ABSTRACT
OBJECTIVE: This study aimed to examine the role of long non-coding RNA PCED1B antisense RNA 1 (PCED1B-AS1) in the development of hepatocellular carcinoma (HCC). METHODS: A total of 62 pairs of HCC tissues and adjacent non-tumor tissues were obtained from 62 HCC patients. The interactions of PCED1B-AS1 and microRNA-34a (miR-34a) were detected by dual luciferase activity assay and RNA pull-down assay. The RNA expression levels of PCED1B-AS1, miR-34a and CD44 were detected by RT-qPCR, and the protein expression level of CD44 was determined by Western blotting. The cell proliferation was detected by cell proliferation assay, and the cell invasion and migration by transwell invasion assay. The HCC tumor growth after PCED1B-AS1 was downregulated was determined by in vivo animal study. RESULTS: PCED1B-AS1 was highly expressed in HCC tissues, which was associated with poor survival of HCC patients. Furthermore, PCED1B-AS1 interacted with miR-34a in HCC cells, but they did not regulate the expression of each other. Additionally, PCED1B-AS1 increased the expression level of CD44, which was targeted by miR-34a. The cell proliferation and invasion assay revealed that miR-34a inhibited the proliferation and invasion of HCC in vitro, while CD44 exhibited the opposite effects. Furthermore, PCED1B-AS1 suppressed the role of miR-34a. Moreover, the knockdown of PCED1B-AS1 repressed the HCC tumor growth in nude mice in vivo. CONCLUSION: PCED1B-AS1 may play an oncogenic role by regulating the miR-34a/CD44 axis in HCC.
Subject(s)
Carcinoma, Hepatocellular , Cell Proliferation , Gene Expression Regulation, Neoplastic , Hyaluronan Receptors , Liver Neoplasms , MicroRNAs , Neoplasm Invasiveness , RNA, Long Noncoding , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/pathology , Carcinoma, Hepatocellular/metabolism , MicroRNAs/genetics , MicroRNAs/metabolism , Humans , Hyaluronan Receptors/genetics , Hyaluronan Receptors/metabolism , Liver Neoplasms/genetics , Liver Neoplasms/pathology , Liver Neoplasms/metabolism , Cell Proliferation/genetics , RNA, Long Noncoding/genetics , RNA, Long Noncoding/metabolism , Animals , Mice , Neoplasm Invasiveness/genetics , Male , Cell Line, Tumor , Female , Cell Movement/genetics , Middle Aged , Mice, Nude , RNA, Antisense/geneticsABSTRACT
Well-performing organic-inorganic halide perovskites are susceptible to poor efficiency and instability due to their various defects at the interphases, grain boundaries (GBs), and surfaces. In this study, an in situ method is utilized for effectively passivating the under-coordinated Pb2+ defects of perovskite with new non-fullerene acceptors (NFAs) (INXBCDT; X = H, Cl, and Br) through their carbonyl and cyano functional groups during the antisolvent dripping process. It reveals that the bicyclopentadithiophene (BCDT) core with highly electron-withdrawing end-capping groups passivates GBs and boosts perovskite grain growth. This effective defect passivation decreases the trap density to increase the carrier recombination lifetime of the perovskite film. As a result, bromo-substituted dicyanomethylene indanone (INBr)-end-capped BCDT (INBrBCDT-b8; 3a)-passivated devices exhibit the highest power conversion efficiency (PCE) of 22.20% (vs those of 18.09% obtained for perovskite films without passivation) upon an optimized film preparation process. Note that devices treated with more soluble 2-ethylhexyl-substituted compounds (1a, 2a, and 3a) exhibit higher PCE than those treated with less soluble octyl-substituted compounds (1b, 2b, and 3b). It is also worth noting that BCDT is a cost-effective six-ring core that is easier to synthesize with a higher yield and therefore much cheaper than those with highly fused-ring cores. In addition, a long-term stability test in a glovebox for 1500 h reveals that the perovskite solar cells (PSCs) based on a perovskite absorber treated with compound 3a maintain â¼90% of their initial PCE. This is the first example of the simplest high-conjugation additive for perovskite film to achieve a PCE greater than 22% of the corresponding lead-based PSCs.
