group. The LC50 of larvae to DDVP, deltamethrin, and arprocarb were 2.76, 320.85, 754.32 mg/L, respectively. The inhibition rates of 0.005 mmol/L DDVP, deltamethrin, and arprocarb to AChE activity were 77.8%, 62.8%, and 58.9% in larvae, while 76.1%, 62.2%, and 58.22% in adults, respectively, showing a significant inhibition on the AChE activity of An. sinensis (P < 0.01).