ABSTRACT
Honeysuckle is a lonicera plant, commonly used traditional precious raw material for medicine in China, and is a commonly used Chinese native medicine and an important material for cold drink. Honeysuckle has the effect of alleviating fever and disintoxicating, and is used mainly to treat the carbuncular swelling, and cure boils, throat rheumatism, erysipelas, painfully hot sun blood dysentery, cold, and various diseases accompanied by fever. The simultaneous determination of multi-metal elements in honeysuckle was studied by inductively coupled plasma atomic emission spectrum (ICP-AES) after carbon pick-up acid dissolvation digestion. The average recovery of the method for Cu, Mn, Ni, Pb, Fe, Cr, Ca and Mg in honeysuckle was found to be 83.8%-115.6%, while RSD (n = 10) is 1.1%-4.9% for the multi-elements in honeysuckle. The method was applied to the analysis of some practical samples and the results obtained were satisfactory. Fe and Mn are rather rich in honeysuckle. It is of important significance to assess the value of the food cooked with medicinal herbs by the determination of multi-trace elements.
Subject(s)
Lonicera/chemistry , Metals/analysis , Calcium/analysis , Carbon/chemistry , Chromium/analysis , Copper/analysis , Iron/analysis , Lead/analysis , Magnesium/analysis , Manganese/analysis , Nickel/analysis , Nitric Acid/chemistry , Perchlorates/chemistry , Reproducibility of Results , Spectrophotometry, Atomic/methodsABSTRACT
Determination of metal elements in buds of Syzygium aromaticum (L. )Merr. et Perry was studied by ICP-AES after HNO3-H2O2 digestion. The average recovery of the method for K, Mg, and trace elements such as Cu, Fe, Cr, Zn, Mn and Pb in the buds of Syzygium aromaticum (L.) Merr. et Perry was found to be 91.8% -112.2%, while RSD (n = 5) is 0.4% - 4.9% for the metal elements. The method was applied to the analysis of some practical samples, and the results obtained were satisfactory. There is rich Mn in the samples. It is of important significance to assess the value of the Chinese medicine by the determination of trace elements in Chinese medicine with ICP-AES.
Subject(s)
Metals/analysis , Spectrophotometry, Atomic/methods , Syzygium/chemistryABSTRACT
The determination of metal elements in Chinese medicine wild jujube seed decoction was studied by ICP-AES after HNO3-H2O2 digestion. The average recoveries of the method for Ca, Cr, Al, Fe, K, Mg, Mn and Zn in wild jujube seed decoction were found to be 95.5%-106.8%, while RSD (n=5) was found to be 1.7%-3.9% for these metal elements. The method was applied to the analysis of some practical samples and the results obtained were satisfactory.
Subject(s)
Drugs, Chinese Herbal/analysis , Metals/analysis , Spectrophotometry, Atomic/methods , Ziziphus/chemistry , Metals/isolation & purificationABSTRACT
Calcium is the necessity element in human body and its content has effect on exciting muscle or nerve. Calcium is important in the physiologic process of muscle or nervous reaction, nervous impulse transmission, heartbeat rhythm maintenance, blood solidification, cell coherence and so on. Determination of calcium element in Chinese medicine wild jujube seed decoction and radix puerariae was studied by ICP-AES after they were digested with HNO3-HClO4. The precision (RSD) is 1.7%. The average recovery of calcium in wild jujube seed decoction is 97.7% and in radix puerariae is 104.4%. Calcium was abundant in Chinese medicine and could play certain pharmacologic role in Chinese medicine. It is of important significance to assess the value of Chinese medicine by determination of calcium element in Chinese medicine with ICP-AES.
